scholarly journals Capped mRNAs with Reduced Secondary Structure Can Function in Extracts from Poliovirus-Infected Cells

1982 ◽  
Vol 2 (12) ◽  
pp. 1633-1638 ◽  
Author(s):  
Nahum Sonenberg ◽  
Denise Guertin ◽  
Kevin A. W. Lee
Keyword(s):  
Secondary Structure ◽  
Mosaic Virus ◽  
Hela Cells ◽  
Alfalfa Mosaic Virus ◽  
Stable Secondary Structure ◽  
Ribosome Binding ◽  
Infected Cells

Extracts from poliovirus-infected HeLa cells were used to study ribosome binding of native and denatured reovirus mRNAs and translation of capped mRNAs with different degrees of secondary structure. Here, we demonstrate that ribosomes in extracts from poliovirus-infected cells could form initiation complexes with denatured reovirus mRNA, in contrast to their inability to bind native reovirus mRNA. Furthermore, the capped alfalfa mosaic virus 4 RNA, which is most probably devoid of stable secondary structure at its 5′ end, could be translated at much higher efficiency than could other capped mRNAs in extracts from poliovirus-infected cells.

Capped mRNAs with Reduced Secondary Structure Can Function in Extracts from Poliovirus-Infected Cells

1982 ◽  
Vol 2 (12) ◽  
pp. 1633-1638
Author(s):  
Nahum Sonenberg ◽  
Denise Guertin ◽  
Kevin A. W. Lee
Keyword(s):  
Secondary Structure ◽  
Mosaic Virus ◽  
Hela Cells ◽  
Alfalfa Mosaic Virus ◽  
Stable Secondary Structure ◽  
Ribosome Binding ◽  
Infected Cells

Extracts from poliovirus-infected HeLa cells were used to study ribosome binding of native and denatured reovirus mRNAs and translation of capped mRNAs with different degrees of secondary structure. Here, we demonstrate that ribosomes in extracts from poliovirus-infected cells could form initiation complexes with denatured reovirus mRNA, in contrast to their inability to bind native reovirus mRNA. Furthermore, the capped alfalfa mosaic virus 4 RNA, which is most probably devoid of stable secondary structure at its 5′ end, could be translated at much higher efficiency than could other capped mRNAs in extracts from poliovirus-infected cells.

The appearance of eight strains of alfalfa mosaic virus in alfalfa leaves and their effect on the ultrastructure of infected cells

1974 ◽  
Vol 52 (5) ◽  
pp. 979-985 ◽  
Author(s):  
Roy D. Wilcoxson ◽  
F. I. Frosheiser ◽  
Lois B. Johnson
Keyword(s):  
Mosaic Virus ◽  
Alfalfa Mosaic Virus ◽  
Leaf Tissue ◽  
Cell Organelles ◽  
Virus Particles ◽  
Vascular Parenchyma ◽  
Infected Cells ◽  
Medicago Sativa L ◽  
Ultrastructural Damage ◽  
Apparently Healthy

Eight strains of alfalfa mosaic virus (AMV) were studied by electron microscopy in alfalfa (Medicago sativa L.) leaf tissue and after purification. The virus occurred in the cytoplasm and occasionally in the vacuoles of mesophyll and vascular parenchyma cells; it was not associated with cell organelles. One strain of AMV (U5) did not incite symptoms in the alfalfa leaves and caused no ultrastructural damage to the infected cells. Two strains (U10 and U21) caused no symptoms in alfalfa, but the tonoplast of infected cells was not closely attached to the cytoplasm and floated in the vacuole; cell organelles were not damaged. The other five strains of AMV (F1, NY1, R6, B1, and W1) regularly or occasionally produced symptoms in alfalfa leaves. In leaves that were symptomless, as well as in the apparently healthy parts of leaves with symptoms of AMV infection, there was no apparent ultrastructural damage to the infected cells. Within the part of a leaf where there were symptoms, the tonoplast was detached from the cytoplasma and was folded within the vacuole in various patterns, along with bits of cytoplasm and virus particles. Cell organelles were often found in various stages of disintegration. Three different aggregations of the virus were recognized. The eight AMV strains were grouped into three general classes on the basis of the range in virus particle sizes. Mycoplasm was not associated with any of the AMV strains.

THESIS SUMMARY STUDIES OF ALFALFA MOSAIC VIRUS AND SUBTERRANEAN CLOVER RED LEAF VIRUS

1979 ◽  
pp. 163-164
Author(s):  
P.B. Teh
Keyword(s):  
Mosaic Virus ◽  
Alfalfa Mosaic Virus ◽  
Subterranean Clover ◽  
Test Plant ◽  
Transmission Frequency ◽  
Virus Source ◽  
Leaf Virus

AMV was shown to be transmitted by sap, aphids and through lucerne seed, but not by Cuscuta. Virus source and test plant influenced transmission frequency. Sap-inoculation tests showed that 20 species of plants were susceptible to this virus. Thirteen species of plants from the fields where AMV had been detected were tested but only three were found to be infected with the virus.

scholarly journals Identification, Characterization, and Molecular Detection of Alfalfa mosaic virus in Potato

2006 ◽  
Vol 96 (11) ◽  
pp. 1237-1242 ◽  
Author(s):  
H. Xu ◽  
J. Nie
Keyword(s):  
Mosaic Virus ◽  
Gene Sequence ◽  
Alfalfa Mosaic Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Polymorphism Analysis ◽  
Rt Pcr ◽  
Potato Leaf ◽  
Simple Method ◽  
Cp Gene ◽  
Nucleotide Sequence Alignment

Alfalfa mosaic virus (AMV) was detected in potato fields in several provinces in Canada and characterized by bioassay, enzyme-linked immunosorbent assay, and reverse-transcription polymerase chain reaction (RT-PCR). The identity of eight Canadian potato AMV isolates was confirmed by sequence analysis of their coat protein (CP) gene. Sequence and phylogenetic analysis indicated that these eight AMV potato isolates fell into one strain group, whereas a slight difference between Ca175 and the other Canadian AMV isolates was revealed. The Canadian AMV isolates, except Ca175, clustered together among other strains based on alignment of the CP gene sequence. To detect the virus, a pair of primers, AMV-F and AMV-R, specific to the AMV CP gene, was designed based on the nucleotide sequence alignment of known AMV strains. Evaluations showed that RT-PCR using this primer set was specific and sensitive for detecting AMV in potato leaf and tuber samples. AMV RNAs were easily detected in composite samples of 400 to 800 potato leaves or 200 to 400 tubers. Restriction analysis of PCR amplicons with SacI was a simple method for the confirmation of PCR tests. Thus, RT-PCR followed by restriction fragment length polymorphism analysis may be a useful approach for screening potato samples on a large scale for the presence of AMV.

scholarly journals Sequence-specific polypeptoids: A diverse family of heteropolymers with stable secondary structure

1998 ◽  
Vol 95 (8) ◽  
pp. 4303-4308 ◽  
Author(s):  
K. Kirshenbaum ◽  
A. E. Barron ◽  
R. A. Goldsmith ◽  
P. Armand ◽  
E. K. Bradley ◽  
...  
Keyword(s):  
Secondary Structure ◽  
Stable Secondary Structure

scholarly journals First report of the infection of alfalfa mosaic virus in Salvia sclarea in Hungary

2018 ◽  
Vol 100 (3) ◽  
pp. 607-607 ◽  
Author(s):  
Pal Salamon ◽  
Anita Sos-Hegedus ◽  
Peter Gyula ◽  
Gyorgy Szittya
Keyword(s):  
Mosaic Virus ◽  
Alfalfa Mosaic Virus ◽  
First Report ◽  
Salvia Sclarea
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