scholarly journals MCM1 binds to a transcriptional control element in Ty1.

1993 ◽  
Vol 13 (1) ◽  
pp. 57-62 ◽  
Author(s):  
B Errede
Keyword(s):  
Gene Expression ◽  
Transcriptional Control ◽  
Control Element ◽  
Regulatory Sequence ◽  
Gene Promoters ◽  
Adjacent Gene ◽  
Transposable Element Insertion ◽  
Transcriptional Regulatory ◽  
Transcriptional Regulatory Sequence ◽  
Insertion Mutations

Some Ty1 transposable-element insertion mutations of Saccharomyces cerevisiae activate adjacent-gene expression. These Ty1-activated genes are regulated similarly to certain mating genes. This report shows that the MCM1 protein, which binds to several mating genes, also binds to a transcriptional regulatory sequence in Ty1. The binding of MCM1 to Ty1 correlates with the ability of its binding site to function as a component of the Ty1 transcriptional activator. This correlation supports the idea that MCM1 is important for Ty1-activated gene expression. At mating-gene promoters, MCM1 binds with coactivators or repressors such as STE12, alpha 1, or alpha 2. In contrast, MCM1 binds without these associated DNA-binding proteins at its site in Ty1. This finding suggests that its role in Ty1-mediated transcription is different from that at mating genes.

MCM1 binds to a transcriptional control element in Ty1

1993 ◽  
Vol 13 (1) ◽  
pp. 57-62
Author(s):  
B Errede
Keyword(s):  
Gene Expression ◽  
Transcriptional Control ◽  
Control Element ◽  
Regulatory Sequence ◽  
Gene Promoters ◽  
Adjacent Gene ◽  
Transposable Element Insertion ◽  
Transcriptional Regulatory ◽  
Transcriptional Regulatory Sequence ◽  
Insertion Mutations

Some Ty1 transposable-element insertion mutations of Saccharomyces cerevisiae activate adjacent-gene expression. These Ty1-activated genes are regulated similarly to certain mating genes. This report shows that the MCM1 protein, which binds to several mating genes, also binds to a transcriptional regulatory sequence in Ty1. The binding of MCM1 to Ty1 correlates with the ability of its binding site to function as a component of the Ty1 transcriptional activator. This correlation supports the idea that MCM1 is important for Ty1-activated gene expression. At mating-gene promoters, MCM1 binds with coactivators or repressors such as STE12, alpha 1, or alpha 2. In contrast, MCM1 binds without these associated DNA-binding proteins at its site in Ty1. This finding suggests that its role in Ty1-mediated transcription is different from that at mating genes.

scholarly journals Ancient duons may underpin spatial patterning of gene expression in C4 leaves

2018 ◽  
Vol 115 (8) ◽  
pp. 1931-1936 ◽  
Author(s):  
Ivan Reyna-Llorens ◽  
Steven J. Burgess ◽  
Gregory Reeves ◽  
Pallavi Singh ◽  
Sean R. Stevenson ◽  
...  
Keyword(s):  
Gene Expression ◽  
Regulatory Sequence ◽  
Spatial Patterning ◽  
Mesophyll Cells ◽  
Master Regulators ◽  
Transcriptional Regulatory ◽  
Bundle Sheath Cells ◽  
Photosynthesis Pathway ◽  
Photosynthesis Genes ◽  
Transcriptional Regulatory Sequence

If the highly efficient C4 photosynthesis pathway could be transferred to crops with the C3 pathway there could be yield gains of up to 50%. It has been proposed that the multiple metabolic and developmental modifications associated with C4 photosynthesis are underpinned by relatively few master regulators that have allowed the evolution of C4 photosynthesis more than 60 times in flowering plants. Here we identify a component of one such regulator that consists of a pair of cis-elements located in coding sequence of multiple genes that are preferentially expressed in bundle sheath cells of C4 leaves. These motifs represent duons as they play a dual role in coding for amino acids as well as controlling the spatial patterning of gene expression associated with the C4 leaf. They act to repress transcription of C4 photosynthesis genes in mesophyll cells. These duons are also present in the C3 model Arabidopsis thaliana, and, in fact, are conserved in all land plants and even some algae that use C3 photosynthesis. C4 photosynthesis therefore appears to have coopted an ancient regulatory code to generate the spatial patterning of gene expression that is a hallmark of C4 photosynthesis. This intragenic transcriptional regulatory sequence could be exploited in the engineering of efficient photosynthesis of crops.

scholarly journals Transcription factor IIIA gene expression in Xenopus oocytes utilizes a transcription factor similar to the major late transcription factor.

1989 ◽  
Vol 9 (11) ◽  
pp. 5003-5011 ◽  
Author(s):  
R K Hall ◽  
W L Taylor
Keyword(s):  
Gene Expression ◽  
Transcription Factor ◽  
Transcriptional Control ◽  
Somatic Cells ◽  
State Level ◽  
Control Element ◽  
Transcription Factor Iiia ◽  
Shift Analysis ◽  
Late Transcription ◽  
Upstream Element

Xenopus transcription factor IIIA (TFIIIA) gene expression is stringently regulated during development. The steady-state level of TFIIIA mRNA in a somatic cell is approximately 10(6) times less than in an immature oocyte. We have undertaken studies designed to identify differences in how the TFIIIA gene is transcribed in oocytes and somatic cells. In this regard, we have localized an upstream transcriptional control element in the TFIIIA promoter that stimulates transcription from the TFIIIA promoter approximately threefold in microinjected oocytes. The upstream element, in cis. does not stimulate transcription from the TFIIIA promoter in somatic cells. Thus, the element appears to be oocyte specific in the context of the TFIIIA promoter. However, both oocytes and somatic cells contain a protein (or a related protein) that binds the upstream element. We have termed this protein from oocytes the TFIIIA distal element factor. The sequence of the upstream element is similar to the sequence of the upstream element found in the adenovirus major late promoter that is a binding site for the major late transcription factor. By gel shift analysis, chemical footprinting, methylation intereference, and point mutation analysis, we demonstrate that the TFIIIA distal element factor and major late transcription factor have similar DNA-binding properties.

scholarly journals Comprehensive mapping of the human cytokine gene regulatory network

2020 ◽  
Author(s):  
CS Santoso ◽  
Z Li ◽  
S Lal ◽  
S Yuan ◽  
KA Gan ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transcriptional Control ◽  
Immune Cell ◽  
Cell Lineage ◽  
Cytokine Gene Expression ◽  
Gene Promoters ◽  
Cytokine Gene ◽  
Cytokine Regulation ◽  
Gene Regulatory ◽  
Human Cytokine

SummaryProper cytokine gene expression is essential in development, homeostasis, and immune responses. Studies on the transcriptional control of cytokine genes have mostly focused on highly researched transcription factors (TFs) and cytokines, resulting in an incomplete portrait of cytokine gene regulation. Here, we use enhanced yeast one-hybrid (eY1H) assays to derive a comprehensive network comprising 1,380 interactions between 265 TFs and 108 cytokine gene promoters, greatly expanding the known repertoire of TF-cytokine gene interactions. We found an enrichment of nuclear receptors and confirmed their role in cytokine regulation in primary macrophages. Additionally, we used the eY1H-derived network as a framework to identify pairs of TFs that synergistically modulate cytokine gene expression, and to identify novel TF-cytokine regulatory axes in immune diseases and immune cell lineage development. Overall, the eY1H data provides a rich resource to study cytokine regulation in a variety of physiological and disease contexts.

scholarly journals Putative cis-Acting Stem-Loops in the 5′ Untranslated Region of the Severe Acute Respiratory Syndrome Coronavirus Can Substitute for Their Mouse Hepatitis Virus Counterparts

2006 ◽  
Vol 80 (21) ◽  
pp. 10600-10614 ◽  
Author(s):  
Hyojeung Kang ◽  
Min Feng ◽  
Megan E. Schroeder ◽  
David P. Giedroc ◽  
Julian L. Leibowitz
Keyword(s):  
Severe Acute Respiratory Syndrome ◽  
Hepatitis Virus ◽  
Mouse Hepatitis Virus ◽  
Regulatory Sequence ◽  
Stem Loop ◽  
Cis Acting ◽  
Transcriptional Regulatory ◽  
Similar Phenotype ◽  
Transcriptional Regulatory Sequence

ABSTRACT Consensus covariation-based secondary structural models for the 5′ 140 nucleotides of the 5′ untranslated regions (5′UTRs) from mouse hepatitis virus (MHV) and severe acute respiratory syndrome coronavirus (SCoV) were developed and predicted three major helical stem-loop structures, designated stem-loop 1 (SL1), SL2, and SL4. The SCoV 5′UTR was predicted to contain a fourth stem-loop, named SL3, in which the leader transcriptional regulatory sequence (TRS) is folded into a hairpin loop. cDNAs corresponding to MHV/SCoV chimeric genomes were constructed by replacing the complete MHV 5′UTR with the corresponding SCoV sequence and by separately replacing MHV 5′UTR putative SL1, putative SL2, TRS, and putative SL4 with the corresponding SCoV sequences. Chimeric genomes were transcribed in vitro, and viruses were recovered after electroporation into permissive cells. Genomes in which the MHV 5′UTR SL1, SL2, and SL4 were individually replaced by their SCoV counterparts were viable. Chimeras containing the complete SCoV 5′UTR or the predicted SCoV SL3 were not viable. A chimera containing the SCoV 5′UTR in which the SCoV TRS was replaced with the MHV TRS was also not viable. The chimera containing the entire SCoV 5′UTR failed to direct the synthesis of any virus-specific RNA. Replacing the SCoV TRS with the MHV TRS in the MHV/5′UTR SCoV chimera permitted the synthesis of minus-sense genome-sized RNA but did not support the production of positive- or minus-sense subgenomic RNA7. A similar phenotype was obtained with the MHV/SCoV SL3 chimera. These results suggest a role for the TRS in the replication of minus-sense genomic RNA in addition to its known function in subgenomic RNA synthesis.

scholarly journals Transcriptional regulatory sequence divergence between mouse and bat modifies forelimb length

2008 ◽  
Vol 319 (2) ◽  
pp. 470
Author(s):  
Chris J. Cretekos ◽  
Ying Wang ◽  
Eric D. Green ◽  
James F. Martin ◽  
John J. Rasweiler ◽  
...  
Keyword(s):  
Sequence Divergence ◽  
Regulatory Sequence ◽  
Transcriptional Regulatory ◽  
Transcriptional Regulatory Sequence

Identification of the Promoter Region and the Transcriptional Regulatory Sequence of theevgASOperon ofEscherichia coli

1998 ◽  
Vol 62 (2) ◽  
pp. 286-290 ◽  
Author(s):  
Hiroyuki TANABE ◽  
Katsuhide YAMASAKI ◽  
Akinori KATOH ◽  
Sachiko YOSHIOKA ◽  
Ryutaro UTSUMI
Keyword(s):  
Promoter Region ◽  
Ofescherichia Coli ◽  
Regulatory Sequence ◽  
Transcriptional Regulatory ◽  
Transcriptional Regulatory Sequence

scholarly journals Modulation of the cytoplasmic functions of mammalian post-transcriptional regulatory proteins by methylation and acetylation: a key layer of regulation waiting to be uncovered?

2015 ◽  
Vol 43 (6) ◽  
pp. 1285-1295 ◽  
Author(s):  
Tajekesa K.P. Blee ◽  
Nicola K. Gray ◽  
Matthew Brook
Keyword(s):  
Gene Expression ◽  
Transcriptional Control ◽  
Regulatory Protein ◽  
Regulatory Proteins ◽  
Post Translational Modification ◽  
Control Of Gene Expression ◽  
Large Numbers ◽  
Transcriptional Regulatory ◽  
Mrna Fate ◽  
Normal Cellular Function

Post-transcriptional control of gene expression is critical for normal cellular function and viability and many of the proteins that mediate post-transcriptional control are themselves subject to regulation by post-translational modification (PTM), e.g. phosphorylation. However, proteome-wide studies are revealing new complexities in the PTM status of mammalian proteins, in particular large numbers of novel methylated and acetylated residues are being identified. Here we review studied examples of methylation/acetylation-dependent regulation of post-transcriptional regulatory protein (PTRP) function and present collated PTM data that points to the huge potential for regulation of mRNA fate by these PTMs.

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