scholarly journals Factors Influencing Broth Microdilution Antimicrobial Susceptibility Test Results for Dalbavancin, a New Glycopeptide Agent

2007 ◽  
Vol 45 (10) ◽  
pp. 3151-3154 ◽  
Author(s):  
R. P. Rennie ◽  
L. Koeth ◽  
R. N. Jones ◽  
T. R. Fritsche ◽  
C. C. Knapp ◽  
...  
Keyword(s):  
Antimicrobial Susceptibility ◽  
Susceptibility Test ◽  
Test Results ◽  
Broth Microdilution ◽  
Antimicrobial Susceptibility Test ◽  
Factors Influencing

scholarly journals Trends in antimicrobial resistance in Europe: update of EARSS results

2007 ◽  
Vol 12 (11) ◽  
Author(s):  
M De Kraker ◽  
N Van de Sande-Bruinsma
Keyword(s):  
Antimicrobial Resistance ◽  
Antimicrobial Susceptibility ◽  
Surveillance System ◽  
Bacterial Species ◽  
Susceptibility Test ◽  
Test Results ◽  
Antimicrobial Susceptibility Test ◽  
The Past ◽  
Antimicrobial Resistance Surveillance

For the past seven years (1999 to 2006), the European Antimicrobial Resistance Surveillance System (EARSS) has collected antimicrobial susceptibility test results of invasive isolates in humans of seven bacterial species that serve as indicators for the development of antimicrobial resistance in Europe.

scholarly journals A fast impedance-based antimicrobial susceptibility test

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Daniel C. Spencer ◽  
Teagan F. Paton ◽  
Kieran T. Mulroney ◽  
Timothy J. J. Inglis ◽  
J. Mark Sutton ◽  
...  
Keyword(s):  
Antimicrobial Susceptibility ◽  
Mode Of Action ◽  
Bacterial Species ◽  
Susceptibility Test ◽  
Electrical Characteristics ◽  
Label Free ◽  
Broth Microdilution ◽  
Antimicrobial Susceptibility Test ◽  
Antimicrobial Susceptibility Tests ◽  
Susceptibility Tests

Abstract There is an urgent need to develop simple and fast antimicrobial susceptibility tests (ASTs) that allow informed prescribing of antibiotics. Here, we describe a label-free AST that can deliver results within an hour, using an actively dividing culture as starting material. The bacteria are incubated in the presence of an antibiotic for 30 min, and then approximately 105 cells are analysed one-by-one with microfluidic impedance cytometry for 2–3 min. The measured electrical characteristics reflect the phenotypic response of the bacteria to the mode of action of a particular antibiotic, in a 30-minute incubation window. The results are consistent with those obtained by classical broth microdilution assays for a range of antibiotics and bacterial species.

scholarly journals Comparative Antimicrobial Susceptibility Profiles of Uropathogenic Extended-Spectrum ß-Lactamase Producing Strains of Klebsiella pneumonia and Escherichia coli by the CLSI and EUCAST Methodologies

2020 ◽  
Author(s):  
Doğan Akdoğan ◽  
Mustafa Güzel ◽  
Orhan Akpınar ◽  
Altan Aksoy ◽  
Yasemin Genç Bahçe
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Susceptibility ◽  
Susceptibility Test ◽  
Klebsiella Pneumonia ◽  
Test Results ◽  
Poor Agreement ◽  
Antimicrobial Susceptibility Test ◽  
E Coli ◽  
Extended Spectrum ◽  
Kappa Agreement

Aims: The lack of information about the inter variability of the test results obtained by CLSI and EUCAST requires further clarifications to interpret antimicrobial susceptibility patterns better. This study aimed to compare the CLSI and EUCAST interpretations of the antimicrobial susceptibility test results of the ESBL–producing uropathogenic Escherichia coli and Klebsiella pneumonia strains. Methods: After obtaining 157 ESBL-producing E. coli and 95, ESBL-producing K. pneumonia isolates from the urine specimens of the patients, Kirby-Bauer’s disc diffusion method was used for conducting antimicrobial susceptibility test. The test procedures and the interpretation of the results were carried out according to the year 2017 versions of both of the two guidelines. For the statistical comparison of concordance between the two guidelines, the Kappa coefficients and the concordance rates were calculated. Results: The results were graded in the range from perfect to poor agreement. For E. coli, interpretations of the AST results revealed a moderate to perfect agreement between both methods. Weighted Kappa agreement scores in the range from 0.42 to 1. The agreement for AMC, TPZ30/6, ceftazidime 10, meropenem, and aztreonam was poor without any inconsistencies. For Klebsiella, the kappa agreement score was in the range from 0.25 to 1. It was incompatible with AMC, TPZ 30/6, ceftazidime 10, aztreonam; there was poor agreement for cefepime, amikacin and ertapenem. Conclusions: Our results showed agreement between the two guidelines for uropathogenic extended-spectrum ß-lactamase producing Escherichia coli and Klebsiella pneumoniae but also showed inconsistencies between two guidelines. Therefore, the results of our study contribute to the comparison of these guidelines for interpreting antibiotic susceptibilities.

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