scholarly journals Assessment of Rapid Tests for Detection of Human Immunodeficiency Virus-Specific Antibodies in Recently Infected Individuals

2008 ◽  
Vol 46 (4) ◽  
pp. 1494-1497 ◽  
Author(s):  
B. Louie ◽  
E. Wong ◽  
J. D. Klausner ◽  
S. Liska ◽  
F. Hecht ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Specific Antibodies ◽  
Immunodeficiency Virus ◽  
Rapid Tests

scholarly journals Comparison of Indirect Immunofluorescence Assay as a Confirmatory Test to Detect Antibody to Human Immunodeficiency Virus with Line Imuune Assay and Western Blot Methods

2016 ◽  
Vol 2 (2) ◽  
pp. 12-16
Author(s):  
Ahmed Nawsher Alam ◽  
Mahmuda Siddiqua ◽  
Shahina Tabassum ◽  
Md Nazrul Islam
Keyword(s):  
Human Immunodeficiency Virus ◽  
Western Blot ◽  
Indirect Immunofluorescence ◽  
Immunofluorescence Assay ◽  
Indirect Immunofluorescence Assay ◽  
Confirmatory Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Immunodeficiency Virus ◽  
Positive Serum ◽  
Rapid Tests

In low prevalent countries of Human Immunodeficiency Virus (HIV) like Bangladesh, it is recommended that all HIVpositive sera tested by Enzyme Linked Immunosorbent Assay (ELISA) and/or rapid tests should be confirmed by Line Immune Assay (LIA) or Western Blot (WB) method. As these two tests are quite expensive, Indirect Immunofluorescence Assay (IFA) was evaluated as a confirmatory test and as an alternative to these two methods in the present study. A total of 92 subjects consisting of 46 HIV-antibody-positive patients and 46 controls were included in the study. All samples of sera were tested by ELISA and IFA methods, and some 34 of 46 HIV-antibody-positive samples were tested by LIA. One ELISA positive serum was found negative by both IFA and LIA. This result indicates that ELISA was 100% sensitive and 98.7% specific for detection of HIV antibody. Comparison between LIA with IFA on 34 samples, and WB with IFA on 26 samples showed 100% correlation between these methods. The study concluded that the IFA method is equal in performance as LIA and WB methods for the detection of antibody to HIV and can be used as a confirmatory test.

scholarly journals Diagnosis of Human Immunodeficiency Virus Type 1 Infection with Different Subtypes Using Rapid Tests

2000 ◽  
Vol 7 (4) ◽  
pp. 698-699 ◽  
Author(s):  
Susan Phillips ◽  
Timothy C. Granade ◽  
Chou-Pong Pau ◽  
Debra Candal ◽  
Dale J. Hu ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Sensitivity And Specificity ◽  
Human Immunodeficiency Virus Type ◽  
Virus Type ◽  
Local Population ◽  
Rapid Test ◽  
Immunodeficiency Virus ◽  
Rapid Tests ◽  
Hiv 1

ABSTRACT We evaluated six rapid tests for their sensitivity and specificity in diagnosing human immunodeficiency virus type 1 (HIV-1) infection using 241 specimens (172 HIV-1 positive, 69 HIV-1 negative) representing different HIV-1 subtypes (A [n = 40], B [n = 47], C [n = 28], E [n = 42], and F [n = 7]). HIVCHEK, Multispot, RTD and SeroStrip were 100% sensitive and specific. Capillus failed to identify two of eight subtype C specimens (overall sensitivity of 98.85%), while the SUDS test (the only test approved by the Food and Drug Administration) gave false-positive results for 5 of 69 seronegative specimens (specificity of 93.24%). Our results suggest that although rapid tests perform well in general, it may be prudent to evaluate a rapid test for sensitivity and specificity in a local population prior to its widespread use.

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