scholarly journals Evaluation and Implementation of a Chromogenic Agar Medium for Salmonella Detection in Stool in Routine Laboratory Diagnostics

2008 ◽  
Vol 47 (2) ◽  
pp. 456-458 ◽  
Author(s):  
S. van Dijk ◽  
M. J. Bruins ◽  
G. J. H. M. Ruijs
Infection ◽  
2006 ◽  
Vol 34 (2) ◽  
pp. 95-97 ◽  
Author(s):  
B. M. W. Diederen ◽  
C. M. van Leest ◽  
I. van Duijn ◽  
P. Willemse ◽  
P. H. J. van Keulen ◽  
...  

2013 ◽  
Vol 18 (2) ◽  
pp. 95-100
Author(s):  
MASAFUMI UCHIDA ◽  
HAJIME TERAMURA ◽  
MITSUAKI KASHIDA ◽  
HIDEMASA KODAKA

2018 ◽  
Vol 17 (2) ◽  
pp. 88-92
Author(s):  
I. V. Manina ◽  
V. Yu. Sergeev ◽  
N. V. Golubtsova ◽  
A. Yu. Sergeev

Introduction. Lymphocytes blast-transformation reaction (RBLT) is necessary for patient’s inspection with immunologic infringements. It is applied in the different fields of medicine to identification of a sensitization to antigens (allergens).Research objective – to modify and automate RBTL for application in routine laboratory practice.Materials and methods. RBTL, antigens (allergens). Software: Windows 7; Intel Pentium G4500 CPU. Digital CMOS video camera, Mikmed-6 microscope.Results. We analyzed difficulties when using RBTL in laboratory allergological practice. We created program files for automation of RBTL.Conclusion. We adapted and automated RBTL for laboratory diagnostics using.


2014 ◽  
Vol 53 (1) ◽  
pp. 105-112 ◽  
Author(s):  
Patricia J. Simner ◽  
Matthew W. Gilmour ◽  
Pat DeGagne ◽  
Kim Nichol ◽  
James A. Karlowsky

An efficient workflow to screen for and confirm the presence of carbapenemase-producing Gram-negative bacilli was developed by evaluating five chromogenic screening agar media and two confirmatory assays, the Rapid Carb screen test (Rosco Diagnostica A/S, Taastrup, Denmark) and the modified Hodge test. A panel of 150 isolates was used, including 49 carbapenemase-producing isolates representing a variety of β-lactamase enzyme classes. An evaluation of analytical performance, assay cost, and turnaround time indicated that the preferred workflow (screening test followed by confirmatory testing) was the chromID Carba agar medium (bioMérieux, Marcy l'Étoile, France), followed by the Rapid Carb screen test, yielding a combined sensitivity of 89.8% and a specificity of 100%. As an optional component of the workflow, a determination of carbapenemase gene class via molecular means could be performed subsequent to confirmatory testing.


2010 ◽  
Vol 51 (6) ◽  
pp. 704-706 ◽  
Author(s):  
R. Saito ◽  
S. Koyano ◽  
R. Nagai ◽  
N. Okamura ◽  
K. Moriya ◽  
...  

2010 ◽  
Vol 48 (4) ◽  
pp. 1510-1510 ◽  
Author(s):  
J. F. Peterson ◽  
K. M. Riebe ◽  
G. S. Hall ◽  
D. Wilson ◽  
S. Whittier ◽  
...  

2006 ◽  
Vol 43 (6) ◽  
pp. 615-618 ◽  
Author(s):  
J.D. Perry ◽  
M. Oliver ◽  
A. Nicholson ◽  
J. Wright ◽  
F.K. Gould

2018 ◽  
Vol 24 (2) ◽  
pp. 128-135
Author(s):  
S Gul Nahar ◽  
M Bulbul Hasan ◽  
Mst Rokeya Khatun ◽  
M Nawshad Ali

Objective: The present study was done to compare the performance of chromogenic agar medium and conventional culture media for the isolation and presumptive identification of uropathogen.Methodology: A total 300 sample were collected from Rajshahi Medical College Hospital, Bangladesh during January to June, 2008. Urine samples of the suspected UTI cases, showing pus cells >5/HPF on microscopic examination were included for urine culture simultaneously onto 2 conventional media (Blood agar and MacConkey agar) and chromogenic agar medium (HiCrome UTI agar medium). Results: Culture yielded 139 (46.33%) bacterial growth among them, 133 (44.33%) showed single organism and remaining 06 (2.00%) showed mixed growth of two organisms in different combinations. It is evident from the present study that both HiCrome UTI agar and Blood agar (BA) media supported growth of all 145 bacteria, while MacConkey (MAC) agar yielded 133(91.72%) bacterial growths. The rate of presumptive identification of the isolates was found significantly higher (97.24%) on HiCrome UTI agar when compared with the MacConkey agar (80.68%) and Blood agar (27.58%) media. Out of 91 E. coli isolated, 88(96.70%) could be identified differentially on HiCrome UTI agar medium in contrast to 85(93.40%) on MacConkey agar and only 06(06.59%) on Blood agar. Again, all 06 (100%) of the isolate-pairs of mixed growth were identified distinctly on HiCrome UTI agar, whereas both Blood agar and MacConkey agar media could revealed only 01(16.66%) of the polymicrobial growth.Conclusion: HiCrome UTI agar medium has been documented for its very high yielding rate, rapid presumptive identification of both single and polymicrobial growths with greater precision and avoidance of biochemical tests for further identification of uropathogens. Thus it can be recommended as primary urine culture medium to be used by the clinical microbiology laboratories.TAJ 2011; 24(2): 128-135


Food Control ◽  
2002 ◽  
Vol 13 (6-7) ◽  
pp. 377-379 ◽  
Author(s):  
Y Hara-Kudo ◽  
M Ikedo ◽  
O Komatsu ◽  
S Yamamoto ◽  
S Kumagai

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