scholarly journals Collection and Characterization of Samples for Establishment of a Serum Repository for Lyme Disease Diagnostic Test Development and Evaluation

2014 ◽  
Vol 52 (10) ◽  
pp. 3755-3762 ◽  
Author(s):  
C. R. Molins ◽  
C. Sexton ◽  
J. W. Young ◽  
L. V. Ashton ◽  
R. Pappert ◽  
...  
Keyword(s):  
Lyme Disease ◽  
Diagnostic Test ◽  
Test Development

New ultrastructural findings about Borrelia burgdorferi by cryofixation, negative staining, thin sectioning and scanning techniques

1990 ◽  
Vol 48 (3) ◽  
pp. 582-583
Author(s):  
S. F. Hayes ◽  
M. D. Corwin ◽  
T. G. Schwan ◽  
D. W. Dorward ◽  
W. Burgdorfer
Keyword(s):  
Lyme Disease ◽  
Borrelia Burgdorferi ◽  
Structural Characterization ◽  
Negative Staining ◽  
Low Speed ◽  
Thin Sectioning ◽  
Ultrastructural Findings

Characterization of Borrelia burgdorferi strains by means of negative staining EM has become an integral part of many studies related to the biology of the Lyme disease organism. However, relying solely upon negative staining to compare new isolates with prototype B31 or other borreliae is often unsatisfactory. To obtain more satisfactory results, we have relied upon a correlative approach encompassing a variety EM techniques, i.e., scanning for topographical features and cryotomy, negative staining and thin sectioning to provide a more complete structural characterization of B. burgdorferi.For characterization, isolates of B. burgdorferi were cultured in BSK II media from which they were removed by low speed centrifugation. The sedimented borrelia were carefully resuspended in stabilizing buffer so as to preserve their features for scanning and negative staining. Alternatively, others were prepared for conventional thin sectioning and for cryotomy using modified procedures. For thin sectioning, the fixative described by Ito, et al.

Use-Conditions-Based Board Level Shock Test Development for Handheld Components

2004 ◽  
Author(s):  
Shubhada Sahasrabudhe ◽  
Vinayak Pandey ◽  
Betty Phillips ◽  
Kang Joon Lee ◽  
Lei Mercado
Keyword(s):  
Solder Joint ◽  
Cell Phone ◽  
Standardized Test ◽  
Test Development ◽  
Failure Mechanisms ◽  
Reliability Test ◽  
Shock Test ◽  
Board Level ◽  
Test Repeatability

For handheld electronic applications such as cell phones and Personal Digital Assistants (PDAs), drop/impact could result in considerable flexure of the printed circuit board (PCB) mounted inside the cell phone housing. The mechanical stresses may cause electrical failure of the components, with typical failure mechanisms of board trace cracking, solder joint fatigue, and solder pad cracking. A standardized test needs to be developed to assess reliability of handheld components subjected to impacts. The test should facilitate high volume testing, maximize margin for safety factors, and capture the failure mechanisms in the field environment. To develop the reliability test using use conditions based reliability methodology, comprehensive characterization of the mechanical field stresses during end use conditions is particularly essential. This paper discusses complete cell phone drop characterization along with the shock test developed to test the components subjected to such drops. Novel fixtures have been designed to simulate free fall of the cell phone in specific orientations. After the complete characterization of cell phone use conditions, board level shock test has been selected to assess component reliability. Test repeatability, number of components on the test board, and layout of the components are some of the factors considered during the board level shock test development. Several parameters like screw and washer designs, torque have been studied to yield excellent test repeatability. Nonlinear Dynamic Finite Element Simulation has been performed to provide more insight into the interaction of the bending modes and its impact on the solder joint failures. This paper demonstrates the process of understanding use conditions, developing reliability tests, validating test results and driving industry standards.

scholarly journals Characterization of outer membranes isolated from Borrelia burgdorferi, the Lyme disease spirochete.

1995 ◽  
Vol 63 (6) ◽  
pp. 2154-2163 ◽  
Author(s):  
J D Radolf ◽  
M S Goldberg ◽  
K Bourell ◽  
S I Baker ◽  
J D Jones ◽  
...  
Keyword(s):  
Lyme Disease ◽  
Borrelia Burgdorferi ◽  
Outer Membranes

scholarly journals Implementation of the US Department of Health and Human Services Zika Specimen Repository and Its Effect on Zika Diagnostic Test Development, 2016

2019 ◽  
Vol 134 (2_suppl) ◽  
pp. 53S-57S ◽  
Author(s):  
Marla Petway ◽  
Laura Anderson ◽  
Rosemary Humes ◽  
Stephanie Sincock ◽  
Wendi Kuhnert-Tallman ◽  
...  
Keyword(s):  
Diagnostic Test ◽  
Zika Virus ◽  
Human Services ◽  
Clinical Sample ◽  
Test Development ◽  
Disease Outbreaks ◽  
Clinical Samples ◽  
Molecular Testing ◽  
Health And Human Services ◽  
Department Of Health

This study describes the efforts and outcomes associated with the establishment of a clinical sample repository during the 2016 Zika virus epidemic. To overcome the challenge of limited access to clinical samples to support diagnostic test development, multiple US Department of Health and Human Services (HHS) agencies formed a partnership to create the HHS Zika Specimen Repository. In 2016-2017, the Biomedical Advanced Research and Development Authority and the Centers for Disease Control and Prevention collected patient specimens (4420 convalescent sera aliquots from 100 donors and 7171 plasma aliquots from 239 donors), confirmed Zika virus test results, assembled 1 panel for molecular testing (n = 25 sets) and 7 panels for serologic testing (n = 92), and distributed the panels to test developers. We manufactured 8 test panels and distributed 74 sets of panels to 32 commercial companies, public health partners, and research institutions. Manufacturers used these panels to generate data that supported 14 US Food and Drug Administration (FDA) emergency use authorizations and 1 FDA approval. To develop a repository that can respond immediately to future disease outbreaks, we recommend that organizations pre-position procedures, resources, and partnerships to optimize each partner’s contribution.

scholarly journals Characterization of 5′-methylthioadenosine/S-adenosylhomocysteine nucleosidases from Borrelia burgdorferi: Antibiotic targets for Lyme disease

2020 ◽  
Vol 1864 (1) ◽  
pp. 129455 ◽  
Author(s):  
Kenneth A. Cornell ◽  
Reece J. Knippel ◽  
Gerald R. Cortright ◽  
Meghan Fonken ◽  
Christian Guerrero ◽  
...  
Keyword(s):  
Lyme Disease ◽  
Borrelia Burgdorferi
Sign in / Sign up

Export Citation Format

Share Document