scholarly journals Global Genomic Analysis of Pseudomonas savastanoi pv. savastanoi Plasmids

2007 ◽  
Vol 190 (2) ◽  
pp. 625-635 ◽  
Author(s):  
Isabel Pérez-Martínez ◽  
Youfu Zhao ◽  
Jesús Murillo ◽  
George W. Sundin ◽  
Cayo Ramos
Keyword(s):  
Pseudomonas Syringae ◽  
Insertion Sequence ◽  
Genomic Analysis ◽  
Secretion System ◽  
Type Iv Secretion ◽  
Gene Content ◽  
Type Iv ◽  
Lack Of Information ◽  
Genes Encoding ◽  
Indole 3 Acetic Acid

ABSTRACT Pseudomonas savastanoi pv. savastanoi strains harbor native plasmids belonging to the pPT23A plasmid family (PFPs) which are detected in all pathovars of the related species Pseudomonas syringae examined and contribute to the ecological and pathogenic fitness of their host. However, there is a general lack of information about the gene content of P. savastanoi pv. savastanoi plasmids and their role in the interaction of this pathogen with olive plants. We designed a DNA macroarray containing 135 plasmid-borne P. syringae genes to conduct a global genetic analysis of 32 plasmids obtained from 10 P. savastanoi pv. savastanoi strains. Hybridization results revealed that the number of PFPs per strain varied from one to four. Additionally, most strains contained at least one plasmid (designated non-PFP) that did not hybridize to the repA gene of pPT23A. Only three PFPs contained genes involved in the biosynthesis of the virulence factor indole-3-acetic acid (iaaM, iaaH, and iaaL). In contrast, ptz, a gene involved in the biosynthesis of cytokinins, was found in five PFPs and one non-PFP. Genes encoding a type IV secretion system (T4SS), type IVA, were found in both PFPs and non-PFPs; however, type IVB genes were found only on PFPs. Nine plasmids encoded both T4SSs, whereas seven other plasmids carried none of these genes. Most PFPs and non-PFPs hybridized to at least one putative type III secretion system effector gene and to a variety of additional genes encoding known P. syringae virulence factors and one or more insertion sequence transposase genes. These results indicate that non-PFPs may contribute to the virulence and fitness of the P. savastanoi pv. savastanoi host. The overall gene content of P. savastanoi pv. savastanoi plasmids, with their repeated information, mosaic arrangement, and insertion sequences, suggests a possible role in adaptation to a changing environment.

scholarly journals Comparative Genomic Analysis of the pPT23A Plasmid Family of Pseudomonas syringae

2005 ◽  
Vol 187 (6) ◽  
pp. 2113-2126 ◽  
Author(s):  
Youfu Zhao ◽  
Zhonghua Ma ◽  
George W. Sundin
Keyword(s):  
Pseudomonas Syringae ◽  
Bacterial Pathogen ◽  
Genomic Analysis ◽  
Complete Sequence ◽  
Secretion System ◽  
Comparative Genomic ◽  
Type Iv ◽  
Gene Acquisition ◽  
Genes Encoding ◽  
Type Ivb Secretion

ABSTRACT Members of the pPT23A plasmid family of Pseudomonas syringae play an important role in the interaction of this bacterial pathogen with host plants. Complete sequence analysis of several pPT23A family plasmids (PFPs) has provided a glimpse of the gene content and virulence function of these plasmids. We constructed a macroarray containing 161 genes to estimate and compare the gene contents of 23 newly analyzed and eight known PFPs from 12 pathovars of P. syringae, which belong to four genomospecies. Hybridization results revealed that PFPs could be distinguished by the type IV secretion system (T4SS) encoded and separated into four groups. Twelve PFPs along with pPSR1 from P. syringae pv. syringae, pPh1448B from P. syringae pv. phaseolicola, and pPMA4326A from P. syringae pv. maculicola encoded a type IVA T4SS (VirB-VirD4 conjugative system), whereas 10 PFPs along with pDC3000A and pDC3000B from P. syringae pv. tomato encoded a type IVB T4SS (tra system). Two plasmids encoded both T4SSs, whereas six other plasmids carried none or only a few genes of either the type IVA or type IVB secretion system. Most PFPs hybridized to more than one putative type III secretion system effector gene and to a variety of additional genes encoding known P. syringae virulence factors. The overall gene contents of individual PFPs were more similar among plasmids within each of the four groups based on T4SS genes; however, a number of genes, encoding plasmid-specific functions or hypothetical proteins, were shared among plasmids from different T4SS groups. The only gene shared by all PFPs in this study was the repA gene, which encoded sequences with 87 to 99% amino acid identityamong 25 sequences examined. We proposed a model to illustrate the evolution and gene acquisition of the pPT23A plasmid family. To our knowledge, this is the first such attempt to conduct a global genetic analysis of this important plasmid family.

scholarly journals Protein Transfer through an F Plasmid-Encoded Type IV Secretion System Suppresses the Mating-Induced SOS Response

mBio ◽  
2021 ◽  
Author(s):  
Abu Amar M. Al Mamun ◽  
Kouhei Kishida ◽  
Peter J. Christie
Keyword(s):  
Heavy Metals ◽  
Sos Response ◽  
Secretion System ◽  
Type Iv Secretion ◽  
Type Iv Secretion System ◽  
Conjugative Transfer ◽  
Secretion Systems ◽  
Protein Transfer ◽  
Type Iv ◽  
Genes Encoding

Many bacteria carry plasmids and other mobile genetic elements (MGEs) whose conjugative transfer through encoded type IV secretion systems (T4SSs), or “mating channels,” can lead to a rapid intra- and interspecies proliferation of genes encoding resistance to antibiotics or heavy metals or virulence traits. Here, we show that a model IncF plasmid-encoded T4SS translocates not only DNA but also several proteins intercellularly.

scholarly journals Ecological Genomics of Marine Roseobacters

2007 ◽  
Vol 73 (14) ◽  
pp. 4559-4569 ◽  
Author(s):  
M. A. Moran ◽  
R. Belas ◽  
M. A. Schell ◽  
J. M. González ◽  
F. Sun ◽  
...  
Keyword(s):  
Dynamic Environment ◽  
Secretion System ◽  
Type Iv Secretion ◽  
Type Iv Secretion System ◽  
Ecological Genomics ◽  
Type Iv ◽  
Aromatic Compound Degradation ◽  
Number Of Genes ◽  
Genes Encoding ◽  
Diverse Interactions

ABSTRACT Bacterioplankton of the marine Roseobacter clade have genomes that reflect a dynamic environment and diverse interactions with marine plankton. Comparative genome sequence analysis of three cultured representatives suggests that cellular requirements for nitrogen are largely provided by regenerated ammonium and organic compounds (polyamines, allophanate, and urea), while typical sources of carbon include amino acids, glyoxylate, and aromatic metabolites. An unexpectedly large number of genes are predicted to encode proteins involved in the production, degradation, and efflux of toxins and metabolites. A mechanism likely involved in cell-to-cell DNA or protein transfer was also discovered: vir-related genes encoding a type IV secretion system typical of bacterial pathogens. These suggest a potential for interacting with neighboring cells and impacting the routing of organic matter into the microbial loop. Genes shared among the three roseobacters and also common in nine draft Roseobacter genomes include those for carbon monoxide oxidation, dimethylsulfoniopropionate demethylation, and aromatic compound degradation. Genes shared with other cultured marine bacteria include those for utilizing sodium gradients, transport and metabolism of sulfate, and osmoregulation.

Identification of the virB operon genes encoding the type IV secretion system, in Colombian Brucella canis isolates

2013 ◽  
Vol 163 (1-2) ◽  
pp. 196-199 ◽  
Author(s):  
Juan Jacobo de la Cuesta-Zuluaga ◽  
Miryan Margot Sánchez-Jiménez ◽  
Juliana Martínez-Garro ◽  
Martha Olivera-Angel
Keyword(s):  
Secretion System ◽  
Type Iv Secretion ◽  
Type Iv Secretion System ◽  
Type Iv ◽  
Brucella Canis ◽  
Genes Encoding ◽  
Virb Operon

scholarly journals Nucleotide Sequence and Evolution of the Five-Plasmid Complement of the Phytopathogen Pseudomonas syringae pv. maculicola ES4326

2004 ◽  
Vol 186 (15) ◽  
pp. 5101-5115 ◽  
Author(s):  
John Stavrinides ◽  
David S. Guttman
Keyword(s):  
Nucleotide Sequence ◽  
Pseudomonas Syringae ◽  
Genomic Analysis ◽  
Type Iv Secretion ◽  
Comparative Genomic ◽  
Human Pathogens ◽  
Plasmid Evolution ◽  
Type Iv ◽  
Wide Range ◽  
Genomic Analyses

ABSTRACT Plasmids are transmissible, extrachromosomal genetic elements that are often responsible for environmental or host-specific adaptations. In order to identify the forces driving the evolution of these important molecules, we determined the complete nucleotide sequence of the five-plasmid complement of the radish and Arabidopsis pathogen Pseudomonas syringae pv. maculicola ES4326 and conducted an intraspecific comparative genomic analysis. To date, this is the most complex fully sequenced plasmid complement of any gram-negative bacterium. The plasmid complement comprises two pPT23A-like replicons, pPMA4326A (46,697 bp) and pPMA4326B (40,110 bp); a pPS10-like replicon, pPMA4326C (8,244 bp); and two atypical, replicase-deficient replicons, pPMA4326D (4,833 bp) and pPMA4326E (4,217 bp). A complete type IV secretion system is found on pPMA4326A, while the type III secreted effector hopPmaA is present on pPMA4326B. The region around hopPmaA includes a shorter hopPmaA homolog, insertion sequence (IS) elements, and a three-element cassette composed of a resolvase, an integrase, and an exeA gene that is also present in several human pathogens. We have also identified a novel genetic element (E622) that is present on all but the smallest plasmid (pPMA4326E) that has features of an IS element but lacks an identifiable transposase. This element is associated with virulence-related genes found in a wide range of P. syringae strains. Comparative genomic analyses of these and other P. syringae plasmids suggest a role for recombination and integrative elements in driving plasmid evolution.

scholarly journals The Coxiella burnetii Cryptic Plasmid Is Enriched in Genes Encoding Type IV Secretion System Substrates

2011 ◽  
Vol 193 (7) ◽  
pp. 1493-1503 ◽  
Author(s):  
D. E. Voth ◽  
P. A. Beare ◽  
D. Howe ◽  
U. M. Sharma ◽  
G. Samoilis ◽  
...  
Keyword(s):  
Coxiella Burnetii ◽  
Secretion System ◽  
Type Iv Secretion ◽  
Cryptic Plasmid ◽  
Type Iv Secretion System ◽  
Type Iv ◽  
Genes Encoding

scholarly journals Genes for the Type IV Secretion System in an Intracellular Symbiont, Wolbachia, a Causative Agent of Various Sexual Alterations in Arthropods

2000 ◽  
Vol 182 (22) ◽  
pp. 6529-6531 ◽  
Author(s):  
Shinji Masui ◽  
Tetsuhiko Sasaki ◽  
Hajime Ishikawa
Keyword(s):  
Causative Agent ◽  
Pathogenic Bacteria ◽  
Secretion System ◽  
Type Iv Secretion ◽  
Type Iv Secretion System ◽  
Intracellular Bacteria ◽  
Intracellular Symbiont ◽  
Type Iv ◽  
Essential Components ◽  
Genes Encoding

ABSTRACT Wolbachia species are intracellular bacteria known to cause reproductive abnormalities in their hosts. In this study, we identified Wolbachia genes encoding homologs to the type IV secretion system by which many pathogenic bacteria secrete macromolecules. The genes identified encoded most of the essential components of the secretion system and were cotranscribed as an operon.

High prevalence of functional type IV secretion system for CagA protein in Japanese clinical Helicobacter pylori

2001 ◽  
Vol 120 (5) ◽  
pp. A652-A653
Author(s):  
Y HIRATA ◽  
S MAEDA ◽  
Y MITUNO ◽  
M AKANUMA ◽  
T KAWABE ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Secretion System ◽  
Type Iv Secretion ◽  
Type Iv Secretion System ◽  
Functional Type ◽  
Caga Protein ◽  
Type Iv ◽  
High Prevalence
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