scholarly journals Regulation of the Chlamydia trachomatis Histone H1-Like Protein Hc2 Is IspE Dependent and IhtA Independent

2006 ◽  
Vol 188 (14) ◽  
pp. 5289-5292 ◽  
Author(s):  
Nicole A. Grieshaber ◽  
Janet Burgess Sager ◽  
Cheryl A. Dooley ◽  
Stanley F. Hayes ◽  
Ted Hackstadt
Keyword(s):  
Gene Expression ◽  
Chlamydia Trachomatis ◽  
Chromatin Structure ◽  
Histone H1 ◽  
Regulatory Rna ◽  
Developmentally Regulated ◽  
Global Regulators ◽  
Small Regulatory Rna ◽  
Expression And Activity

ABSTRACT The chlamydial histone-like proteins, Hc1 and Hc2, function as global regulators of chromatin structure and gene expression. Hc1 and Hc2 expression and activity are developmentally regulated. A small metabolite that disrupts Hc1 interaction with DNA also disrupts Hc2 interactions; however, the small regulatory RNA that inhibits Hc1 translation is specific to Hc1.

scholarly journals LuxS-dependent AI-2 production is not involved in global regulation of natural product biosynthesis inPhotorhabdusandXenorhabdus

PeerJ ◽  
2017 ◽  
Vol 5 ◽  
pp. e3471 ◽  
Author(s):  
Antje K. Heinrich ◽  
Merle Hirschmann ◽  
Nick Neubacher ◽  
Helge B. Bode
Keyword(s):  
Regulatory System ◽  
Regulatory Rna ◽  
Insect Larvae ◽  
Phenotypic Differences ◽  
Global Regulators ◽  
Autoinducer 2 ◽  
Small Regulatory Rna ◽  
Mutant Strains ◽  
Different Strains

The Gram-negative bacteriaPhotorhabdusandXenorhabdusare known to produce a variety of different natural products (NP). These compounds play different roles since the bacteria live in symbiosis with nematodes and are pathogenic to insect larvae in the soil. Thus, a fine tuned regulatory system controlling NP biosynthesis is indispensable. Global regulators such as Hfq, Lrp, LeuO and HexA have been shown to influence NP production ofPhotorhabdusandXenorhabdus. Additionally, photopyrones as quorum sensing (QS) signals were demonstrated to be involved in the regulation of NP production inPhotorhabdus.In this study, we investigated the role of another possible QS signal, autoinducer-2 (AI-2), in regulation of NP production. The AI-2 synthase (LuxS) is widely distributed within the bacterial kingdom and has a dual role as a part of the activated methyl cycle pathway, as well as being responsible for AI-2 precursor production. We deletedluxSin three different entomopathogenic bacteria and compared NP levels in the mutant strains to the wild type (WT) but observed no difference to the WT strains. Furthermore, the absence of the small regulatory RNAmicA, which is encoded directly upstream ofluxS, did not influence NP levels. Phenotypic differences between theP. luminescens luxSdeletion mutant and an earlier describedluxSdeficient strain ofP. luminescenssuggested that two phenotypically different strains have evolved in different laboratories.

Expanded synthetic small regulatory RNA expression platforms for rapid and multiplex gene expression knockdown

2019 ◽  
Vol 54 ◽  
pp. 180-190 ◽  
Author(s):  
Dongsoo Yang ◽  
Seung Min Yoo ◽  
Changdai Gu ◽  
Jae Yong Ryu ◽  
Jae Eun Lee ◽  
...  
Keyword(s):  
Gene Expression ◽  
Rna Expression ◽  
Regulatory Rna ◽  
Small Regulatory Rna

scholarly journals sRNA-mediated activation of gene expression by inhibition of 5'-3’ exonucleolytic mRNA degradation

eLife ◽  
2017 ◽  
Vol 6 ◽  
Author(s):  
Sylvain Durand ◽  
Frédérique Braun ◽  
Anne-Catherine Helfer ◽  
Pascale Romby ◽  
Ciarán Condon
Keyword(s):  
Gene Expression ◽  
Transcriptional Control ◽  
Mrna Degradation ◽  
Regulatory Rna ◽  
Primary Transcript ◽  
Ribosome Binding ◽  
Small Regulatory Rna ◽  
Adaptive Processes ◽  
Open Question ◽  
Malate Transporter

Post-transcriptional control by small regulatory RNA (sRNA) is critical for rapid adaptive processes. sRNAs can directly modulate mRNA degradation in Proteobacteria without interfering with translation. However, Firmicutes have a fundamentally different set of ribonucleases for mRNA degradation and whether sRNAs can regulate the activity of these enzymes is an open question. We show that Bacillus subtilis RoxS, a major trans-acting sRNA shared with Staphylococus aureus, prevents degradation of the yflS mRNA, encoding a malate transporter. In the presence of malate, RoxS transiently escapes from repression by the NADH-sensitive transcription factor Rex and binds to the extreme 5’-end of yflS mRNA. This impairs the 5’-3’ exoribonuclease activity of RNase J1, increasing the half-life of the primary transcript and concomitantly enhancing ribosome binding to increase expression of the transporter. Globally, the different targets regulated by RoxS suggest that it helps readjust the cellular NAD+/NADH balance when perturbed by different stimuli.

Small Regulatory Molecules Acting Big in Cancer: Potential Role of Mito-miRs in Cancer

2019 ◽  
Vol 19 (9) ◽  
pp. 621-631 ◽  
Author(s):  
Praveen Sharma ◽  
Bharat ◽  
Nilambra Dogra ◽  
Sandeep Singh
Keyword(s):  
Gene Expression ◽  
Mitochondrial Genome ◽  
Potential Role ◽  
Regulatory Rna ◽  
Rna Molecules ◽  
Small Regulatory Rna ◽  
Physiological Processes ◽  
Current Review ◽  
Posttranscriptional Level

MicroRNAs [miRNAs] are short, non-coding, single stranded RNA molecules regulating gene expression of their targets at the posttranscriptional level by either degrading mRNA or by inhibiting translation. Previously, miRNAs have been reported to be present inside the mitochondria and these miRNAs have been termed as mito-miRs. Origin of these mito-miRs may either be from mitochondrial genome or import from nucleus. The second class of mito-miRs makes it important to unravel the involvement of miRNAs in crosstalk between nucleus and mitochondria. Since miRNAs are involved in various physiological processes, their deregulation is often associated with disease progression, including cancer. The current review focuses on the involvement of miRNAs in different mitochondrial mediated processes. It also highlights the importance of exploring the interaction of miRNAs with mitochondrial genome, which may lead to the development of small regulatory RNA based therapeutic options.

Identification of Differentially Expressed Genes Using cDNA-AFLP During Six Days In Vitro Tuberization of Potato

Zuriat ◽  
2015 ◽  
Vol 14 (1) ◽  
Author(s):  
Nono Carsono ◽  
Christian Bachem
Keyword(s):  
Gene Expression ◽  
Developmental Process ◽  
Expression Patterns ◽  
Induction Medium ◽  
In Vitro Tuberization ◽  
Storage Organ ◽  
Developmentally Regulated ◽  
Study Gene Expression ◽  
Differential Pattern

Tuberization in potato is a complex developmental process resulting in the differentiation of stolon into the storage organ, tuber. During tuberization, change in gene expression has been known to occur. To study gene expression during tuberization over the time, in vitro tuberization system provides a suitable tool, due to its synchronous in tuber formation. An early six days axillary bud growing on tuber induction medium is a crucial development since a large number of genes change in their expression patterns during this period. In order to identify, isolate and sequencing the genes which displaying differential pattern between tuberizing and non-tuberizing potato explants during six days in vitro tuberization, cDNA-AFLP fingerprint, method for the visualization of gene expression using cDNA as template which is amplified to generate an RNA-fingerprinting, was used in this experiment. Seventeen primer combinations were chosen based on their expression profile from cDNA-AFLP fingerprint. Forty five TDFs (transcript derived fragment), which displayed differential expressions, were obtained. Tuberizing explants had much more TDFs, which developmentally regulated, than those from non tuberizing explants. Seven TDFs were isolated, cloned and then sequenced. One TDF did not find similarity in the current databases. The nucleotide sequence of TDF F showed best similarity to invertase ezymes from the databases. The homology of six TDFs with known sequences is discussed in this paper.

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