ATP Synthesis by Oxidative Phosphorylation

EcoSal Plus ◽  
2007 ◽  
Vol 2 (2) ◽  
Author(s):  
Steven B. Vik
Keyword(s):  
Oxidative Phosphorylation ◽  
Atp Synthesis

On the mechanism of ATP synthesis in oxidative phosphorylation: A review

1974 ◽  
Vol 3 (1) ◽  
pp. 1-15 ◽  
Author(s):  
John H. Young ◽  
Ephraim F. Korman ◽  
Jerome McLick
Keyword(s):  
Oxidative Phosphorylation ◽  
Atp Synthesis

scholarly journals The ADP/ATP Carrier and Its Relationship to Oxidative Phosphorylation in Ancestral Protist Trypanosoma brucei

2015 ◽  
Vol 14 (3) ◽  
pp. 297-310 ◽  
Author(s):  
Anna Gnipová ◽  
Karolína Šubrtová ◽  
Brian Panicucci ◽  
Anton Horváth ◽  
Julius Lukeš ◽  
...  
Keyword(s):  
Oxidative Phosphorylation ◽  
Trypanosoma Brucei ◽  
Atp Synthesis ◽  
Model Organisms ◽  
Growth Defect ◽  
Respiratory Chain Complexes ◽  
Content Type ◽  
Chain Complexes ◽  
Parasitic Protist ◽  
Veterinary Importance

ABSTRACT The highly conserved ADP/ATP carrier (AAC) is a key energetic link between the mitochondrial (mt) and cytosolic compartments of all aerobic eukaryotic cells, as it exchanges the ATP generated inside the organelle for the cytosolic ADP. Trypanosoma brucei , a parasitic protist of medical and veterinary importance, possesses a single functional AAC protein (TbAAC) that is related to the human and yeast ADP/ATP carriers. However, unlike previous studies performed with these model organisms, this study showed that TbAAC is most likely not a stable component of either the respiratory supercomplex III+IV or the ATP synthasome but rather functions as a physically separate entity in this highly diverged eukaryote. Therefore, TbAAC RNA interference (RNAi) ablation in the insect stage of T. brucei does not impair the activity or arrangement of the respiratory chain complexes. Nevertheless, RNAi silencing of TbAAC caused a severe growth defect that coincides with a significant reduction of mt ATP synthesis by both substrate and oxidative phosphorylation. Furthermore, TbAAC downregulation resulted in a decreased level of cytosolic ATP, a higher mt membrane potential, an elevated amount of reactive oxygen species, and a reduced consumption of oxygen in the mitochondria. Interestingly, while TbAAC has previously been demonstrated to serve as the sole ADP/ATP carrier for ADP influx into the mitochondria, our data suggest that a second carrier for ATP influx may be present and active in the T. brucei mitochondrion. Overall, this study provides more insight into the delicate balance of the functional relationship between TbAAC and the oxidative phosphorylation (OXPHOS) pathway in an early diverged eukaryote.

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