scholarly journals The Iron-Sulfur Cluster Proteins Isa1 and Isa2 Are Required for the Function but Not for the De Novo Synthesis of the Fe/S Clusters of Biotin Synthase in Saccharomyces cerevisiae

2007 ◽  
Vol 6 (4) ◽  
pp. 753-753 ◽  
Author(s):  
Ulrich Mühlenhoff ◽  
Mathias J. Gerl ◽  
Birgit Flauger ◽  
Heike M. Pirner ◽  
Sandra Balser ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
De Novo ◽  
De Novo Synthesis ◽  
Biotin Synthase ◽  
Iron Sulfur Cluster ◽  
Sulfur Cluster ◽  
Iron Sulfur

scholarly journals The Iron-Sulfur Cluster Proteins Isa1 and Isa2 Are Required for the Function but Not for the De Novo Synthesis of the Fe/S Clusters of Biotin Synthase in Saccharomyces cerevisiae

2007 ◽  
Vol 6 (3) ◽  
pp. 495-504 ◽  
Author(s):  
Ulrich Mühlenhoff ◽  
Mathias J. Gerl ◽  
Birgit Flauger ◽  
Heike M. Pirner ◽  
Sandra Balser ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
De Novo ◽  
Mitochondrial Protein ◽  
Cluster Assembly ◽  
De Novo Synthesis ◽  
Biotin Synthase ◽  
Iron Sulfur Cluster ◽  
Iron Sulfur ◽  
Low Levels

ABSTRACT The yeast Saccharomyces cerevisiae is able to use some biotin precursors for biotin biosynthesis. Insertion of a sulfur atom into desthiobiotin, the final step in the biosynthetic pathway, is catalyzed by biotin synthase (Bio2). This mitochondrial protein contains two iron-sulfur (Fe/S) clusters that catalyze the reaction and are thought to act as a sulfur donor. To identify new components of biotin metabolism, we performed a genetic screen and found that Isa2, a mitochondrial protein involved in the formation of Fe/S proteins, is necessary for the conversion of desthiobiotin to biotin. Depletion of Isa2 or the related Isa1, however, did not prevent the de novo synthesis of any of the two Fe/S centers of Bio2. In contrast, Fe/S cluster assembly on Bio2 strongly depended on the Isu1 and Isu2 proteins. Both isa mutants contained low levels of Bio2. This phenotype was also found in other mutants impaired in mitochondrial Fe/S protein assembly and in wild-type cells grown under iron limitation. Low Bio2 levels, however, did not cause the inability of isa mutants to utilize desthiobiotin, since this defect was not cured by overexpression of BIO2. Thus, the Isa proteins are crucial for the in vivo function of biotin synthase but not for the de novo synthesis of its Fe/S clusters. Our data demonstrate that the Isa proteins are essential for the catalytic activity of Bio2 in vivo.

Iron–sulfur cluster dynamics in biotin synthase: A new [2Fe–2S]1+ cluster

2009 ◽  
Vol 381 (4) ◽  
pp. 487-490 ◽  
Author(s):  
Manuela Lotierzo ◽  
Bernadette Tse Sum Bui ◽  
Helen K. Leech ◽  
Martin J. Warren ◽  
Andrée Marquet ◽  
...  
Keyword(s):  
Biotin Synthase ◽  
Iron Sulfur Cluster ◽  
Sulfur Cluster ◽  
Iron Sulfur

scholarly journals Mutagenesis of the proposed iron-sulfur cluster binding ligands in Escherichia coli biotin synthase

FEBS Letters ◽  
2000 ◽  
Vol 466 (2-3) ◽  
pp. 372-376 ◽  
Author(s):  
Kirsty S Hewitson ◽  
Jack E Baldwin ◽  
Nicholas M Shaw ◽  
Peter L Roach
Keyword(s):  
Escherichia Coli ◽  
Biotin Synthase ◽  
Iron Sulfur Cluster ◽  
Sulfur Cluster ◽  
Iron Sulfur

scholarly journals Identification of IscU residues critical for de novo iron–sulfur cluster assembly

2019 ◽  
Vol 112 (6) ◽  
pp. 1769-1783
Author(s):  
Naoyuki Tanaka ◽  
Eiki Yuda ◽  
Takashi Fujishiro ◽  
Kei Hirabayashi ◽  
Kei Wada ◽  
...  
Keyword(s):  
De Novo ◽  
Cluster Assembly ◽  
Iron Sulfur Cluster ◽  
Sulfur Cluster ◽  
Iron Sulfur

scholarly journals Cytosolic HSC20 integrates de novo iron–sulfur cluster biogenesis with the CIAO1-mediated transfer to recipients

2018 ◽  
Vol 27 (5) ◽  
pp. 837-852 ◽  
Author(s):  
Ki Soon Kim ◽  
Nunziata Maio ◽  
Anamika Singh ◽  
Tracey A Rouault
Keyword(s):  
De Novo ◽  
Iron Sulfur Cluster ◽  
Sulfur Cluster ◽  
Iron Sulfur

scholarly journals A Complex between Biotin Synthase and the Iron−Sulfur Cluster Assembly Chaperone HscA That Enhances in Vivo Cluster Assembly

Biochemistry ◽  
2009 ◽  
Vol 48 (45) ◽  
pp. 10782-10792 ◽  
Author(s):  
Michael R. Reyda ◽  
Corey J. Fugate ◽  
Joseph T. Jarrett
Keyword(s):  
Cluster Assembly ◽  
Biotin Synthase ◽  
Iron Sulfur Cluster ◽  
Sulfur Cluster ◽  
Iron Sulfur

Saccharomyces cerevisiae ISU1 and ISU2: members of a well-conserved gene family for iron-sulfur cluster assembly

1999 ◽  
Vol 294 (4) ◽  
pp. 897-907 ◽  
Author(s):  
Stacey A Garland ◽  
Kevin Hoff ◽  
Larry E Vickery ◽  
Valeria Cizewski Culotta
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Gene Family ◽  
Cluster Assembly ◽  
Iron Sulfur Cluster ◽  
Sulfur Cluster ◽  
Iron Sulfur ◽  
Conserved Gene
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