scholarly journals Probiotic Bacteria May Become Dormant during Storage

2005 ◽  
Vol 71 (3) ◽  
pp. 1662-1663 ◽  
Author(s):  
Sampo J. Lahtinen ◽  
Miguel Gueimonde ◽  
Arthur C. Ouwehand ◽  
Johanna P. Reinikainen ◽  
Seppo J. Salminen
Keyword(s):  
Cell Membrane ◽  
Clinical Significance ◽  
Probiotic Bacteria ◽  
Bacterial Viability ◽  
Viable Cells ◽  
Nonculturable Cells

ABSTRACT The determination of bacterial viability in probiotic products is of economic, technological, and clinical significance. We compared four methods to enumerate three Bifidobacterium strains in fermented oat products during storage. A subpopulation of nonculturable cells retained a functional cell membrane typical of viable cells, indicating that probiotic bacteria become dormant during storage.

Non-contact-based determination of membrane permeability to water and dimethyl sulfoxide of cells virtually trapped in a self-induced micro-vortex

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Hsiu-Yang Tseng ◽  
Chiu-Jen Chen ◽  
Zong-Lin Wu ◽  
Yong-Ming Ye ◽  
Guo-Zhen Huang
Keyword(s):  
Cell Membrane ◽  
Dimethyl Sulfoxide ◽  
Membrane Permeability ◽  
Cell Membrane Permeability ◽  
Cell Type ◽  
Cryoprotective Agents ◽  
A Cell

Cell-membrane permeability to water (Lp) and cryoprotective agents (Ps) of a cell type is a crucial cellular information for achieving optimal cryopreservation in the biobanking industry. In this work, a...

scholarly journals CLINICAL SIGNIFICANCE OF DETERMINATION OF IGE ANTIBODIES TO STAPHYLOCOCCUS AUREUS AND MALASSEZIA SPECIES IN SERUM OF CHILDREN WITH ATOPIC DERMATITIS

2018 ◽  
Vol 63 (4) ◽  
pp. 84-88
Author(s):  
T. S. Okuneva ◽  
M. V. Kushnareva ◽  
A. N. Pampura ◽  
E. E. Varlamov ◽  
N. G. Konyukova
Keyword(s):  
Staphylococcus Aureus ◽  
Atopic Dermatitis ◽  
Clinical Significance ◽  
Specific Ige ◽  
Frequency Of Occurrence ◽  
Ige Antibodies ◽  
Malassezia Species ◽  
Specific Ige Antibodies ◽  
Malassezia Spp

We studied the frequency of occurrence and concentration of allergen-specific IgE antibodies (asIgE) to enterotoxins A, B, C and TSST-1 of Staphylococcus aureus and Malassezia spp. in the serum of children with atopic dermatitis in the acute period of the disease. The determination of asIgE levels was performed in 84 children aged from 5 months to 17 years using the immunochemiluminescent method on the UniCAP 100 analyzer (Phadia, Sweden). The level of asIgE was evaluated on a scale of 6 classes.The sensitization to S. aureus enterotoxins was detected in 29 children with аsIgE concentration from 0.35 to 25.8 kU/L (I–IV classes). In 16 children were observed 8 polysensitization options to S. aureus allergens. Combinations of asIgE to A + B and A + B + C enterotoxins were most common – in every fourth and fifth child,respectively. Sensitization to Malassezia spp. was observed in 41 children with concentration from 0.38 to 98.9 KU/L (I–V classes). Polysensitization to both S. aureus and Malassezia spp. allergens was observed in one of two children. A higher degree of sensitisation to Malassezia spp allergens has been established compared to S. aureus.Conclusion. To improve the specific diagnostics of atopic dermatitis, it is advisable to examine the serum for a complete panel of recombinant S. aureus enterotoxins in combination with the determination of asIgE to Malassezia spp. 

scholarly journals Determination of Probiotic Bacteria from Intestine of Sparisoma viride and Bioencapsulation of Artemia salina with Probionts

2019 ◽  
Vol 15 (1) ◽  
pp. 1-8
Author(s):  
R. Muthezhila ◽  
N. Yogananth ◽  
M. Akila ◽  
M. Syed Ali ◽  
M. Jayaprakas
Keyword(s):  
Artemia Salina ◽  
Probiotic Bacteria

scholarly journals Application of Flow Cytometry to Segregated Kinetic Modeling Based on the Physiological States of Microorganisms

2007 ◽  
Vol 73 (12) ◽  
pp. 3993-4000 ◽  
Author(s):  
Covadonga Quir�s ◽  
M�nica Herrero ◽  
Luis A. Garc�a ◽  
Mario D�az
Keyword(s):  
Flow Cytometry ◽  
Culture Media ◽  
Glucose Consumption ◽  
Viable Cell ◽  
Counting Method ◽  
Batch Cultures ◽  
Viable Cells ◽  
The Status ◽  
Standard Culture

ABSTRACT Flow cytometry (FC) has been introduced to characterize and to assess the physiological states of microorganisms in conjunction with the classical plate-counting method. To show the applicability of the technique, in particular for the development of kinetic models, pure culture fermentation experiments were followed over time, using both prokaryotic (Lactobacillus hilgardii) and eukaryotic (Saccharomyces cerevisiae) microorganisms growing in standard culture media (MRS and YPD). The differences observed between the active and viable cells determined by FC and CFU, respectively, allowed us to determine that a large number of cells were in a viable but nonculturable (VBNC) state, which resulted in a subpopulation much larger than the damaged-cell (double-stained) subpopulation. Finally, the determination of the evolution of viable, the VBNC, and the dead cells allowed us to develop a segregated kinetic model to describe the yeast and the bacteria population dynamics and glucose consumption in batch cultures. This model, more complete than that which is traditionally used, based only on viable cell measurements, describes better the behavior and the functionality of the cultures, giving a deeper knowledge in real time about the status and the course of the bioprocesses.

Determination of Total, Viable Cells and Enterobacteraceae in Categorized Milk Powder

2009 ◽  
Vol 8 (9) ◽  
pp. 1493-1496
Author(s):  
Imran Rashid Raj ◽  
M. Khaskheli ◽  
H.A. Kaleri ◽  
S. A. Fazlani ◽  
K. Devi ◽  
...  
Keyword(s):  
Milk Powder ◽  
Viable Cells

Hydraulische Leitfähigkeit von Valonia utricularis / Hydraulic Conductivity of Valonia utricularis

1971 ◽  
Vol 26 (12) ◽  
pp. 1302-1311 ◽  
Author(s):  
E. Steudle ◽  
U. Zimmermann
Keyword(s):  
Hydraulic Conductivity ◽  
Cell Membrane ◽  
Marine Alga ◽  
Water Movement ◽  
Turgor Pressure ◽  
Irreversible Thermodynamics ◽  
Rapid Changes ◽  
Cell Turgor ◽  
Valonia Utricularis

A method is described for the simultaneous determination of rapid changes of the cell turgor pressure (hydrostatic pressure) in algal cells (cell size must be at least 3 mm in diameter), and of the net volume flow across the cell membrane arising after a change of the cell turgor pressure or of the osmotic pressure in the outside medium. On the basis of the equations of irreversible thermodynamics it is possible to calculate the hydraulic conductivity of the cell membrane from these measurements, as it is theoretically shown.The hydraulic conductivities of the marine alga Valonia utricularis determined in two independent ways (by osmotic and hydrostatic experiments) are equal. For exosmosis, Lpex (hydrostatic) and Lpex (osmotic) amounted to (9,6 ± 1,0) ·10-7 and (9,8 ± 1,9) · 10-7 respectively cm · sec-1 · atm-1, and for endomosis, Lpen (hydrostatic) was (9,4 ± 1,1) ·10-7 cm · sec-1 · atm-1.A polarity in the water movement across the cell membranes as discussed in the literature could not be found for Valonia utricularis.

Sign in / Sign up

Export Citation Format

Share Document