scholarly journals Community Dynamics and Activity of Ammonia-Oxidizing Prokaryotes in Intertidal Sediments of the Yangtze Estuary

2013 ◽  
Vol 80 (1) ◽  
pp. 408-419 ◽  
Author(s):  
Yanling Zheng ◽  
Lijun Hou ◽  
Silvia Newell ◽  
Min Liu ◽  
Junliang Zhou ◽  
...  
Keyword(s):  
Community Dynamics ◽  
Yangtze Estuary ◽  
Estuarine Sediments ◽  
Ammonia Oxidizing Bacteria ◽  
Intertidal Sediments ◽  
The Yangtze Estuary ◽  
Α Subunit ◽  
Unifrac Distance ◽  
Gene Abundance ◽  
Potential Nitrification

ABSTRACTDiversity, abundance, and activity of ammonia-oxidizing bacteria (AOB) and ammonia-oxidizing archaea (AOA) were investigated using the ammonia monooxygenase α subunit (amoA) in the intertidal sediments of the Yangtze Estuary. Generally, AOB had a lower diversity ofamoAgenes than did AOA in this study. Clone library analysis revealed great spatial variations in both AOB and AOA communities along the estuary. The UniFrac distance matrix showed that all the AOB communities and 6 out of 7 AOA communities in the Yangtze Estuary were statistically indistinguishable between summer and winter. The studied AOB and AOA community structures were observed to correlate with environmental parameters, of which salinity, pH, ammonium, total phosphorus, and organic carbon had significant correlations with the composition and distribution of both communities. Also, the AOA communities were significantly correlated with sediment clay content. Quantitative PCR (qPCR) results indicated that the abundance of AOBamoAgenes was greater than that of AOAamoAgenes in 10 of the 14 samples analyzed in this study. Potential nitrification rates were significantly greater in summer than in winter and had a significant negative correlation with salinity. In addition, potential nitrification rates were correlated strongly only with archaealamoAgene abundance and not with bacterialamoAgene abundance. However, no significant differences were observed between rates measured with and without ampicillin (AOB inhibitor). These results implied that archaea might play a more important role in mediating the oxidation of ammonia to nitrite in the Yangtze estuarine sediments.

The effects of semi-lunar spring and neap tidal change on nutrients cycling in the intertidal sediments of the Yangtze estuary

2005 ◽  
Vol 48 (2) ◽  
pp. 255-264 ◽  
Author(s):  
L. J. Hou ◽  
M. Liu ◽  
S. Y. Xu ◽  
D. N. Ou ◽  
J. J. Lu ◽  
...  
Keyword(s):  
Yangtze Estuary ◽  
Intertidal Sediments ◽  
The Yangtze Estuary ◽  
Nutrients Cycling ◽  
Tidal Change

DNRA in intertidal sediments of the Yangtze Estuary

2017 ◽  
Vol 122 (8) ◽  
pp. 1988-1998 ◽  
Author(s):  
Guoyu Yin ◽  
Lijun Hou ◽  
Min Liu ◽  
Xiaofei Li ◽  
Yanling Zheng ◽  
...  
Keyword(s):  
Yangtze Estuary ◽  
Intertidal Sediments ◽  
The Yangtze Estuary

Distribution and accumulation of biogenic silica in the intertidal sediments of the Yangtze Estuary

2008 ◽  
Vol 20 (5) ◽  
pp. 543-550 ◽  
Author(s):  
Lijun HOU ◽  
Min LIU ◽  
Shiyuan XU ◽  
Huimin YAN ◽  
Dongni OU ◽  
...  
Keyword(s):  
Biogenic Silica ◽  
Yangtze Estuary ◽  
Intertidal Sediments ◽  
The Yangtze Estuary

scholarly journals amoAGene Abundances and Nitrification Potential Rates Suggest that Benthic Ammonia-Oxidizing Bacteria and Not Archaea Dominate N Cycling in the Colne Estuary, United Kingdom

2014 ◽  
Vol 81 (1) ◽  
pp. 159-165 ◽  
Author(s):  
Jialin Li ◽  
David B. Nedwell ◽  
Jessica Beddow ◽  
Alex J. Dumbrell ◽  
Boyd A. McKew ◽  
...  
Keyword(s):  
Denaturing Gradient Gel Electrophoresis ◽  
Dry Weight ◽  
Estuarine Sediments ◽  
Ammonia Oxidizer ◽  
Ammonia Oxidizing Bacteria ◽  
Content Type ◽  
Gene Abundance ◽  
Nitrification Potential ◽  
Gradient Gel Electrophoresis ◽  
Colne Estuary

ABSTRACTNitrification, mediated by ammonia-oxidizing bacteria (AOB) and ammonia-oxidizing archaea (AOA), is important in global nitrogen cycling. In estuaries where gradients of salinity and ammonia concentrations occur, there may be differential selections for ammonia-oxidizer populations. The aim of this study was to examine the activity, abundance, and diversity of AOA and AOB in surface oxic sediments of a highly nutrified estuary that exhibits gradients of salinity and ammonium. AOB and AOA communities were investigated by measuring ammonia monooxygenase (amoA) gene abundance and nitrification potentials both spatially and temporally. Nitrification potentials differed along the estuary and over time, with the greatest nitrification potentials occurring mid-estuary (8.2 μmol N grams dry weight [gdw]−1day−1in June, increasing to 37.4 μmol N gdw−1day−1in January). At the estuary head, the nitrification potential was 4.3 μmol N gdw−1day−1in June, increasing to 11.7 μmol N gdw−1day−1in January. At the estuary head and mouth, nitrification potentials fluctuated throughout the year. AOBamoAgene abundances were significantly greater (by 100-fold) than those of AOA both spatially and temporally.Nitrosomonasspp. were detected along the estuary by denaturing gradient gel electrophoresis (DGGE) band sequence analysis. In conclusion, AOB dominated over AOA in the estuarine sediments, with the ratio of AOB/AOAamoAgene abundance increasing from the upper (freshwater) to lower (marine) regions of the Colne estuary. These findings suggest that in this nutrified estuary, AOB (possiblyNitrosomonasspp.) were of major significance in nitrification.

Phosphorus speciation and availability in intertidal sediments of the Yangtze Estuary, China

2009 ◽  
Vol 24 (1) ◽  
pp. 120-128 ◽  
Author(s):  
L.J. Hou ◽  
M. Liu ◽  
Y. Yang ◽  
D.N. Ou ◽  
X. Lin ◽  
...  
Keyword(s):  
Yangtze Estuary ◽  
Intertidal Sediments ◽  
Phosphorus Speciation ◽  
The Yangtze Estuary

Diversity, Abundance, and Distribution of nirS-Harboring Denitrifiers in Intertidal Sediments of the Yangtze Estuary

2015 ◽  
Vol 70 (1) ◽  
pp. 30-40 ◽  
Author(s):  
Yanling Zheng ◽  
Lijun Hou ◽  
Min Liu ◽  
Juan Gao ◽  
Guoyu Yin ◽  
...  
Keyword(s):  
Yangtze Estuary ◽  
Intertidal Sediments ◽  
The Yangtze Estuary ◽  
Abundance And Distribution
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