scholarly journals Thiols in Nitric Oxide Synthase-Containing Nocardia sp. Strain NRRL 5646

2007 ◽  
Vol 73 (9) ◽  
pp. 3095-3097 ◽  
Author(s):  
Sungwon Lee ◽  
Hélène Bergeron ◽  
Peter C. K. Lau ◽  
John P. N. Rosazza
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Dehydrogenase Activity ◽  
Formaldehyde Dehydrogenase ◽  
Nocardia Brasiliensis ◽  
Cell Extracts ◽  
Nocardia Sp ◽  
Oxide Synthase ◽  
Nitric Oxide Toxicity

ABSTRACT Mycothiol (MSH) [1-d-myo-inosityl-2-(N-acetyl-l-cysteinyl)amido-2-deoxy-α-d-glucopyranoside], isolated as the bimane derivative, was established to be the major thiol in Nocardia sp. strain NRRL 5646, a species most closely related to Nocardia brasiliensis strain DSM 43758T. Thiol formation and detection of MSH-dependent formaldehyde dehydrogenase activity in cell extracts are relevant to the possible modulation of nitric oxide toxicity generated by strain NRRL 5646.

scholarly journals Inducible nitric oxide synthase blockade with aminoguanidine, protects mice infected with Nocardia brasiliensis from actinomycetoma development

2020 ◽  
Vol 14 (10) ◽  
pp. e0008775
Author(s):  
Mario C. Salinas-Carmona ◽  
Ossian Longoria-Lozano ◽  
Humberto R. Garza-Esquivel ◽  
Juan López-Ulloa ◽  
Jorge Reyes-Carrillo ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Inducible Nitric Oxide Synthase ◽  
Nocardia Brasiliensis ◽  
Oxide Synthase ◽  
Inducible Nitric Oxide

scholarly journals Cyclic Guanosine-3′,5′-Monophosphate and Biopteridine Biosynthesis in Nocardia sp

2000 ◽  
Vol 182 (13) ◽  
pp. 3644-3648 ◽  
Author(s):  
Jong-Keun Son ◽  
John P. N. Rosazza
Keyword(s):  
Nitric Oxide ◽  
Guanylate Cyclase ◽  
Culture Media ◽  
Enzyme System ◽  
The Other ◽  
Cell Extracts ◽  
Nocardia Sp ◽  
Average Control ◽  
Nos Inhibitor ◽  
Oxide Synthase

ABSTRACT Nocardia sp. strain NRRL 5646 contains a nitric oxide synthase (NOS) enzyme system capable of generating nitric oxide (NO) from arginine and arginine-containing peptides. To explain possible roles of the NOS system in this bacterium, guanylate cyclase (GC) and tetrahydrobiopterin (H4B) biosynthetic enzymes were identified in cell extracts and in culture media. Cell extracts contained GC activity, as measured by the conversion of GTP to cyclic guanosine-3′,5′-monophosphate (cGMP) at 9.56 pmol of cGMP h−1 mg of protein−1. Concentrations of extracellular cGMP in culture media were significantly increased, from average control levels of 45 pmol cGMP liter−1 to a maximum of 315 pmol liter−1, in response to additions of GTP, l-arginine, H4B, and sodium nitroprusside to growing Nocardia cultures. On the other hand, the NOS inhibitor N G-nitro-l-arginine and the GC inhibitor 1H-[1,2,4]oxadiazole[4,3-a]quinoxalin-1-one both dramatically decreased extracellular cGMP levels. Activities for GTP-cyclohydrase-1,6-pyruvoyltetrahydropterin synthase and sepiapterin reductase, enzymes essential for H4B biosynthesis, were present in Nocardia culture extracts at 77.5 pmol of neopterin and 45.8 pmol of biopterin h−1 mg of protein−1, respectively. In Nocardia spp., as in mammals, GTP is a key intermediate in H4B biosynthesis, and GTP is converted to cGMP by a GC enzyme system that is activated by NO.

Localization of endothelial nitric oxide synthase in the normal and failing human atrial myocardium

1996 ◽  
Vol 54 ◽  
pp. 786-787
Author(s):  
Chi-Ming Wei ◽  
Margarita Bracamonte ◽  
Shi-Wen Jiang ◽  
Richard C. Daly ◽  
Christopher G.A. McGregor ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Heart Failure ◽  
Nitric Oxide Synthase ◽  
Endothelial Nitric Oxide Synthase ◽  
Cardiac Tissues ◽  
Mammalian Tissues ◽  
End Stage Heart Failure ◽  
End Stage ◽  
Oxide Synthase ◽  
Endothelial Nitric Oxide

Nitric oxide (NO) is a potent endothelium-derived relaxing factor which also may modulate cardiomyocyte inotropism and growth via increasing cGMP. While endothelial nitric oxide synthase (eNOS) isoforms have been detected in non-human mammalian tissues, expression and localization of eNOS in the normal and failing human myocardium are poorly defined. Therefore, the present study was designed to investigate eNOS in human cardiac tissues in the presence and absence of congestive heart failure (CHF).Normal and failing atrial tissue were obtained from six cardiac donors and six end-stage heart failure patients undergoing primary cardiac transplantation. ENOS protein expression and localization was investigated utilizing Western blot analysis and immunohistochemical staining with the polyclonal rabbit antibody to eNOS (Transduction Laboratories, Lexington, Kentucky).

Nitric oxide synthase and cellac disease

2001 ◽  
Vol 120 (5) ◽  
pp. A684-A684
Author(s):  
I DANIELS ◽  
I MURRAY ◽  
W GODDARD ◽  
R LONG
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Oxide Synthase

Inhibitory effect of melatonin on nitric oxide synthase in rat enteric nervous system

2001 ◽  
Vol 120 (5) ◽  
pp. A176-A176
Author(s):  
P KOPPITZ ◽  
M STORR ◽  
D SAUR ◽  
M KURJAK ◽  
H ALLESCHER
Keyword(s):  
Nitric Oxide ◽  
Nervous System ◽  
Nitric Oxide Synthase ◽  
Enteric Nervous System ◽  
Inhibitory Effect ◽  
Oxide Synthase
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