scholarly journals Comparison of Aerobic Methanotrophic Communities in Littoral and Profundal Sediments of Lake Constance by a Molecular Approach

2007 ◽  
Vol 73 (13) ◽  
pp. 4389-4394 ◽  
Author(s):  
Monali Rahalkar ◽  
Bernhard Schink
Keyword(s):  
16S Rrna ◽  
Lake Constance ◽  
Gene Clone ◽  
Rrna Gene ◽  
Type I ◽  
Methanotrophic Bacteria ◽  
Molecular Approach ◽  
Clone Libraries ◽  
Methanotrophic Communities ◽  
Prominent Peak

ABSTRACT Analysis of pmoA and 16S rRNA gene clone libraries of methanotrophic bacteria in Lake Constance revealed an overall dominance of type I methanotrophs in both littoral and profundal sediments. The sediments exhibited minor differences in their methanotrophic community structures. Type X methanotrophs made up a significant part of the clone libraries only in the profundal sediment and were also found only there as a prominent peak by T-RFLP analyses.

Methylohalobius crimeensis gen. nov., sp. nov., a moderately halophilic, methanotrophic bacterium isolated from hypersaline lakes of Crimea

2005 ◽  
Vol 55 (5) ◽  
pp. 1817-1826 ◽  
Author(s):  
Jürgen Heyer ◽  
Ursula Berger ◽  
Martin Hardt ◽  
Peter F. Dunfield
Keyword(s):  
Fatty Acids ◽  
16S Rrna ◽  
Gene Sequence ◽  
Rrna Gene ◽  
Type I ◽  
Methanotrophic Bacteria ◽  
Rrna Gene Sequence ◽  
Methanotrophic Bacterium ◽  
Hypersaline Lakes ◽  
Novel Genus And Species

A novel genus and species are proposed for two strains of methanotrophic bacteria isolated from hypersaline lakes in the Crimean Peninsula of Ukraine. Strains 10KiT and 4Kr are moderate halophiles that grow optimally at 1–1·5 M (5·8–8·7 %, w/v) NaCl and tolerate NaCl concentrations from 0·2 M up to 2·5 M (1·2–15 %). This optimum and upper limit are the highest for any methanotrophic bacterium known to date. The strains are Gram-negative, aerobic, non-pigmented, motile, coccoid to spindle-shaped bacteria that grow on methane or methanol only and utilize the ribulose monophosphate pathway for carbon assimilation. They are neutrophilic (growth occurs only in the range pH 6·5–7·5) and mesophilic (optimum growth occurs at 30 °C). On the basis of 16S rRNA gene sequence phylogeny, strains 10KiT and 4Kr represent a type I methanotroph within the ‘Gammaproteobacteria’. However, the 16S rRNA gene sequence displays <91·5 % identity to any public-domain sequence. The most closely related methanotrophic bacterium is the thermophilic strain HB. The DNA G+C content is 58·7 mol%. The major phospholipid fatty acids are 18 : 1ω7 (52–61 %), 16 : 0 (22–23 %) and 16 : 1ω7 (14–20 %). The dominance of 18 : 1 over 16 : 0 and 16 : 1 fatty acids is unique among known type I methanotrophs. The data suggest that strains 10KiT and 4Kr should be considered as belonging to a novel genus and species of type I methanotrophic bacteria, for which the name Methylohalobius crimeensis gen. nov., sp. nov. is proposed. Strain 10KiT (=DSM 16011T=ATCC BAA-967T) is the type strain.

scholarly journals Characterization of the microbial community in different types of Daqu samples as revealed by 16S rRNA and 26S rRNA gene clone libraries

2014 ◽  
Vol 31 (1) ◽  
pp. 199-208 ◽  
Author(s):  
Xiao-Wei Zheng ◽  
Zheng Yan ◽  
M. J. Robert Nout ◽  
Teun Boekhout ◽  
Bei-Zhong Han ◽  
...  
Keyword(s):  
Microbial Community ◽  
16S Rrna ◽  
Gene Clone ◽  
Rrna Gene ◽  
Clone Libraries ◽  
Different Types ◽  
26S Rrna

Bacterial, archaeal and eukaryotic diversity in Arctic sediment as revealed by 16S rRNA and 18S rRNA gene clone libraries analysis

Polar Biology ◽  
2008 ◽  
Vol 32 (1) ◽  
pp. 93-103 ◽  
Author(s):  
Fei Tian ◽  
Yong Yu ◽  
Bo Chen ◽  
Huirong Li ◽  
Yu-Feng Yao ◽  
...  
Keyword(s):  
16S Rrna ◽  
18S Rrna ◽  
18S Rrna Gene ◽  
Gene Clone ◽  
Rrna Gene ◽  
Clone Libraries ◽  
Eukaryotic Diversity ◽  
Arctic Sediment

The Effect of Protozoa on the Composition of Rumen Bacteria in Cattle Using 16S rRNA Gene Clone Libraries

2005 ◽  
Vol 69 (3) ◽  
pp. 499-506 ◽  
Author(s):  
Yuhei OZUTSUMI ◽  
Kiyoshi TAJIMA ◽  
Akio TAKENAKA ◽  
Hisao ITABASHI
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Gene Clone ◽  
Rrna Gene ◽  
Rumen Bacteria ◽  
Clone Libraries
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