scholarly journals Pigmentiphaga sp. Strain D-2 Uses a Novel Amidase To Initiate the Catabolism of the Neonicotinoid Insecticide Acetamiprid

2020 ◽  
Vol 86 (6) ◽  
Author(s):  
Hongxing Yang ◽  
Shunli Hu ◽  
Xiang Wang ◽  
Shaochuang Chuang ◽  
Weibin Jia ◽  
...  
Keyword(s):  
Molecular Mechanisms ◽  
Nitrile Hydratase ◽  
Environmental Fate ◽  
Draft Genome ◽  
The Novel ◽  
Content Type ◽  
Neonicotinoid Insecticide ◽  
Α And Β Subunits ◽  
Hydrolysis Of ◽  
Considerable Concern

ABSTRACT Acetamiprid, a chloronicotinyl neonicotinoid insecticide, is among the most commonly used insecticides worldwide, and its environmental fate has caused considerable concern. The compound 1-(6-chloropyridin-3-yl)-N-methylmethanamine (IM 1-4) has been reported to be the main intermediate during acetamiprid catabolism in microorganisms, honeybees, and spinach. However, the molecular mechanism underlying the hydrolysis of acetamiprid to IM 1-4 has not yet been elucidated. In this study, a novel amidase (AceAB) that initially hydrolyzes the C-N bond of acetamiprid to generate IM 1-4 was purified and characterized from the acetamiprid-degrading strain Pigmentiphaga sp. strain D-2. Based on peptide profiling of the purified AceAB and the draft genome sequence of strain D-2, aceA (372 bp) and aceB (2,295 bp), encoding the α and β subunits of AceAB, respectively, were cloned and found to be necessary for acetamiprid hydrolysis in strain D-2. The characteristics of AceAB were also systematically investigated. Though AceA and AceB showed 35% to 56% identity to the α and β subunits of the N,N-dimethylformamidase from Paracoccus aminophilus, AceAB was specific for the hydrolysis of acetamiprid and showed no activities to N,N-dimethylformamide or its structural analogs. IMPORTANCE Acetamiprid, among the top neonicotinoid insecticides used worldwide, is one of the most important commercial insecticides. Due to its extensive use, the environmental fate of acetamiprid, especially its microbial degradation, has caused considerable concern. Although the catabolic pathways of acetamiprid in microorganisms have been extensively studied, the molecular mechanisms underlying acetamiprid biodegradation (except for a nitrile hydratase) remain largely unknown, and the enzyme responsible for the biotransformation of acetamiprid into its main intermediate, IM 1-4, have not yet been elucidated. The amidase AceAB and its encoding genes, aceA and aceB, characterized in this study, were found to be necessary and specific for the initial hydrolysis of the C-N bond of acetamiprid to generate IM 1-4 in Pigmentiphaga sp. strain D-2. The finding of the novel amidase AceAB will greatly enhance our understanding of the microbial catabolism of the widely used insecticide acetamiprid at the molecular level.

scholarly journals Draft Genome Sequence of Microbacterium esteraromaticum MM1, a Bacterium That Hydrolyzes the Organophosphorus Pesticide Fenamiphos, Isolated from Golf Course Soil

2018 ◽  
Vol 7 (4) ◽  
Author(s):  
Logeshwaran Panneerselvan ◽  
Kannan Krishnan ◽  
Suresh R. Subashchandrabose ◽  
Ravi Naidu ◽  
Mallavarapu Megharaj
Keyword(s):  
Polycyclic Aromatic Hydrocarbon ◽  
Genome Sequence ◽  
Draft Genome ◽  
Organophosphorus Pesticide ◽  
South Australia ◽  
Draft Genome Sequence ◽  
Golf Course ◽  
Content Type ◽  
Polycyclic Aromatic ◽  
Hydrolysis Of

In this study, we report the first draft genome sequence of Microbacterium esteraromaticum MM1, isolated from golf course soil in South Australia. The genome possesses genes for the hydrolysis of organophosphorus (OP) pesticides and polycyclic aromatic hydrocarbon (PAH) degradation.

scholarly journals Draft Genome Sequence of Lactobacillus rhamnosus OSU-PECh-69, a Cheese Isolate with Antibacterial Activity

2020 ◽  
Vol 9 (37) ◽  
Author(s):  
Israel García-Cano ◽  
Walaa E. Hussein ◽  
Diana Rocha-Mendoza ◽  
Ahmed E. Yousef ◽  
Rafael Jiménez-Flores
Keyword(s):  
Genome Sequence ◽  
Antimicrobial Agents ◽  
Draft Genome ◽  
Lactobacillus Rhamnosus ◽  
Gc Content ◽  
Gene Clusters ◽  
Gram Negative Bacteria ◽  
The Novel ◽  
Content Type ◽  
A Genome

ABSTRACT The novel strain Lactobacillus rhamnosus OSU-PECh-69 was isolated from provolone cheese. It produces antimicrobial agents having a molecular mass of 5 to 10 kDa that are active against Gram-positive and Gram-negative bacteria. The strain has a genome sequence of 3,057,669 bp, a GC content of 46.6%, and up to two gene clusters encoding bacteriocins.

scholarly journals Draft Genome Sequence of the Novel Enterobacter cloacae Strain amazonensis, a Highly Heavy Metal-Resistant Bacterium from a Contaminated Stream in Amazonas, Brazil

2018 ◽  
Vol 6 (22) ◽  
Author(s):  
Maria Clara Tavares Astolfi ◽  
Elen Bethleen de Souza Carvalho ◽  
Adriane Menezes de Barros ◽  
Marcelo Valente Pinto ◽  
Luna Barrôco de Lacerda ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Draft Genome ◽  
Enterobacter Cloacae ◽  
The Novel ◽  
Biotechnological Applications ◽  
Toxic Compounds ◽  
Content Type ◽  
Genes Encoding ◽  
Heavy Metal Resistant

ABSTRACT Here, we report the draft genome of the Enterobacter cloacae strain amazonensis, a bacterium highly resistant to mercury that was isolated from a metal- and sewage-contaminated stream in Amazonas, Brazil. The exploration of the 5.0-Mb genome revealed 104 genes encoding resistance to toxic compounds and heavy metals, highlighting the potential biotechnological applications of this strain.

scholarly journals First Draft Genome Sequence of Xanthomonas axonopodis pv. eucalyptorum, Causal Agent of Bacterial Leaf Blight on Eucalypt

2019 ◽  
Vol 8 (16) ◽  
Author(s):  
Yane F. Neves ◽  
Samuel A. Santos ◽  
Lúcio M. S. Guimarães ◽  
Pedro M. P. Vidigal ◽  
Jorge L. Badel ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Bacterial Blight ◽  
Molecular Mechanisms ◽  
Draft Genome ◽  
Genomic Data ◽  
Leaf Blight ◽  
Bacterial Leaf Blight ◽  
Draft Genome Sequence ◽  
Content Type ◽  
Xanthomonas Axonopodis

Here, we report the annotated draft genome sequence of Xanthomonas axonopodis pv. eucalyptorum pathotype strain LPF602 (synonym Xanthomonas axonopodis BSC45a), isolated from eucalypt leaves showing bacterial blight symptoms in Brazil. The availability of these genomic data will help improve the understanding of the evolution and molecular mechanisms involved in the pathogenesis of this microorganism.

scholarly journals High-Quality Draft Genome Sequences of Three Cyanobacteria Isolated from the Limestone Walls of the Old Cathedral of Coimbra, Portugal

2020 ◽  
Vol 9 (26) ◽  
Author(s):  
Fabiana Soares ◽  
João Trovão ◽  
Catarina Coelho ◽  
Inês Costa ◽  
Nuno Mesquita ◽  
...  
Keyword(s):  
Draft Genome ◽  
World Heritage Site ◽  
The Novel ◽  
Genome Sequences ◽  
High Quality ◽  
Content Type ◽  
Heritage Site ◽  
Unesco World Heritage Site ◽  
Novel Taxa ◽  
High Quality Genome

ABSTRACT The recently described species Myxacorys almedinensis and two other cyanobacteria were isolated from the limestone walls of the Old Cathedral of Coimbra, Portugal (UNESCO World Heritage Site). The high-quality genome sequences presented here will be essential for characterization purposes and description of the novel taxa.

scholarly journals Draft Genome Sequence of KCTC 12630, the Type Strain of the Novel Species Sphingomonas ginsengisoli

2019 ◽  
Vol 8 (2) ◽  
Author(s):  
Yan-Qiong Li ◽  
Hui Zhang ◽  
Manik Prabhu Narsing Rao ◽  
Zhou-Yan Dong ◽  
Dalal Hussien M. Alkhalifah ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Type Strain ◽  
Novel Species ◽  
Draft Genome ◽  
Draft Genome Sequence ◽  
The Novel ◽  
Content Type

We report here the draft genome sequence of Sphingomonas ginsengisoli KCTC 12630T. The draft genome has a size of 3,045,889 bp and a G+C content of 67.1%.

scholarly journals Draft Genome Sequence of Rhizobium sp. Strain TBD182, an Antagonist of the Plant-Pathogenic Fungus Fusarium oxysporum, Isolated from a Novel Hydroponics System Using Organic Fertilizer

2017 ◽  
Vol 5 (11) ◽  
Author(s):  
Yuichiro Iida ◽  
Kazuki Fujiwara ◽  
Nobutaka Someya ◽  
Makoto Shinohara
Keyword(s):  
Fusarium Oxysporum ◽  
Genome Sequence ◽  
Molecular Mechanisms ◽  
Pathogenic Fungus ◽  
Organic Fertilizer ◽  
Draft Genome ◽  
Draft Genome Sequence ◽  
Content Type ◽  
Fungistatic Activity ◽  
Rhizobium Sp

ABSTRACT Rhizobium sp. strain TBD182, isolated from a novel hydroponics system, is an antagonistic bacterium that inhibits the mycelial growth of Fusarium oxysporum but does not eliminate the pathogen. We report the draft genome sequence of TBD182, which may contribute to elucidation of the molecular mechanisms of its fungistatic activity.

scholarly journals Leekyejoonella antrihumi gen. nov., sp. nov., a new member of the family Dermacoccaceae isolated from a cave soil

2020 ◽  
Vol 70 (5) ◽  
pp. 3340-3347 ◽  
Author(s):  
Soon Dong Lee ◽  
In Seop Kim ◽  
Peter Schumann ◽  
Gwanpil Song
Keyword(s):  
Type Species ◽  
Phylogenetic Analyses ◽  
Draft Genome ◽  
Rrna Gene ◽  
The Novel ◽  
Unidentified Phospholipid ◽  
Content Type ◽  
Link Type ◽  
The Family ◽  
Type Strains

A novel Gram-stain-positive, actinobacterial strain, designated C5-26T, was isolated from soil from a natural cave in Jeju, Republic of Korea, and its taxonomic position was investigated using a polyphasic approach. The organism was aerobic, and cells were non-spore-forming, non-motile cocci that occurred singly, in pairs, in triplets, in tetrads, in short chains or in irregular clusters. Colonies of the cells were circular, convex, entire and white. The peptidoglycan type was A4α with an l-Ser–d-Asp interpeptide bridge. The whole-cell sugars comprised glucose, rhamnose, mannose, arabinose, galactose and ribose. The major menaquinone was MK-8(H4). The polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and an unidentified phospholipid. The major fatty acids were iso-C16 : 0 and iso-C16 : 1 h. The size of the draft genome was 5.32 Mbp with depth of coverage of 161×. The G+C content of the genomic DNA was 67.1 mol%. Phylogenetic analyses based on 16S rRNA gene sequences showed that the novel isolate belonged to the family Dermacoccaceae and formed a distinct subcluster at the base of the radiation of the genus Luteipulveratus . Highest sequence similarities of the novel isolate were found to the type strains of Luteipulveratus halotolerans (96.2 %), Branchiibius hedensis (95.4 %), Luteipulveratus mongoliensis (95.4 %) and Branchiibius cervicis (95.3 %). The whole genome-based phylogeny supported the novelty of the isolate at the genus level in the family Dermacoccaceae . On the basis of data from this polyphasic study, strain C5-26T (=KCTC 39632T=DSM 108676T) represents a novel species of a new genus in the family Dermacoccaceae , for which the name Leekyejoonella antrihumi gen. nov., sp. nov. is proposed.

scholarly journals Draft Genome Sequences of Six Vibrio Strains Isolated from the Atlantic Intertidal Marine Sponge Ophlitaspongia papilla

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Anoop Alex ◽  
Vijayakumari Pratheepa ◽  
Joana Martins ◽  
Agostinho Antunes
Keyword(s):  
Comparative Genomics ◽  
Marine Sponge ◽  
Molecular Mechanisms ◽  
Draft Genome ◽  
Genome Mining ◽  
Genome Sequences ◽  
Content Type ◽  
Bioactive Potential ◽  
Microbial Symbioses

ABSTRACT We report here the genome sequences of six Vibrio strains isolated from an Atlantic intertidal marine sponge, Ophlitaspongia papilla. Genome mining and comparative genomics will assist in deciphering the bioactive potential of the symbiotic microbes and molecular mechanisms of sponge-microbial symbioses.

scholarly journals Draft Genome Sequence of Lactobacillus paraplantarum OSY-TC318, a Producer of the Novel Lantibiotic Paraplantaracin TC318

2019 ◽  
Vol 8 (19) ◽  
Author(s):  
Walaa E. Hussein ◽  
En Huang ◽  
Ismet Ozturk ◽  
Ahmed E. Yousef
Keyword(s):  
Genome Size ◽  
Genome Sequence ◽  
Draft Genome ◽  
Gc Content ◽  
Peptide Sequencing ◽  
Draft Genome Sequence ◽  
The Novel ◽  
Content Type ◽  
A Genome ◽  
Lactobacillus Paraplantarum

Lactobacillus paraplantarum OSY-TC318 was isolated from Turkish Tulum cheese and found to produce a potent anti-Gram-positive peptide. Sequencing of the OSY-TC318 genome revealed a genome size of 3,587,488 bp and an average GC content of 43.4%.

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