scholarly journals Comparison of Planktonic and Biofilm Cultures of Pseudomonas fluorescens DSM 8341 Cells Grown on Fluoroacetate

2009 ◽  
Vol 75 (9) ◽  
pp. 2899-2907 ◽  
Author(s):  
Barry Heffernan ◽  
Cormac D. Murphy ◽  
Eoin Casey
Keyword(s):  
Cell Cycle ◽  
Pseudomonas Fluorescens ◽  
Biofilm Reactor ◽  
Overflow Metabolism ◽  
Planktonic Cells ◽  
Cell Cycle Kinetics ◽  
Physiological Properties ◽  
Similar Growth ◽  
Spatial Stratification

ABSTRACT Comparisons between the physiological properties of Pseudomonas fluorescens biofilm cells grown in a tubular biofilm reactor and planktonic cells grown in a chemostat were performed. Fluoroacetate was the sole carbon source for all experiments. The performance of cells was assessed using cell cycle kinetics and by determining specific fluoroacetate utilization rates. Cell cycle kinetics were studied by flow cytometry in conjunction with the fluorescent stain propidium iodide. Determination of the DNA content of planktonic and biofilm cultures showed little difference between the two modes of growth. Cultures with comparable specific glycolate utilization rates had similar percentages of cells in the B phase of the cell cycle, indicating similar growth rates. Specific fluoroacetate utilization rates showed the performance of planktonic cells to be superior to that of biofilm cells, with more fluoroacetate utilized per cell at similar specific fluoroacetate loading rates. A consequence of this decreased biofilm performance was the accumulation of glycolate in the effluent of biofilm cultures. This accumulation of glycolate was not observed in the effluent of planktonic cultures. Spatial stratification of oxygen within the biofilm was identified as a possible explanation for the overflow metabolism of glycolate and the decreased performance of the biofilm cells.

scholarly journals Determination of lethal concentration and antibacterial activity of commonly used disinfectants

1970 ◽  
Vol 1 (4) ◽  
pp. 102-105 ◽  
Author(s):  
M Alam ◽  
MM Rahman ◽  
MJ Foysal ◽  
MN Hossain
Keyword(s):  
Methylene Blue ◽  
Pseudomonas Fluorescens ◽  
Potassium Permanganate ◽  
Malachite Green ◽  
Copper Sulfate ◽  
Pathogenic Bacteria ◽  
Labeo Rohita ◽  
Inhibitory Effect ◽  
Lethal Concentration

The toxic effects of four disinfectants viz., copper sulfate (CuSO4), potassium permanganate (KMnO4), methylene blue and malachite green on fish and fish pathogenic bacteria Aeromonas sp., Pseudomonas fluorescens, Edwardsiella sp. and Flavobacterium sp. were investigated. Lethal concentration of the disinfectants to fingerlings of Labeo rohita was determined in aquarium by standard method. Lethal concentration of copper sulfate (CuSO4), potassium permanganate (KMnO4), methylene blue and malachite green against fish were found in 0.75ppm, 7ppm, 6ppm and 0.5ppm at 21.4hrs, 18hrs, 9.5hrs and 1.40hrs, respectively. Methylene blue at 4ppm and 5ppm concentration inhibited the growth of Pseudomonas fluorescens and 6ppm concentration suppressed the growth of Aeromonas sp. Copper sulfate (CuSO4) was effective only against Edwardsiella sp at concentration of 10ppm and 8ppm. Malachite green repressed the growth of all four tasted bacteria at a concentration of 1ppm. Potassium permanganate (KMnO4) was failed to exhibit any inhibitory effect on the bacteria even at 30ppm concentration. DOI: http://dx.doi.org/10.3329/ijns.v1i4.9738 IJNS 2011 1(4): 102-105

Sources of variation in intermitotic time and their relation to cell cycle kinetics

1981 ◽  
Vol 93 (4) ◽  
pp. 1037-1045 ◽  
Author(s):  
E.J.J. van Zoelen ◽  
P.T. van der Saag ◽  
S.W. de Laat
Keyword(s):  
Cell Cycle ◽  
Cell Cycle Kinetics ◽  
Sources Of Variation

Label-Free Determination of the Cell Cycle Phase in Human Embryonic Stem Cells by Raman Microspectroscopy

2013 ◽  
Vol 85 (19) ◽  
pp. 8996-9002 ◽  
Author(s):  
Stanislav O. Konorov ◽  
H. Georg Schulze ◽  
James M. Piret ◽  
Michael W. Blades ◽  
Robin F. B. Turner
Keyword(s):  
Cell Cycle ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Raman Microspectroscopy ◽  
Cell Cycle Phase ◽  
Cycle Phase ◽  
Label Free

Demonstration of cell cycle kinetics in thyroid primary culture by immunostaining of proliferating cell nuclear antigen: differences in cyclic AMP-dependent and -independent mitogenic stimulations

1993 ◽  
Vol 105 (1) ◽  
pp. 69-80 ◽  
Author(s):  
M. Baptist ◽  
J.E. Dumont ◽  
P.P. Roger
Keyword(s):  
Cell Cycle ◽  
Dna Synthesis ◽  
Primary Culture ◽  
Proliferating Cell Nuclear Antigen ◽  
Cell Cycle Progression ◽  
Nuclear Antigen ◽  
Major Influence ◽  
Cell Cycle Kinetics ◽  
Experimental Conditions ◽  
Proliferating Cell

In this study, experimental conditions are described that allowed us to follow the fate of the DNA polymerase delta-associated proliferating cell nuclear antigen (PCNA), by immunolabeling during the overall cell cycle. Differences in subcellular localization or the presence of PCNA allowed us to identify each phase of the cell cycle. Using these cell cycle markers in dog thyroid epithelial cells in primary culture, we found unexpected differences in cell cycle kinetics, in response to stimulations through cAMP-dependent and cAMP-independent pathways. These provide a new dimension to the view that the two pathways are largely separate, but co-operate on DNA synthesis initiation. More precisely, thyrotropin (TSH), acting via cAMP, exerts a potent triggering effect on DNA synthesis, associated with a precocious induction of PCNA appearance. This constitutes the major influence of TSH (cAMP) in determining cell cycle progression, which is only partly moderated by TSH-dependent lengthening of S- and G2-phases.

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