scholarly journals Establishment of a Gene Expression System in Ochrobactrum anthropi

2006 ◽  
Vol 72 (10) ◽  
pp. 6833-6836 ◽  
Author(s):  
Mohamed N. Seleem ◽  
Mohammed Ali ◽  
Stephen M. Boyle ◽  
Biswarup Mukhopadhyay ◽  
Sharon G. Witonsky ◽  
...  
Keyword(s):  
Gene Expression ◽  
Genetic Manipulation ◽  
Expression System ◽  
Protein Detection ◽  
Ochrobactrum Anthropi ◽  
Genetic Studies ◽  
Gene Expression System ◽  
His Tag ◽  
N Terminus

ABSTRACT Genetic studies of Ochrobactrum anthropi are hindered by the lack of a suitable gene expression system. We constructed a set of vectors containing several promoters and a His tag fusion in the N terminus to facilitate protein detection and purification. The new vectors should significantly enhance the genetic manipulation and characterization of O. anthropi.

scholarly journals Harnessing a Transient Gene Expression System in Nicotiana benthamiana to Explore Plant Agrochemical Transporters

Plants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 524
Author(s):  
Bingqi Wu ◽  
Zhiting Chen ◽  
Xiaohui Xu ◽  
Ronghua Chen ◽  
Siwei Wang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transient Expression ◽  
Nicotiana Benthamiana ◽  
Heterologous Gene Expression ◽  
Expression System ◽  
Functional Characterization ◽  
Transient Gene Expression ◽  
High Mobility ◽  
Gene Expression System

Functional characterization of plant agrichemical transporters provided an opportunity to discover molecules that have a high mobility in plants and have the potential to increase the amount of pesticides reaching damage sites. Agrobacterium-mediated transient expression in tobacco is simple and fast, and its protein expression efficiency is high; this system is generally used to mediate heterologous gene expression. In this article, transient expression of tobacco nicotine uptake permease (NtNUP1) and rice polyamine uptake transporter 1 (OsPUT1) in Nicotiana benthamiana was performed to investigate whether this system is useful as a platform for studying the interactions between plant transporters and pesticides. The results showed that NtNUP1 increases nicotine uptake in N. benthamiana foliar discs and protoplasts, indicating that this transient gene expression system is feasible for studying gene function. Moreover, yeast expression of OsPUT1 apparently increases methomyl uptake. Overall, this method of constructing a transient gene expression system is useful for improving the efficiency of analyzing the functions of plant heterologous transporter-encoding genes and revealed that this system can be further used to study the functions of transporters and pesticides, especially their interactions.

scholarly journals Characterization of the ethanol-inducible alc gene expression system in tomato

2005 ◽  
Vol 56 (416) ◽  
pp. 1635-1642 ◽  
Author(s):  
G. Ali Garoosi ◽  
Michael G. Salter ◽  
Mark X. Caddick ◽  
A. Brian Tomsett
Keyword(s):  
Gene Expression ◽  
Expression System ◽  
Gene Expression System

scholarly journals Characterization of the ethanol-inducible alc gene-expression system in Arabidopsis thaliana

2001 ◽  
Vol 28 (2) ◽  
pp. 225-235 ◽  
Author(s):  
Hairul A. Roslan ◽  
Michael G. Salter ◽  
Chris D. Wood ◽  
Michael R. H. White ◽  
Kevan P. Croft ◽  
...  
Keyword(s):  
Gene Expression ◽  
Arabidopsis Thaliana ◽  
Expression System ◽  
Gene Expression System

Development and characterization of a binary gene expression system based on bacteriophage T7 components in adenovirus vectors

Gene ◽  
1997 ◽  
Vol 193 (2) ◽  
pp. 129-140 ◽  
Author(s):  
Rosella Tomanin ◽  
Andrew J Bett ◽  
Luigi Picci ◽  
Maurizio Scarpa ◽  
Frank L Graham
Keyword(s):  
Gene Expression ◽  
Expression System ◽  
Bacteriophage T7 ◽  
Gene Expression System ◽  
Adenovirus Vectors

scholarly journals Artificial complementary chromatic acclimation gene expression system in Escherichia coli

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Dwi Ariyanti ◽  
Kazunori Ikebukuro ◽  
Koji Sode
Keyword(s):  
Gene Expression ◽  
Escherichia Coli ◽  
Light Exposure ◽  
Expression System ◽  
Red Light ◽  
Green Light ◽  
Light Illumination ◽  
Multiple Gene ◽  
Gene Expression System ◽  
Chromatic Acclimation

Abstract Background The development of multiple gene expression systems, especially those based on the physical signals, such as multiple color light irradiations, is challenging. Complementary chromatic acclimation (CCA), a photoreversible process that facilitates the control of cellular expression using light of different wavelengths in cyanobacteria, is one example. In this study, an artificial CCA systems, inspired by type III CCA light-regulated gene expression, was designed by employing a single photosensor system, the CcaS/CcaR green light gene expression system derived from Synechocystis sp. PCC6803, combined with G-box (the regulator recognized by activated CcaR), the cognate cpcG2 promoter, and the constitutively transcribed promoter, the PtrcΔLacO promoter. Results One G-box was inserted upstream of the cpcG2 promoter and a reporter gene, the rfp gene (green light-induced gene expression), and the other G-box was inserted between the PtrcΔLacO promoter and a reporter gene, the bfp gene (red light-induced gene expression). The Escherichia coli transformants with plasmid-encoded genes were evaluated at the transcriptional and translational levels under red or green light illumination. Under green light illumination, the transcription and translation of the rfp gene were observed, whereas the expression of the bfp gene was repressed. Under red light illumination, the transcription and translation of the bfp gene were observed, whereas the expression of the rfp gene was repressed. During the red and green light exposure cycles at every 6 h, BFP expression increased under red light exposure while RFP expression was repressed, and RFP expression increased under green light exposure while BFP expression was repressed. Conclusion An artificial CCA system was developed to realize a multiple gene expression system, which was regulated by two colors, red and green lights, using a single photosensor system, the CcaS/CcaR system derived from Synechocystis sp. PCC6803, in E. coli. The artificial CCA system functioned repeatedly during red and green light exposure cycles. These results demonstrate the potential application of this CCA gene expression system for the production of multiple metabolites in a variety of microorganisms, such as cyanobacteria.

Sign in / Sign up

Export Citation Format

Share Document