scholarly journals Antifungal Activity of Bacillus amyloliquefaciens NJN-6 Volatile Compounds against Fusarium oxysporum f. sp. cubense

2012 ◽  
Vol 78 (16) ◽  
pp. 5942-5944 ◽  
Author(s):  
Jun Yuan ◽  
Waseem Raza ◽  
Qirong Shen ◽  
Qiwei Huang
Keyword(s):  
Antifungal Activity ◽  
Fusarium Oxysporum ◽  
Volatile Compounds ◽  
Spore Germination ◽  
Bacillus Amyloliquefaciens ◽  
Fungal Growth ◽  
Content Type

ABSTRACTBacillus amyloliquefaciensNJN-6 produces volatile compounds (VOCs) that inhibit the growth and spore germination ofFusarium oxysporumf. sp.cubense. Among the total of 36 volatile compounds detected, 11 compounds completely inhibited fungal growth. The antifungal activity of these compounds suggested that VOCs can play important roles over short and long distances in the suppression ofFusarium oxysporum.

Antifungal activity of ajoene, a constituent of garlic (Allium sativum)

1990 ◽  
Vol 68 (6) ◽  
pp. 1354-1356 ◽  
Author(s):  
U. P. Singh ◽  
V. N. Pandey ◽  
K. G. Wagner ◽  
K. P. Singh
Keyword(s):  
Antifungal Activity ◽  
Fusarium Oxysporum ◽  
Spore Germination ◽  
Allium Sativum ◽  
Alternaria Solani ◽  
Field Conditions ◽  
Fusarium Udum ◽  
Alternaria Tenuissima ◽  
Fusarium Semitectum ◽  
Alternaria Sp

Ajoene, a compound derived from garlic (Allium sativum L.), inhibited spore germination of some fungi, namely, Alternaria solani, Alternaria tenuissima, Alternaria triticina, Alternaria sp., Colletotrichum sp., Curvularia sp., Fusarium lini, Fusarium oxysporum, Fusarium semitectum, and Fusarium udum, which cause serious diseases in some important crop plants in India. The compound was very effective in checking spore germination at a concentration of 25 μg/mL in some of the above fungi and, in most cases, there was 100% inhibition of germination at 100 μg/mL. It is quite likely that the compound may be useful in controlling disease(s) under field conditions.

scholarly journals Characterization of Antifungal Activity and Nail Penetration of ME1111, a New Antifungal Agent for Topical Treatment of Onychomycosis

2015 ◽  
Vol 60 (2) ◽  
pp. 1035-1039 ◽  
Author(s):  
Yuji Tabata ◽  
Naomi Takei-Masuda ◽  
Natsuki Kubota ◽  
Sho Takahata ◽  
Makoto Ohyama ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Antifungal Agent ◽  
Fungal Growth ◽  
Trichophyton Mentagrophytes ◽  
Dependent Manner ◽  
Content Type ◽  
Etiologic Agents ◽  
Prevalent Disease ◽  
Penetration Ability

ABSTRACTFungal nail infection (onychomycosis) is a prevalent disease in many areas of the world, with a high incidence approaching 23%. Available antifungals to treat the disease suffer from a number of disadvantages, necessitating the discovery of new efficacious and safe antifungals. Here, we evaluate thein vitroantifungal activity and nail penetration ability of ME1111, a novel antifungal agent, along with comparator drugs, including ciclopirox, amorolfine, terbinafine, and itraconazole. ME1111 showed potent antifungal activity againstTrichophyton rubrumandTrichophyton mentagrophytes(the major etiologic agents of onychomycosis) strains isolated in Japan and reference fungal strains with an MIC range of 0.12 to 0.5 mg/liter and an MIC50and MIC90of 0.5 mg/liter for both. Importantly, none of the tested isolates showed an elevated ME1111 MIC. Moreover, the antifungal activity of ME1111 was minimally affected by 5% wool keratin powder in comparison to the other antifungals tested. The ME1111 solution was able to penetrate human nails and inhibit fungal growth in a dose-dependent manner according to the TurChub assay. In contrast, 8% ciclopirox and 5% amorolfine nail lacquers showed no activity under the same conditions. ME1111 demonstrated approximately 60-fold-greater selectivity in inhibition ofTrichophyton spp. than of human cell lines. Our findings demonstrate that ME1111 possesses potent antidermatophyte activity, maintains this activity in the presence of keratin, and possesses excellent human nail permeability. These results suggest that ME1111 is a promising topical medication for the treatment of onychomycosis and therefore warrants further clinical evaluation.

The killer yeast Wickerhamomyces anomalus Cf20 exerts a broad anti-Candida activity through the production of killer toxins and volatile compounds

2020 ◽  
Vol 58 (8) ◽  
pp. 1102-1113 ◽  
Author(s):  
Miguel Fernández de Ullivarri ◽  
Gabriela A Bulacios ◽  
Silvia A Navarro ◽  
Lucía Lanza ◽  
Lucia M Mendoza ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Volatile Compounds ◽  
Opportunistic Infections ◽  
Killer Toxin ◽  
Fungal Growth ◽  
Candida Spp ◽  
Killer Yeast ◽  
Wickerhamomyces Anomalus ◽  
Fungistatic Activity ◽  
Killer Toxins

Abstract Candidiasis is a group of opportunistic infections caused by yeast of the genus Candida. The appearance of drug resistance and the adverse effects of current antifungal therapies require the search for new, more efficient therapeutic alternatives. Killer yeasts have aroused as suitable candidates for mining new antifungal compounds. Killer strains secrete antimicrobial proteins named killer toxins, with promissory antifungal activity. Here we found that the killer yeast Wickerhamomyces anomalus Cf20 and its cell-free supernatant (CFS) inhibited six pathogenic strains and one collection strain of Candida spp. The inhibition is mainly mediated by secreted killer toxins and, to a lesser extent, by volatile compounds such as acetic acid and ethyl acetate. A new large killer toxin (>180 kDa) was purified, which exerted 70–74% of the total CFS anti-Candida activity, and the previously described glucanase KTCf20 was inhibitory in a lesser extent as well. In addition, we demonstrated that Cf20 possesses the genes encoding for the β-1,3-glucanases WaExg1 and WaExg2, proteins with extensively studied antifungal activity, particularly WaExg2. Finally, the 10-fold concentrated CFS exerted a high candidacidal effect at 37°C, completely inhibiting the fungal growth, although the nonconcentrated CFS (RCF 1) had very limited fungistatic activity at this temperature. In conclusion, W. anomalus Cf20 produces different low and high molecular weight compounds with anti-Candida activity that could be used to design new therapies for candidiasis and as a source for novel antimicrobial compounds as well.

scholarly journals Potential of Octanol and Octanal from Heracleum sosnowskyi Fruits for the Control of Fusarium oxysporum f. sp. lycopersici

2020 ◽  
Vol 12 (22) ◽  
pp. 9334
Author(s):  
May Khaing Hpoo ◽  
Maryia Mishyna ◽  
Valery Prokhorov ◽  
Tsutomu Arie ◽  
Akihito Takano ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Fusarium Oxysporum ◽  
Volatile Compounds ◽  
Mycelial Growth ◽  
Inhibitory Effect ◽  
Heracleum Sosnowskyi ◽  
Half Maximal Effective Concentration ◽  
Inhibitory Compound ◽  
Race 1 ◽  
Race 2

The antifungal activity of volatile compounds from the fruit, leaf, rhizome and root of 109 plant species was evaluated against Fusarium oxysporum f. sp. lycopersici (FOL) race 1—the tomato wilt pathogen—by using the modified dish pack method. Eighty-eight plant samples inhibited mycelial growth, including volatiles from fruits of Heracleum sosnowskyi, which exhibited the strongest antifungal activity, showing 67% inhibition. Two volatile compounds from the fruits of H. sosnowskyi (octanol and octanal) and trans-2-hexenal as a control were tested for their antifungal activities against FOL race 1 and race 2. In terms of half-maximal effective concentration (EC50) values, octanol was found to be the most inhibitory compound for both pathogenic races, with the smallest EC50 values of 8.1 and 9.3 ng/mL for race 1 and race 2, respectively. In the biofumigation experiment, the lowest disease severity of tomato plants and smallest conidial population of race 1 and race 2 were found in trans-2-hexenal and octanol treated soil, while octanal had an inhibitory effect only on race 2. Therefore, our study demonstrated the effectiveness of volatile octanol and trans-2-hexenal on the control of the mycelial growth of two races of Fusarium oxysporum f. sp. lycopersici and may have potential for the future development of novel biofumigants.

scholarly journals The Effects of Heat Activation on Bacillus Spore Germination, with Nutrients or under High Pressure, with or without Various Germination Proteins

2015 ◽  
Vol 81 (8) ◽  
pp. 2927-2938 ◽  
Author(s):  
Stephanie Luu ◽  
Jose Cruz-Mora ◽  
Barbara Setlow ◽  
Florence E. Feeherry ◽  
Christopher J. Doona ◽  
...  
Keyword(s):  
High Pressure ◽  
Spore Germination ◽  
Protein Conformation ◽  
Bacillus Amyloliquefaciens ◽  
Clostridium Botulinum ◽  
Nutrient Concentrations ◽  
Content Type ◽  
Bacillus Spore ◽  
Heat Activation ◽  
Activation Times

ABSTRACTNutrient germination of spores ofBacillusspecies occurs through germinant receptors (GRs) in spores' inner membrane (IM) in a process stimulated by sublethal heat activation.Bacillus subtilisspores maximum germination rates via different GRs required different 75°C heat activation times: 15 min forl-valine germination via the GerA GR and 4 h for germination with thel-asparagine–glucose–fructose–K+mixture via the GerB and GerK GRs, with GerK requiring the most heat activation. In some cases, optimal heat activation decreased nutrient concentrations for half-maximal germination rates. Germination of spores via various GRs by high pressure (HP) of 150 MPa exhibited heat activation requirements similar to those of nutrient germination, and the loss of the GerD protein, required for optimal GR function, did not eliminate heat activation requirements for maximal germination rates. These results are consistent with heat activation acting primarily on GRs. However, (i) heat activation had no effects on GR or GerD protein conformation, as probed by biotinylation by an external reagent; (ii) spores prepared at low and high temperatures that affect spores' IM properties exhibited large differences in heat activation requirements for nutrient germination; and (iii) spore germination by 550 MPa of HP was also affected by heat activation, but the effects were relatively GR independent. The last results are consistent with heat activation affecting spores' IM and only indirectly affecting GRs. The 150- and 550-MPa HP germinations ofBacillus amyloliquefaciensspores, a potential surrogate forClostridium botulinumspores in HP treatments of foods, were also stimulated by heat activation.

scholarly journals Rotenone Enhances the Antifungal Properties of Staurosporine

2010 ◽  
Vol 9 (6) ◽  
pp. 906-914 ◽  
Author(s):  
Ana Castro ◽  
Catarina Lemos ◽  
Artur Falcão ◽  
Andreia S. Fernandes ◽  
N. Louise Glass ◽  
...  
Keyword(s):  
Cell Death ◽  
Antifungal Activity ◽  
Complex I ◽  
Specific Inhibitor ◽  
Fungal Growth ◽  
Chain Complex ◽  
Glutathione Depletion ◽  
Oxygen Species ◽  
Content Type ◽  
The Common

ABSTRACT We studied staurosporine-induced cell death in the filamentous fungus Neurospora crassa. The generation of reactive oxygen species during the process appears to be an important signaling event, since addition of the antioxidant glutathione prevents the effects of staurosporine on fungal growth. Selected mutants with mutations in respiratory chain complex I are extremely sensitive to the drug, stressing the involvement of complex I in programmed cell death. Following this finding, we determined that the complex I-specific inhibitor rotenone used in combination with staurosporine results in a synergistic and specific antifungal activity, likely through a concerted action on intracellular glutathione depletion. Paradoxically, the synergistic antifungal activity of rotenone and staurosporine is observed in N. crassa complex I mutants and in Saccharomyces cerevisiae, which lacks complex I. In addition, it is not observed when other complex I inhibitors are used instead of rotenone. These results indicate that the rotenone effect is independent of complex I inhibition. The combination of rotenone and staurosporine is effective against N. crassa as well as against the common pathogens Aspergillus fumigatus and Candida albicans, pointing to its usefulness as an antifungal agent.

scholarly journals Antifungal Hydroxy Fatty Acids Produced during Sourdough Fermentation: Microbial and Enzymatic Pathways, and Antifungal Activity in Bread

2013 ◽  
Vol 79 (6) ◽  
pp. 1866-1873 ◽  
Author(s):  
Brenna A. Black ◽  
Emanuele Zannini ◽  
Jonathan M. Curtis ◽  
Michael G. Gänzle
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Linoleic Acid ◽  
Antifungal Activity ◽  
Octadecenoic Acid ◽  
Fungal Growth ◽  
Hydroxy Fatty Acids ◽  
Enzymatic Oxidation ◽  
Bread Making ◽  
Content Type

ABSTRACTLactobacilli convert linoleic acid to hydroxy fatty acids; however, this conversion has not been demonstrated in food fermentations and it remains unknown whether hydroxy fatty acids produced by lactobacilli have antifungal activity. This study aimed to determine whether lactobacilli convert linoleic acid to metabolites with antifungal activity and to assess whether this conversion can be employed to delay fungal growth on bread. Aqueous and organic extracts from seven strains of lactobacilli grown in modified De Man Rogosa Sharpe medium or sourdough were assayed for antifungal activity.Lactobacillus hammesiiexhibited increased antifungal activity upon the addition of linoleic acid as a substrate. Bioassay-guided fractionation attributed the antifungal activity ofL. hammesiito a monohydroxy C18:1fatty acid. Comparison of its antifungal activity to those of other hydroxy fatty acids revealed that the monohydroxy fraction fromL. hammesiiand coriolic (13-hydroxy-9,11-octadecadienoic) acid were the most active, with MICs of 0.1 to 0.7 g liter−1. Ricinoleic (12-hydroxy-9-octadecenoic) acid was active at a MIC of 2.4 g liter−1.L. hammesiiaccumulated the monohydroxy C18:1fatty acid in sourdough to a concentration of 0.73 ± 0.03 g liter−1(mean ± standard deviation). Generation of hydroxy fatty acids in sourdough also occurred through enzymatic oxidation of linoleic acid to coriolic acid. The use of 20% sourdough fermented withL. hammesiior the use of 0.15% coriolic acid in bread making increased the mold-free shelf life by 2 to 3 days or from 2 to more than 6 days, respectively. In conclusion,L. hammesiiconverts linoleic acid in sourdough and the resulting monohydroxy octadecenoic acid exerts antifungal activity in bread.

scholarly journals Antifungal activity of Bacillus amyloliquefaciens against Fusarium oxysporum f. sp. cubense race 1

2021 ◽  
pp. 466-478
Author(s):  
Mileidy Cruz Martín ◽  
Liliana Leyva ◽  
Mayra Acosta Suárez ◽  
Tatiana Pichardo ◽  
Idalmis Bermúdez Caraballoso ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Fusarium Oxysporum ◽  
Fusarium Wilt ◽  
Culture Filtrate ◽  
Bacillus Amyloliquefaciens ◽  
Biological Control Agent ◽  
Antifungal Effect ◽  
Antagonistic Microorganisms ◽  
Race 1

Introduction. Due to the absence of totally effective either economically viable chemical agents for the control of Fusarium wilt, the use of antagonistic microorganisms is of great interest since it could represent a more economically and ecologically sustainable alternative. Objective. To analyze the antifungal effect of the Bacillus amyloliquefaciens CCIBP-A5 strain against Fusarium oxysporum. Materials and methods. The work was carried out in the Laboratory of Applied Microbiology of the Instituto de Biotecnología de las Plantas, Cuba, between September 2017 and June, 2018. The in vitro and in vivo antifungal activity of its culture filtrate and cell against F. oxysporum has been assayed. Results. The results indicated that the metabolites present in the culture filtrate of B. amyloliquefaciens CCIBP-A5 significantly influenced the growth and morphology of the mycelium and the conidia. They also caused oxidative damage to the lipid molecules of F. oxysporum. In addition, this strain showed inhibitory effects on the development of the disease under controlled conditions. These aspects are key when selecting a bacterial candidate as a biological control agent. Conclusions. The results showed that the B. amyloliquefaciens CCIBP-A5 strain, isolated from Musa sp., had an in vitro antifungal effect against the vegetative and reproductive structures of Foc race 1 as well as on the Musa spp.-F. oxysporum interaction. This strain is suggested for the development of a bioproduct for Fusarium wilt management.

scholarly journals A Thermotolerant Marine Bacillus amyloliquefaciens S185 Producing Iturin A5 for Antifungal Activity against Fusarium oxysporum f. sp. cubense

Marine Drugs ◽  
2021 ◽  
Vol 19 (9) ◽  
pp. 516
Author(s):  
Pratiksha Singh ◽  
Jin Xie ◽  
Yanhua Qi ◽  
Qijian Qin ◽  
Cheng Jin ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Fusarium Oxysporum ◽  
Bacillus Amyloliquefaciens ◽  
Morphological Changes ◽  
Carbon Sources ◽  
Cost Effective ◽  
Antifungal Compound ◽  
Ionization Mass ◽  
Panama Disease ◽  
Plant Growth Promoting

Fusarium wilt of banana (also known as Panama disease), is a severe fungal disease caused by soil-borne Fusarium oxysporum f. sp. cubense (Foc). In recent years, biocontrol strategies using antifungal microorganisms from various niches and their related bioactive compounds have been used to prevent and control Panama disease. Here, a thermotolerant marine strain S185 was identified as Bacillus amyloliquefaciens, displaying strong antifungal activity against Foc. The strain S185 possesses multiple plant growth-promoting (PGP) and biocontrol utility properties, such as producing indole acetic acid (IAA) and ammonia, assimilating various carbon sources, tolerating pH of 4 to 9, temperature of 20 to 50 °C, and salt stress of 1 to 5%. Inoculation of S185 colonized the banana plants effectively and was mainly located in leaf and root tissues. To further investigate the antifungal components, compounds were extracted, fractionated, and purified. One compound, inhibiting Foc with minimum inhibitory concentrations (MICs) of 25 μg/disk, was identified as iturin A5 by high-resolution electrospray ionization mass spectrometry (HR-ESI-MS) and nuclear magnetic resonance (NMR). The isolated iturin, A5, resulted in severe morphological changes during spore germination and hyphae growth of Foc. These results specify that B. amyloliquefaciens S185 plays a key role in preventing the Foc pathogen by producing the antifungal compound iturin A5, and possesses potential as a cost-effective and sustainable biocontrol strain for Panama disease in the future. This is the first report of isolation of the antifungal compound iturin A5 from thermotolerant marine B. amyloliquefaciens S185.

Sign in / Sign up

Export Citation Format

Share Document