scholarly journals Identification of Bacillus cereus Group Species Associated with Food Poisoning Outbreaks in British Columbia, Canada

2008 ◽  
Vol 74 (23) ◽  
pp. 7451-7453 ◽  
Author(s):  
Lorraine McIntyre ◽  
Kathryn Bernard ◽  
Daniel Beniac ◽  
Judith L. Isaac-Renton ◽  
David Craig Naseby
Keyword(s):  
British Columbia ◽  
Bacillus Cereus ◽  
Food Poisoning ◽  
Bacillus Cereus Group ◽  
Group Species

ABSTRACT Food poisoning laboratories identify Bacillus cereus using routine methods that may not differentiate all Bacillus cereus group species. We recharacterized Bacillus food-poisoning strains from 39 outbreaks and identified B. cereus in 23 outbreaks, B. thuringiensis in 4, B. mycoides in 1, and mixed strains of Bacillus in 11 outbreaks.

scholarly journals The Genetically Remote Pathogenic Strain NVH391-98 of the Bacillus cereus Group Is Representative of a Cluster of Thermophilic Strains

2007 ◽  
Vol 74 (4) ◽  
pp. 1276-1280 ◽  
Author(s):  
Sandrine Auger ◽  
Nathalie Galleron ◽  
Elena Bidnenko ◽  
S. Dusko Ehrlich ◽  
Alla Lapidus ◽  
...  
Keyword(s):  
Bacillus Cereus ◽  
Food Poisoning ◽  
Temperate Phage ◽  
Pathogenic Strain ◽  
Bacillus Cereus Group ◽  
Close Relatives

ABSTRACT Bacteria of the Bacillus cereus group are known to cause food poisoning. A rare phylogenetically remote strain, NVH391-98, was recently characterized to encode a particularly efficient cytotoxin K presumably responsible for food poisoning. This pathogenic strain and its close relatives can be phenotypically distinguished from other strains of the B. cereus group by the inability to grow at temperatures below 17°C and by the ability to grow at temperatures from 48 to 53°C. A temperate phage, phBC391A2, residing in the genome of NVH391-98 allows us to distinguish the three known members of this thermophilic strain cluster.

scholarly journals Detection and quantification of viable Bacillus cereus group species in milk by propidium monoazide quantitative real-time PCR

2016 ◽  
Vol 99 (4) ◽  
pp. 2617-2624 ◽  
Author(s):  
Fernanda Cattani ◽  
Valdir C. Barth ◽  
Jéssica S.R. Nasário ◽  
Carlos A.S. Ferreira ◽  
Sílvia D. Oliveira
Keyword(s):  
Bacillus Cereus ◽  
Real Time ◽  
Real Time Pcr ◽  
Propidium Monoazide ◽  
Quantitative Real Time Pcr ◽  
Bacillus Cereus Group ◽  
Group Species ◽  
Detection And Quantification

scholarly journals Multi-locus sequence typing and virulence profile in Bacillus cereus sensu lato strains isolated from dairy products

2021 ◽  
Vol 9 (4) ◽  
Author(s):  
Angelica Bianco ◽  
Loredana Capozzi ◽  
Angela Miccolupo ◽  
Simona Iannetti ◽  
Maria Luisa Danzetta ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Bacillus Cereus ◽  
Dairy Products ◽  
Virulence Genes ◽  
Food Poisoning ◽  
Important Food ◽  
Food Contaminants ◽  
Bacillus Cereus Group ◽  
Mlst Analysis ◽  
Sequence Types

Members of Bacillus cereus group are important food contaminants and they are of relevant interest in food safety and public heath due to their ability to cause two distinct forms of food poisoning, emetic and diarrhoeal syndrome. In the present study, 90 strains of B. cereus isolated from dairy products, have been typed using Multilocus Sequence Typing (MLST) analysis and investigated for the occurrence of 10 enterotoxigenic genes (hblA, hblC, hblD, nheA, nheB, nheC, cytK, entFM, entS and bceT) and one emetogenic gene (ces), to determine their genetic diversity. A total of 58 sequence types were identified and among these 17 were signalled as new profiles. Among the virulence genes, the majority of our strains carried the entS (92%), entFM (86%), nhe (82%) and cytK (72%) genes. All remaining genes were identified in at least one strain with different prevalence, stressing the genetic diversity, how even the different grade of pathogenicity of B. cereus isolated from dairy products.

Design of specific DNA primers to detect the Bacillus cereus group species

2009 ◽  
Author(s):  
Catherine Adley ◽  
Khalil Arshak ◽  
Camila Molnar ◽  
Kamila Oliwa ◽  
Vijayalakshmi Velusamy
Keyword(s):  
Bacillus Cereus ◽  
Bacillus Cereus Group ◽  
Dna Primers ◽  
Group Species

scholarly journals A Novel Multiplex PCR Discriminates Bacillus anthracis and Its Genetically Related Strains from Other Bacillus cereus Group Species

PLoS ONE ◽  
2015 ◽  
Vol 10 (3) ◽  
pp. e0122004 ◽  
Author(s):  
Hirohito Ogawa ◽  
Daisuke Fujikura ◽  
Miyuki Ohnuma ◽  
Naomi Ohnishi ◽  
Bernard M. Hang'ombe ◽  
...  
Keyword(s):  
Bacillus Cereus ◽  
Bacillus Anthracis ◽  
Multiplex Pcr ◽  
Bacillus Cereus Group ◽  
Group Species

scholarly journals Complete Genome Sequence of Bacillus cereus Group Phage TsarBomba

2015 ◽  
Vol 3 (5) ◽  
Author(s):  
Ivan Erill ◽  
Steven M. Caruso
Keyword(s):  
Bacillus Cereus ◽  
Host Range ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Bacillus Cereus Group ◽  
Double Stranded Dna ◽  
Wide Host Range ◽  
Group Species

The Bacillus cereus group bacteriophage TsarBomba, a double-stranded DNA Myoviridae , was isolated from soil collected in Saratov, Russia. TsarBomba was found to be similar to Bacillus phages BCP78 and BCU4, and to have a wide host range among Bacillus cereus group species.

Conducting polymer based DNA biosensor for the detection of the Bacillus cereus group species

2009 ◽  
Author(s):  
Vijayalakshmi Velusamy ◽  
Khalil Arshak ◽  
Olga Korostynska ◽  
Kamila Oliwa ◽  
Catherine Adley
Keyword(s):  
Bacillus Cereus ◽  
Conducting Polymer ◽  
Dna Biosensor ◽  
Bacillus Cereus Group ◽  
Group Species

scholarly journals Discrimination of Psychrotrophic and Mesophilic Strains of the Bacillus cereus Group by PCR Targeting of Major Cold Shock Protein Genes

1998 ◽  
Vol 64 (9) ◽  
pp. 3525-3529 ◽  
Author(s):  
Kevin P. Francis ◽  
Ralf Mayr ◽  
Felix von Stetten ◽  
Gordon S. A. B. Stewart ◽  
Siegfried Scherer
Keyword(s):  
Bacillus Cereus ◽  
Dairy Products ◽  
Cold Shock ◽  
Food Poisoning ◽  
Cold Shock Protein ◽  
Pcr Assay ◽  
Specific Primers ◽  
Bacillus Cereus Group ◽  
Cold Adapted ◽  
Pcr Targeting

ABSTRACT Detection of psychrotrophic strains (those able to grow at or below 7°C) of the Bacillus cereus group (Bacillus cereus, Bacillus thuringiensis, and Bacillus mycoides) in food products is at present extremely slow with conventional microbiology. This is due to an inability to discriminate these cold-adapted strains from their mesophilic counterparts (those able to grow only above 7°C) by means other than growth at low temperature, which takes 5 to 10 days for detection. Here we report the development of a single PCR assay that, using major cold shock protein-specific primers and appropriate annealing temperatures, is capable of both rapidly identifying bacteria of the B. cereus group and discriminating between psychrotrophic and mesophilic strains. It is intended that this development help to more accurately predict the shelf life of refrigerated pasteurized food and dairy products and to reduce the incidence of food poisoning by psychrotrophic strains of the B. cereus group.

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