scholarly journals Molecular Indications of Protein Damage in Adenoviruses after UV Disinfection

2010 ◽  
Vol 77 (3) ◽  
pp. 1145-1147 ◽  
Author(s):  
Anne C. Eischeid ◽  
Karl G. Linden
Keyword(s):  
Cell Culture ◽  
Viral Proteins ◽  
Low Pressure ◽  
Protein Damage ◽  
Uv Disinfection ◽  
Medium Pressure ◽  
The Difference ◽  
Uv Lamp ◽  
Detailed Work

ABSTRACTAdenoviruses are resistant to monochromatic, low-pressure (LP) UV disinfection—but have been shown to be susceptible to inactivation by polychromatic, medium-pressure (MP) UV—when assayed using cell culture infectivity. One possible explanation for the difference between UV lamp types is that the additional UV wavelengths emitted by MP UV enable it to cause greater damage to viral proteins than LP UV. The objective of this study was to examine protein damage in adenoviruses treated with LP and MP UV. Results show that MP UV is more effective at damaging viral proteins at high UV doses, though LP UV caused some damage as well. To our knowledge, this study is the first to investigate protein damage in UV-treated adenovirus, and the overview presented here is expected to provide a basis for further, more detailed work.

scholarly journals Effects of UV Radiation on Photolyase and Implications with Regards to Photoreactivation following Low- and Medium-Pressure UV Disinfection

2007 ◽  
Vol 74 (1) ◽  
pp. 327-328 ◽  
Author(s):  
Jiangyong Hu ◽  
Puay Hoon Quek
Keyword(s):  
Uv Radiation ◽  
Uv Irradiation ◽  
Flavin Adenine Dinucleotide ◽  
Low Pressure ◽  
Uv Disinfection ◽  
Medium Pressure ◽  
Uv Lamps

ABSTRACT Photolyase activity following exposure to low-pressure (LP) and medium-pressure (MP) UV lamps was evaluated. MP UV irradiation resulted in a greater reduction in photolyase activity than LP UV radiation. The results suggest that oxidation of the flavin adenine dinucleotide in photolyase may have caused the decrease in activity.

Re-use of wastewater: preventing the recovery of pathogens by using medium-pressure UV lamp technology

2004 ◽  
Vol 50 (6) ◽  
pp. 337-344 ◽  
Author(s):  
B.F. Kalisvaart
Keyword(s):  
Wastewater Treatment Plants ◽  
Uv Light ◽  
Low Pressure ◽  
Energy Output ◽  
Uv Spectrum ◽  
Medium Pressure ◽  
Uv Lamp ◽  
Cellular Dna ◽  
Uv Technology ◽  
Term Performance

Ultraviolet (UV) light has become widely accepted as an alternative to chlorination or ozonation for wastewater disinfection. There are now over 2,000 wastewater treatment plants worldwide using either low- or medium-pressure UV technology. Recent studies investigating UV lamp technology, configuration, cleaning requirements and ageing, as well as long-term performance tests, have demonstrated beyond any doubt the effectiveness of UV in inactivating pathogens in wastewater. Research has also shown that, to ensure permanent inactivation and prevent the recovery of microorganisms following exposure to UV, a broad, “polychromatic” spectrum of UV wavelengths is necessary. These wavelengths inflict irreparable damage not only on cellular DNA, but on other molecules, such as enzymes, as well. Only medium-pressure UV lamps produce the necessary broad range of wavelengths; low-pressure lamps emit a single wavelength peak which only affects DNA. Polychromatic medium-pressure UV light is so effective because of the lampÕs exceptionally high UV energy output at specific wavelengths across the UV spectrum. It has been shown, for example, that pathogenic E. coli O175:H7 was able to repair the damage caused by low-pressure UV, but no repair was detected following exposure to UV from medium-pressure lamps.

Characterization of a commercially-available, low-pressure UV lamp as a disinfection system for decontamination of common nosocomial pathogens on N95 filtering facepiece respirator (FFR) material

2020 ◽  
Vol 6 (8) ◽  
pp. 2089-2102 ◽  
Author(s):  
C. Carolina Ontiveros ◽  
Crystal L. Sweeney ◽  
Chris Smith ◽  
Sean MacIsaac ◽  
Sebastian Munoz ◽  
...  
Keyword(s):  
Low Pressure ◽  
Uv Disinfection ◽  
Nosocomial Pathogens ◽  
Hospital Room ◽  
Uv Lamp

A commercially-available UV disinfection system used for hospital room disinfection was characterized and used for N95 filtering facepiece respirator (FFR) material disinfection.

scholarly journals Potential Repair of Escherichia coli DNA following Exposure to UV Radiation from Both Medium- and Low-Pressure UV Sources Used in Drinking Water Treatment

2002 ◽  
Vol 68 (7) ◽  
pp. 3293-3299 ◽  
Author(s):  
J. L. Zimmer ◽  
R. M. Slawson
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Water Treatment ◽  
Uv Radiation ◽  
Drinking Water Treatment ◽  
Low Pressure ◽  
Plate Count ◽  
Treatment Technology ◽  
Medium Pressure ◽  
Uv Lamp

ABSTRACT The increased use of UV radiation as a drinking water treatment technology has instigated studies of the repair potential of microorganisms following treatment. This study challenged the repair potential of an optimally grown nonpathogenic laboratory strain of Escherichia coli after UV radiation from low- and medium-pressure lamps. Samples were irradiated with doses of 5, 8, and 10 mJ/cm2 from a low-pressure lamp and 3, 5, 8, and 10 mJ/cm2 from a medium-pressure UV lamp housed in a bench-scale collimated beam apparatus. Following irradiation, samples were incubated at 37°C under photoreactivating light or in the dark. Sample aliquots were analyzed for up to 4 h following incubation using a standard plate count. Results of this study showed that E. coli underwent photorepair following exposure to the low-pressure UV source, but no repair was detectable following exposure to the medium-pressure UV source at the initial doses examined. Minimal repair was eventually observed upon medium-pressure UV lamp exposure when doses were lowered to 3 mJ/cm2. This study clearly indicates differences in repair potential under laboratory conditions between irradiation from low-pressure and medium-pressure UV sources of the type used in water treatment.

Inactivation differences of microorganisms by low pressure UV and pulsed xenon lamps

2003 ◽  
Vol 47 (3) ◽  
pp. 185-190 ◽  
Author(s):  
M. Otaki ◽  
A. Okuda ◽  
K. Tajima ◽  
T. Iwasaki ◽  
S. Kinoshita ◽  
...  
Keyword(s):  
Low Pressure ◽  
High Energy ◽  
Suppressive Effect ◽  
Uv Disinfection ◽  
Xenon Lamp ◽  
Survival Ratio ◽  
E Coli ◽  
High Turbidity ◽  
The Difference ◽  
Uv Lamps

UV disinfection has been applied to water treatment in recent years with low-pressure and medium-pressure UV lamps mainly used as the light source. In general, UV disinfection is considered to be inefficient with water of high turbidity because of inhibition of light penetration. Additionally, photoreactivation may be a problem that should be considered in case a disinfected water is discharged to the environment where sunlight causes reactivation. Recently, other types of lamps have been proposed including a flush-type lamp (such as a pulsed-xenon lamp) that emits high energy and wide wavelength intermittently. In this study, the difference between inactivation efficiencies by low-pressure UV (LPUV) and pulsed-xenon (PXe) lamps was investigated using two coliphage types and three strains of Escherichia coli. PXe had a suppressive effect on photoreactivation rate of the E. coli strains even though there was no significant effect on inactivation rate and maximum survival ratio after photoreactivation. PXe also had a benefit when applied to high turbidity waters as no tailing phenomena were observed in the low survival ratio area although it was observed in LPUV inactivation. This efficiency difference was considered to be due to the difference in irradiated wavelength of both lamps.

scholarly journals Photoreactivation of Escherichia coli after Low- or Medium-Pressure UV Disinfection Determined by an Endonuclease Sensitive Site Assay

2002 ◽  
Vol 68 (12) ◽  
pp. 6029-6035 ◽  
Author(s):  
Kumiko Oguma ◽  
Hiroyuki Katayama ◽  
Shinichiro Ohgaki
Keyword(s):  
Escherichia Coli ◽  
Uv Irradiation ◽  
Genomic Dna ◽  
Uv Disinfection ◽  
E Coli ◽  
Medium Pressure ◽  
Pyrimidine Dimers ◽  
Sensitive Site ◽  
Uv Lamp

ABSTRACT Photoreactivation of Escherichia coli after inactivation by a low-pressure (LP) UV lamp (254 nm), by a medium-pressure (MP) UV lamp (220 to 580 nm), or by a filtered medium-pressure (MPF) UV lamp (300 to 580 nm) was investigated. An endonuclease sensitive site (ESS) assay was used to determine the number of UV-induced pyrimidine dimers in the genomic DNA of E. coli, while a conventional cultivation assay was used to investigate the colony-forming ability (CFA) of E. coli. In photoreactivation experiments, more than 80% of the pyrimidine dimers induced by LP or MPF UV irradiation were repaired, while almost no repair of dimers was observed after MP UV exposure. The CFA ratios of E. coli recovered so that they were equivalent to 0.9-, 2.3-, and 1.7-log inactivation after 3-log inactivation by LP, MP, and MPF UV irradiation, respectively. Photorepair treatment of DNA in vitro suggested that among the MP UV emissions, wavelengths of 220 to 300 nm reduced the subsequent photorepair of ESS, possibly by causing a disorder in endogenous photolyase, an enzyme specific for photoreactivation. On the other hand, the MP UV irradiation at wavelengths between 300 and 580 nm was observed to play an important role in reducing the subsequent recovery of CFA by inducing damage other than damage to pyrimidine dimers. Therefore, it was found that inactivating light at a broad range of wavelengths effectively reduced subsequent photoreactivation, which could be an advantage that MP UV irradiation has over conventional LP UV irradiation.

Analysis and calculation of the process of low-pressure reverse osmosis during recycling of hygienic water

2019 ◽  
pp. 28-36
Author(s):  
Leonid S. Bobe ◽  
Nikolay A. Salnikov
Keyword(s):  
Mass Transfer ◽  
Reverse Osmosis ◽  
Life Support ◽  
Space Station ◽  
Low Pressure ◽  
Operating Pressure ◽  
Life Support System ◽  
Cleaning Agent ◽  
The Difference ◽  
Pressure Channel

Analysis and calculation have been conducted of the process of low-pressure reverse osmosis in the membrane apparatus of the system for recycling hygiene water for the space station. The paper describes the physics of the reverse osmosis treatment and determines the motive force of the process, which is the difference of effective pressures (operating pressure minus osmotic pressure) in the solution near the surface of the membrane and in the purified water. It is demonstrated that the membrane scrubbing action is accompanied by diffusion outflow of the cleaning agent components away from the membrane. The mass transfer coefficient and the difference of concentrations (and, accordingly, the difference of osmotic pressures) in the boundary layer of the pressure channel can be determined using an extended analogy between mass transfer and heat transfer. A procedure has been proposed and proven in an experiment for calculating the throughput of a reverse osmosis apparatus purifying the hygiene water obtained through the use of a cleaning agent used in sanitation and housekeeping procedures on Earth. Key words: life support system, hygiene water, water processing, low-pressure reverse osmosis, space station.

scholarly journals A Subglacial Boundary-layer Regelation Mechanism

1990 ◽  
Vol 36 (124) ◽  
pp. 263-268 ◽  
Author(s):  
E.M. Shoemaker
Keyword(s):  
Boundary Layer ◽  
Heat Input ◽  
High Water ◽  
Low Pressure ◽  
Critical Value ◽  
High Water Content ◽  
Overburden Pressure ◽  
Basal Ice ◽  
The Difference ◽  
Linear Nature

AbstractHeat input to basal ice at subglacial low-pressure regions, such as exist on the lee side of bed bumps including regions of ice-bed separation, is shown to melt basal ice internally in a narrow boundary layer at most centimeters thick. Before ice at the ice-bed interface can begin to melt, the heat input Q must exceed a critical value Q*. Q* increases rapidly with an increase in the difference ΔΡ between the nominal (global) overburden pressure and the magnitude of the (local) normal stress acting between the ice and bed or ice and water pocket. Because of the non-linear nature of the flow law, the thickness of the boundary layer decreases rapidly with increasing ΔΡ. The ice in the boundary layer is likely to be soft with a high water content. Under certain conditions, a regelation cycle may exist between the boundary layer and the water in a subglacial cavity. The boundary layer is sufficiently narrow that the processes can reach steady state while ice traverses subglacial low-pressure regions of length the order of 0.01–0.1 m. The regelation phenomenon may preserve or aid the formation of narrow debris-rich ice layers at the base of temperate glaciers.

Application of Medium Pressure UV Lamp for Wastewater Disinfection of Milk Production Industry

2006 ◽  
Vol 6 (4) ◽  
pp. 731-734 ◽  
Author(s):  
H. Kamani ◽  
F. Vaezi . ◽  
R. Nabizadeh . ◽  
A.R. Mesdaghinia . ◽  
M. Alimohammadi .
Keyword(s):  
Milk Production ◽  
Medium Pressure ◽  
Wastewater Disinfection ◽  
Uv Lamp ◽  
Production Industry
Sign in / Sign up

Export Citation Format

Share Document