scholarly journals Application of a Krypton-Chlorine Excilamp To Control Alicyclobacillus acidoterrestris Spores in Apple Juice and Identification of Its Sporicidal Mechanism

2020 ◽  
Vol 86 (11) ◽  
Author(s):  
Jun-Won Kang ◽  
Hak-Nyeong Hong ◽  
Dong-Hyun Kang
Keyword(s):  
Thermal Treatment ◽  
Apple Juice ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Alicyclobacillus Acidoterrestris ◽  
Content Type ◽  
Inactivation Mechanism ◽  
Uv Lamp ◽  
Uv Technology ◽  
Inactivation Effect

ABSTRACT The aim of this study was to investigate the sporicidal effect of a krypton-chlorine (KrCl) excilamp against Alicyclobacillus acidoterrestris spores and to compare its inactivation mechanism to that of a conventional UV lamp containing mercury (Hg). The inactivation effect of the KrCl excilamp was not significantly different from that of the Hg UV lamp for A. acidoterrestris spores in apple juice despite the 222-nm wavelength of the KrCl excilamp having a higher absorption coefficient in apple juice than the 254-nm wavelength of the Hg UV lamp; this is because KrCl excilamps have a fundamentally greater inactivation effect than Hg UV lamps, which is confirmed under ideal conditions (phosphate-buffered saline). The inactivation mechanism analysis revealed that the DNA damage induced by the KrCl excilamp was not significantly different (P > 0.05) from that induced by the Hg UV lamp, while the KrCl excilamp caused significantly higher (P < 0.05) lipid peroxidation incidence and permeability change in the inner membrane of A. acidoterrestris spores than did the Hg UV lamp. Meanwhile, the KrCl excilamp did not generate significant (P > 0.05) intracellular reactive oxygen species, indicating that the KrCl excilamp causes damage only through the direct absorption of UV light. In addition, after KrCl excilamp treatment with a dose of 2,011 mJ/cm2 to reduce A. acidoterrestris spores in apple juice by 5 logs, there were no significant (P > 0.05) changes in quality parameters such as color (L*, a*, and b*), total phenolic compounds, and DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical scavenging activity. IMPORTANCE Alicyclobacillus acidoterrestris spores, which have high resistance to thermal treatment and can germinate even at low pH, are very troublesome in the juice industry. UV technology, a nonthermal treatment, can be an excellent means to control heat-resistant A. acidoterrestris spores in place of thermal treatment. However, the traditionally applied UV sources are lamps that contain mercury (Hg), which is harmful to humans and the environment; thus, there is a need to apply novel UV technology without the use of Hg. In response to this issue, excilamps, an Hg-free UV source, have been actively studied. However, no studies have been conducted applying this technique to control A. acidoterrestris spores. Therefore, the results of this study, which applied a KrCl excilamp for the control of A. acidoterrestris spores and elucidated the inactivation principle, are expected to be utilized as important basic data for application to actual industry or conducting further studies.

scholarly journals Enhanced Antioxidant Activity for Apple Juice Fermented with Lactobacillus plantarum ATCC14917

Molecules ◽  
2018 ◽  
Vol 24 (1) ◽  
pp. 51 ◽  
Author(s):  
Zhongxi Li ◽  
Jing Teng ◽  
Yilu Lyu ◽  
Xiaoqian Hu ◽  
Yueliang Zhao ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Chemical Composition ◽  
Lactobacillus Plantarum ◽  
Apple Juice ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Phenolic Profile ◽  
Chemical Models ◽  
Phenolic And Flavonoid

The present study examined the effect of Lactobacillus plantarum ATCC14917 fermentation on the chemical composition and antioxidant activity of apple juice. Apple juice was fermented and examined of its antioxidant activity using chemical models and cellular antioxidant assay. Furthermore, the chemical composition of fermented apple juice was characterized by LC-MS/MS. Lactobacillus plantarum ATCC14917 fermentation showed an increase in DPPH and ABTS radical scavenging activity as well as cellular antioxidant activity of apple juice. However, fermentation decreased the total phenolic and flavonoid content. Subsequent LC-MS/MS analysis of the phenolic profile indicated that the content of 5-O-caffeoylquinic acid (5-CQA), quercetin, and phloretin with strong antioxidant activity was increased significantly after fermentation. The modified phenolic composition may contribute to the increased antioxidant activity of fermented apple juice. Our findings showed that Lactobacillus plantarum ATCC14917 fermentation may be an efficient way to enhance the bioavailability of phenolic compounds and to protect cells from oxidative stress.

Biologically active compounds released and extraction efficiency of cantaloupe juice treated with combination of Rapidase EX color and Accellerase

2017 ◽  
Vol 47 (5) ◽  
pp. 623-634 ◽  
Author(s):  
Phisut Naknaen
Keyword(s):  
Extraction Efficiency ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Value Added ◽  
Biologically Active Compounds ◽  
Biologically Active ◽  
Total Phenolic ◽  
Active Compounds ◽  
Antioxidant Power ◽  
Content Type

Purpose The purpose of this paper is to investigate the effects of concentrations of mixed enzymes (Rapidase EX Color and Accellerase) and incubation times on the release of biologically active compounds and the extraction efficiency of cantaloupe juice. Design/methodology/approach Different concentrations of the mixed enzymes (0.25-1.0 per cent) and incubation times (1-5 h) were used. The yield, clarity, bioactive compounds and antioxidant activities were investigated. Findings The total phenolic content, total flavonoid content, β-carotene, ascorbic acid content and antioxidant capacities as evaluated by 2, 2-diphenyl-1-picrylhydrazyl-radical scavenging activity and ferric reducing antioxidant power were approximately 6, 3.25, 3, 2.5, 3.5 and 3.0 times higher than the control samples after incubating with 0.5 per cent enzyme for 2 h (p < 0.05). This condition was recommended to promote the release of biologically active compounds and still provided a 32 per cent increase in the amount of the extracted juice. Practical implications Cantaloupe juice produced by enzymatic treatment could be claimed to be a source of health enhancing compounds. This work could be used as a guideline for the industry to develop high value-added functional drinks from cantaloupe. Originality/value This study evaluates for the first study on the application of Rapidase EX Color and Accellerase for improving the phytochemical compounds in cantaloupe juice. This technique could successfully extract the cantaloupe juice with biologically active nutrients containing beneficial health effects.

Fermented rice bran extract improves blood pressure and glucose in stroke-prone spontaneously hypertensive rats

2019 ◽  
Vol 49 (5) ◽  
pp. 844-853 ◽  
Author(s):  
Ardiansyah ◽  
Wahyudi David ◽  
Dody Dwi Handoko ◽  
Bram Kusbiantoro ◽  
Slamet Budijanto ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Oral Administration ◽  
Rice Bran ◽  
Spontaneously Hypertensive Rats ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Hypertensive Rats ◽  
Content Type ◽  
Spontaneously Hypertensive

PurposeThe purpose of this study was to evaluate thein vitroandin vivoeffects of three varieties of Indonesian fermented rice bran (RB) (Inpari 6, Inpari 30 and Inpara 1).Design/methodology/approachThree types of RB were fermented usingRhizopus oligosporus. The total phenolic content (TPC) was determined using the Folin–Ciocalteau method, and antioxidant activity was analyzed by measuring the 2,2-diphenyl-1-picrylhydrazyl free radical scavenging activity (RSA). Forin vivoanalyses, one week after acclimatization, stroke-prone spontaneously hypertensive rats (n= 4, 12 weeks of age) were divided into two groups and orally administered fermented RB (40 mg/kg body weight) or distilled water as a control after 16 h of fasting. Blood pressure (BP) was measured before and 2, 4 and 6 h after administration with a BP monitor without warming. Six days later, the rats were subject to the same procedure and sacrificed after 6 h of oral administration. Blood was collected and the plasma was separated to measure nitric oxide, glucose and insulin levels.FindingsThe highest TPC and RSA of fermented RB were obtained from Inpari 30 after incubation for 72 h (260.33 ± 0.39 mg GAE/100 g dry basis and 83.71 ± 0.61 per cent), respectively. Furthermore, single oral administration of fermented RB improved BP (p< 0.05) and glucose metabolism after 6 h of administration (p< 0.05).Originality/valueThis is the first study to evaluate the effects of fermented RB on improving high BP and glucose profiles by using a spontaneously hypertensive animal model.

Extrusion and solid-state fermentation with Aspergillus oryzae on the phenolic compounds and radical scavenging activity of pecan nut (Carya illinoinensis) shell

2021 ◽  
Vol ahead-of-print (ahead-of-print) ◽  
Author(s):  
Juliana Villasante ◽  
Johanan Espinosa-Ramírez ◽  
Esther Pérez-Carrillo ◽  
Erick Heredia-Olea ◽  
MariaPilar Almajano
Keyword(s):  
Solid State ◽  
Phenolic Compounds ◽  
Aspergillus Oryzae ◽  
Solid State Fermentation ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Content Type

PurposeSolid-state fermentation (SSF) has been highlighted as an alternative to obtain valuable compounds using agro-industrial wastes as a substrate. The present study evaluated the impact of extrusion combined with SSF on the production of phenolic compounds and their antioxidant activity using pecan nut shell (PWS) as a substrate.Design/methodology/approachPWS and extruded pecan nut shell (PWSE) were fermented for 120 h at 30°C using Aspergillus oryzae (A. oryzae). Samples were withdrawn from incubator at 6 h, 12 h and then every 12 h until 120 h fermentation. PWS and PWSE samples were extracted. The total phenolic content (TPC) and radical scavenging activity (RSA) extracts were characterized from the resulting extracts.FindingsThe use of PWSE yielded higher A. oryzae biomass and at a higher rate after 120 h of fermentation (PWS 75.74% vs PWSE 87.50%). In general, the TPC and the RSA increased with fermentation time. However, the PWSE yielded significantly higher (p < 0.05) TPC and RSA values after SSF in comparison to the nonextruded PWS. Condensed tannins showed different trends depending on the fermented substrate. Overall, results showed that the extrusion pretreatment joint with the SSF represents a good alternative to raise the phenolic content and antiradical activity of lignocellulosic materials such as PWSs.Originality/valueThis study offers valuable information that may be used by the pecan walnut industry to valorize the shell coproduct as a substrate to produce functional ingredients or fungal enzymes.

Novel improvement of bioactive microencapsulated textile products using brown seaweed for healthcare applications

2017 ◽  
Vol 29 (2) ◽  
pp. 200-214 ◽  
Author(s):  
M. Janarthanan ◽  
M. Senthil Kumar
Keyword(s):  
Antibacterial Activity ◽  
Brown Algae ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Antimicrobial Properties ◽  
Brown Seaweed ◽  
Total Phenolic ◽  
Human Beings ◽  
Bacterial Reduction ◽  
Content Type

Purpose The healthcare and hygiene textiles are gaining more importance for their eco-friendly and effective antimicrobial properties that have become essential to safeguard human beings from harmful microorganisms. The fabrics finished with chemical-based antimicrobial agents lead to environmental issues and are harmful to human beings. The paper aims to discuss these issues. Design/methodology/approach The present investigation is to develop a fabric with antioxidant and antimicrobial properties using the extracts of brown algae. Antimicrobial property has been imparted to the cotton fabric using microcapsules of brown seaweed extracts using the pad-dry-cure method. The presence of bioactive compounds and antioxidant activities of brown algae extracts was evaluated using gas chromatography-mass spectrometry and 2, 2-diphenyl-1-picrylhydrazyl radical scavenging technique, respectively. The total phenolic content of the seaweed extract was determined by the Folin-Ciocalteu method. The minimum bactericidal concentration and minimum inhibitory concentration methods were used to determine the antibacterial activity of the bacterial reduction percentage and parallel streak methods were used evaluate the antibacterial activity of seaweed-treated fabrics. Findings The methanol fraction of the treated fabric had the highest antioxidant activity (42.5+1.21 per cent), because the higher phenolic content traps the reactive oxygen species and develops the cells present in the skin. The results show that the lower inhibition (250 µg/mL) and bactericidal concentrations (1,000 µg/mL) possess higher antibacterial activity. The results also show that the treated fabric possess higher bacterial reduction of 96 per cent and higher zone of inhibition against Escherichia Coli and Staphylococcus Aureus which was about 35 mm and 40 mm. The air permeability, bending length and the wicking behaviour of the treated fabric were slightly reduced, but it has good bursting strength compared with the untreated fabric. Originality/value Such treated fabric is used for making wound dressing, surgical gowns, antibacterial socks and gauze bandage products in healthcare and hygiene textiles.

Antioxidant properties and consumer acceptability of pearl millet – tiger nut biscuits

2015 ◽  
Vol 45 (6) ◽  
pp. 818-828 ◽  
Author(s):  
Olufunmilayo Sade Omoba ◽  
Oluwadamilola Olamiiposi Dada ◽  
Sule Ola Salawu
Keyword(s):  
Pearl Millet ◽  
Phenolic Content ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Consumer Acceptability ◽  
Potential Health ◽  
Content Type ◽  
Flour Blends

Purpose – This paper aims to focus on the antioxidant properties and consumer acceptability of biscuits produced from pearl millet (PM)–tiger nut (TN) composite blends, with the aim of encouraging the use of these under-utilised food crops in producing value-added products with nutraceutical potential. Design/methodology/approach – PM grains and TN seeds were processed into flour and blended at ratios: 15:85 (A), 20:80 (B), 25:75 (C), 30:70 (D), 35:65 (E), 40:60 (F), 45:55 (G), of PM to TN using response surface methodology. The antioxidant properties of the biscuits produced from the flour blends were determined using standard methods, and data obtained were subjected to analysis of variance (ANOVA); differences of means were separated using Duncan multiple range test. The biscuits were subjected to sensory evaluation using 50 untrained panellist and results statistically analysed. Findings – The total phenolic content (TPC-mg/GAE) of PM–TN flour blends ranged from 2.65-4.95 (A-G), with an average TPC of 3.85. In PM–TN biscuits, total phenolic values ranged from 1.20-3.42 (A-G), with an average of 2.39. The 2,2′-azino-bis(3-ethyl benzothiazoline-6-sulfonic acid) diammonium salt (ABTS) radical scavenging activities (μmolTEAC/g) of PM–TN flour blends ranged from 26.7-36.4 (A-G), and the values for biscuits ranged from 28.5-38.6 (A-G). There was a highly significant correlation, r = 0.811(p < 0.001) and r = 0.913 (p < 0.001) between phenolic content and antioxidant activity for flour and biscuit, respectively. The effect of in vitro digestion of biscuits on the antioxidant assays viz-a-viz: TPC and ABTS radical scavenging activities were also investigated. The order of phenolic release was enzymic > aqueous > ethanolic digest for TPC, while for ABTS, the order of release was enzymic > ethanolic > aqueous. The highest values for antioxidant properties were found in the simulated in vitro enzyme digested biscuits. Consumer acceptability revealed that the overall acceptability scores of the biscuits were not significantly different except for biscuit sample G. Originality/value – The paper has demonstrated the antioxidant potentials and consumer acceptability of PM–TN biscuits. It also shows the effect of in vitro digestion on the antioxidant activities of the biscuit and its potential health benefit, as a source of antioxidant.

scholarly journals Rapid Pasteurization of Apple Juice Using a New Ultrasonic Reactor

Foods ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 801
Author(s):  
Zahra Moaddabdoost Baboli ◽  
Leonard Williams ◽  
Guibing Chen
Keyword(s):  
Antioxidant Activity ◽  
Apple Juice ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ultrasound Treatment ◽  
Plate Count ◽  
Total Phenolic ◽  
E Coli ◽  
Log Reduction ◽  
Dpph Radical Scavenging

A new ultrasonic reactor was used to rapidly inactivate Escherichia coli and Staphylococcus aureus in apple juice. It was found that high pulp content made ultrasound less lethal to S. aureus, while it had no significant effect on E. coli. When the pulp free apple juice was ultrasonically processed, the 5-log reduction time was 35 s for E. coli at 60 °C and 30 s for S. aureus at 62 °C. Ultrasound treatment had no significant effect on antioxidant activity determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, but it significantly increased the total phenolic content. The treatment also resulted in more stable juice with higher uniformity. During 28 d of storage at 4 °C, the total plate count in apple juice ultrasonically treated at 60 °C for 35 s remained around 1.00 log CFU/mL, whereas it was nearly zero for a stronger ultrasound treatment at 62 °C for 30 s. These values were much lower than those in the untreated one, which increased from 3.65 log CFU/mL to 8.36 log CFU/mL during the storage. At the end of the storage, the control and thermally treated apple juice lost almost 70% of antioxidant activity, whereas the ultrasonically treated juice only lost 20–40%.

scholarly journals Increased Resistance ofSalmonella entericaSerovar Typhimurium andEscherichia coliO157:H7 to 222-Nanometer Krypton-Chlorine Excilamp Treatment by Acid Adaptation

2019 ◽  
Vol 85 (6) ◽  
Author(s):  
Jun-Won Kang ◽  
Dong-Hyun Kang
Keyword(s):  
Cell Membrane ◽  
Quality Indicators ◽  
Apple Juice ◽  
Radical Scavenging Activity ◽  
Saturated Fatty Acids ◽  
Radical Scavenging ◽  
Attractive Alternative ◽  
Juice Quality ◽  
Acid Adaptation ◽  
Content Type

ABSTRACTIn this study, we examined the change in resistance ofSalmonella entericaserovar Typhimurium andEscherichia coliO157:H7 to 222-nm krypton-chlorine (KrCl) excilamp treatment as influenced by acid adaptation and identified a mechanism of resistance change. In addition, we measured changes in apple juice quality indicators, such as color, total phenols, and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity, during treatment. Non-acid-adapted and acid-adapted pathogens were induced by growing the cells in tryptic soy broth without dextrose (TSB w/o D) at pH 7.3 and in TSB w/o D at pH 5.0 (adjusted with HCl), respectively. For the KrCl excilamp treatment, acid-adapted pathogens exhibited significantly (P < 0.05) higher D5dvalues, which indicate dosages required to achieve a 5-log reduction, than those for non-acid-adapted pathogens in both commercially clarified apple juice and phosphate-buffered saline (PBS), and the pathogens in the juice showed significantly (P < 0.05) higher D5dvalues than those for pathogens in PBS because of the UV-absorbing characteristics of apple juice. Through mechanism identification, it was found that the generation of lipid peroxidation in the cell membrane, inducing cell membrane destruction, was significantly (P < 0.05) lower in acid-adapted cells than in non-acid-adapted cells for the same amount of reactive oxygen species (ROS) generated at the same dose because the ratio of unsaturated to saturated fatty acids (USFA/SFA) in the cell membrane was significantly (P < 0.05) decreased as a result of acid adaptation. Treated apple juice showed no significant (P > 0.05) difference in quality indicators compared to those of untreated controls during treatment at 1,773 mJ/cm2.IMPORTANCEThere is a need for novel, mercury-free UV lamp technology to replace germicidal lamps containing harmful mercury, which are routinely utilized for UV pasteurization of apple juice. In addition, consideration of the changes in response to antimicrobial treatments that may occur when pathogens are adapted to the acid in an apple juice matrix is critical to the practical application of this technology. Based on this, an investigation using 222-nm KrCl excilamp technology, an attractive alternative to mercury lamps, was conducted. Our study demonstrated increased resistance to 222-nm KrCl excilamp treatment as pathogens adapted to acids, and this was due to changes in reactivity to ROS with changes in the fatty acid composition of the cell membrane. Despite increased resistance, the 222-nm KrCl excilamp achieved pathogen reductions of 5 log or more at laboratory scale without affecting apple juice quality. These results provide valuable baseline data for application of 222-nm KrCl excilamps in the apple juice industry.

GC‐MS and antioxidant capacity analyses of cowpea seeds oils

2013 ◽  
Vol 43 (2) ◽  
pp. 116-127 ◽  
Author(s):  
Muhammad Nadeem Asghar ◽  
Javaid Akhtar ◽  
Muhammad Shafiq ◽  
Iram Nadeem ◽  
Muhammad Ashfaq ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Radical Cation ◽  
Vegetable Oils ◽  
Chemical Constituents ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Content Type ◽  
Percent Inhibition ◽  
White Star

PurposeThe cowpea plant, being affordable and protein‐rich, is considered poor man's meat. The aim of this paper is to undertake a detailed investigation regarding in vitro total antioxidant capacity (TAC) and chemical constituents of the vegetable oils from seeds of this plant, taking its nutritional value into consideration.Design/methodology/approachVegetable oils of different indigenous cowpea varieties were obtained using soxhelt extraction assembly and subjected to GC‐MS analyses and various antioxidant assays including 2,2′‐azinobis(3‐ethylbenzothiazoline‐6‐sulpohonic acid) (ABTS) radical cation scavenging, ferr, 2.2′‐diphenyl‐1‐picrylhydrazil (DPPH) radical scavenging, total phenolic contents (TPC), lipid peroxidation inhibition, and iron chelation activity.FindingsVarious chemical constituents including different hydrocarbons, tocopherols, ketones, fatty esters, estragole and cedrene were identified. TPCs were found to be 5.439, 5.7279, 7.6126, 6.7573 and 10.0591 mg/L gallic acid equivalent for S.A. Dandy, Elite, White Star, CP‐386 and FBD Rawan varieties, respectively. Employing ABTS radical decoloration assay a significant linear correlation (R2=0.997, 0.996, 0.997, 0.996 and 0.997 for S.A. Dandy, Elite, White Star, CP‐386 and FBD Rawan varieties, respectively) was found between the percent inhibition of ABTS radical cation and the amount of vegetable oils. The percent inhibition of the Fe(II)‐Ferrozine complex formation was found to be 29.45, 53.76, 82.91, 86.59 and 57.87 for the same varieties, respectively.Originality/valueGC‐MS and standard in vitro antioxidative capacity analyses data clearly demonstrated the potency of the cowpea as antioxidant and radical scavenger plant which may be used as a good source of natural antioxidants. The plant seeds may prove a better and cost‐effective substitute of expensive food items.

Phytochemical and Pharmacological Activity of Myristica fragrans Houtt (Myristicaceae)

2017 ◽  
Vol 9 (01) ◽  
Author(s):  
Md Arifur Rahman Chowdhury ◽  
Manirujjaman . ◽  
Md Mazedul Haq
Keyword(s):  
Acetic Acid ◽  
Phenolic Content ◽  
Methanolic Extract ◽  
Radical Scavenging ◽  
Antioxidant Property ◽  
Free Radical Scavenging ◽  
Total Phenolic ◽  
Writhing Test ◽  
Myristica Fragrans ◽  
Myristica Fragrans Houtt

Objective: Myristica fragrans Houtt commonly used as traditional medicine for alleviating of various disorders. The purpose of our study was to map out the in vitro antioxidant property and in vivo anti-hyperglycemic and analgesic effect of the methanolic extract of Myristica fragrans Houtt. (Seed and mace) (Myristicaceae) on Swiss albino mice. Methods: The processed powder of Myristica fragrans Houtt (seed and mace) were subjected to methanolic extraction by soxhlet filtration methods, and the desiccated extract was used for screening of antioxidant by DPPH free radical scavenging assessment as well as total phenolic content by using folin-ciocalteu reagent.Anti-hyperglycemic effect and analgesic action tested through alloxan induced antidiabetics test and acetic acid-tempted writhing test on mice. Results: In DPPH free radical scavenging assessment, free radicals neutralization expressed as % of inhibition 49.69±0.06% also by IC50 values as 68.43 µg/ml surmise middle level of antioxidant property. The total phenolic content expressed as 186.25 mg/g equivalent of gallic acid indicates, active phenolic content. Oral administration of 200 and 400 mg/kg of extract dose and reference drug vildagliptin (50 mg/kg) for the duration of the 4-day study period, and initiated % of inhibition the blood glucose level measured as 22.48%, 44.78% and 62.02% regard as the significant anti-hyperglycemic properties. The analgesic activity was investigated by using the acetic acid-induced writhing test in mice, at the dose of 200 mg/kg body and 400mg/kg weight, and resulting 50.4% and 68.10% correspondingly, which was considerably significant with a standard drug. Conclusion: The present study suggests that methanolic extract of seed and mace of Myristica fragrans Houtt can manage moderate oxidative stress as well as perform the painkilling action. Besides, prolong medication may enhance the new dimension of anti-hyperglycemic activity.

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