scholarly journals Antibiotic Susceptibility Testing of Gram-Negative Nonfermentative Bacilli

1975 ◽  
Vol 7 (4) ◽  
pp. 400-412 ◽  
Author(s):  
K. A. Ruddell ◽  
C. R. Anselmo
Keyword(s):  
Antibiotic Susceptibility ◽  
Susceptibility Testing ◽  
Antibiotic Susceptibility Testing ◽  
Gram Negative

Querying Automated Antibiotic Susceptibility Testing Instruments: A Novel Population-Based Active Surveillance Method for Multidrug-Resistant Gram-Negative Bacilli

2014 ◽  
Vol 35 (4) ◽  
pp. 336-341 ◽  
Author(s):  
Jessica Reno ◽  
Calista Schenck ◽  
Janine Scott ◽  
Leigh Ann Clark ◽  
Yun F. (Wayne) Wang ◽  
...  
Keyword(s):  
Active Surveillance ◽  
Antibiotic Susceptibility ◽  
Susceptibility Testing ◽  
Multidrug Resistant ◽  
Population Based ◽  
Antibiotic Susceptibility Testing ◽  
Emerging Infections ◽  
Gram Negative ◽  
Ongoing Surveillance ◽  
Testing Instruments

Objective.To describe the implementation of a population-based surveillance system for multidrug-resistant gram-negative bacilli (MDR-GNB).Design.Population-based active surveillance by the Georgia Emerging Infections Program.Setting.Metropolitan Atlanta, starting November 2010.Patients.Residents with MDR-GNB isolated from urine or a normally sterile site culture.Methods.Surveillance was implemented in 3 phases: (1) surveying laboratory antibiotic susceptibility testing practices, (2) piloting surveillance to estimate the proportion of GNB that were MDR, and (3) maintaining ongoing active surveillance for carbapenem-nonsusceptible Enterobacteriaceae and Acinetobacter baumannii using the 2010 Clinical and Laboratory Standards Institute (CLSI) breakpoints. Pilot surveillance required developing and installing queries for GNB on the 3 types of automated testing instruments (ATIs), such as MicroScan, in Atlanta's clinical laboratories. Ongoing surveillance included establishing a process to extract data from ATIs consistently, review charts, manage data, and provide feedback to laboratories.Results.Output from laboratory information systems typically used for surveillance would not reliably capture the CLSI breakpoints, but queries developed for the 3 ATIs did. In November 2010, 0.9% of Enterobacteriaceae isolates and 35.7% of A. baumannii isolates from 21 laboratories were carbapenem nonsusceptible. Over a 5-month period, 82 Enterobacteriaceae and 59 A. baumannii were identified as carbapenem nonsusceptible.Conclusions.Directly querying ATIs, a novel method of active surveillance for MDR-GNB, proved to be a reliable, sustainable, and accurate method that required moderate initial investment and modest maintenance. Ongoing surveillance is critical to assess the burden of and changes in MDR-GNB to inform prevention efforts.

scholarly journals Antibiotic Susceptibility Testing of the Gram-Negative Bacteria Based on Flow Cytometry

2016 ◽  
Vol 7 ◽  
Author(s):  
Claude Saint-Ruf ◽  
Steve Crussard ◽  
Christine Franceschi ◽  
Sylvain Orenga ◽  
Jasmine Ouattara ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Antibiotic Susceptibility ◽  
Susceptibility Testing ◽  
Gram Negative Bacteria ◽  
Antibiotic Susceptibility Testing ◽  
Gram Negative

scholarly journals Rapid and accurate direct antibiotic susceptibility testing of blood culture broths using MALDI Sepsityper combined with the BD Phoenix automated system

2014 ◽  
Vol 63 (12) ◽  
pp. 1590-1594 ◽  
Author(s):  
Briony Hazelton ◽  
Lee C. Thomas ◽  
Thomas Olma ◽  
Jen Kok ◽  
Matthew O’Sullivan ◽  
...  
Keyword(s):  
Blood Culture ◽  
Antibiotic Susceptibility ◽  
Susceptibility Testing ◽  
Turnaround Time ◽  
Automated System ◽  
Antibiotic Susceptibility Testing ◽  
Gram Negative ◽  
Cell Pellet ◽  
Novel Method ◽  
Susceptibility Tests

Antibiotic susceptibility testing with the BD Phoenix system on bacterial cell pellets generated from blood culture broths using the Bruker MALDI Sepsityper kit was evaluated. Seventy-six Gram-negative isolates, including 12 with defined multi-resistant phenotypes, had antibiotic susceptibility testing (AST) performed by Phoenix on the cell pellet in parallel with conventional methods. In total, 1414/1444 (97.9 %) of susceptibility tests were concordant, with only 1 (0.07 %) very major error. This novel method has the potential to reduce the turnaround time for AST results by up to a day for Gram-negative bacteraemias.

scholarly journals Rapid phenotypic stress-based microfluidic antibiotic susceptibility testing of Gram-negative clinical isolates

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Maxim Kalashnikov ◽  
Marc Mueller ◽  
Christine McBeth ◽  
Jean C. Lee ◽  
Jennifer Campbell ◽  
...  
Keyword(s):  
Antibiotic Susceptibility ◽  
Susceptibility Testing ◽  
Clinical Isolates ◽  
Antibiotic Susceptibility Testing ◽  
Gram Negative
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