scholarly journals Antimicrobial Susceptibility of Neisseria gonorrhoeae in Fukuoka City, Japan, in the Early 1980s and 1997–1998: Emergence of High-Level Fluoroquinolone Resistance

1999 ◽  
Vol 43 (3) ◽  
pp. 722-723 ◽  
Author(s):  
Masatoshi Tanaka ◽  
Hiroshi Nakayama ◽  
Intetsu Kobayashi ◽  
Seiji Naito
Keyword(s):  
Neisseria Gonorrhoeae ◽  
Antimicrobial Susceptibility ◽  
Fluoroquinolone Resistance ◽  
High Level

scholarly journals Antimicrobial Susceptibility of Neisseria gonorrhoeae Isolates in Baltimore, Maryland, 2016: The Importance of Sentinel Surveillance in the Era of Multi-Drug-Resistant Gonorrhea

Antibiotics ◽  
2018 ◽  
Vol 7 (3) ◽  
pp. 77 ◽  
Author(s):  
Johan Melendez ◽  
Justin Hardick ◽  
Mathilda Barnes ◽  
Kathleen Page ◽  
Charlotte Gaydos
Keyword(s):  
Neisseria Gonorrhoeae ◽  
Antimicrobial Susceptibility ◽  
Tetracycline Resistance ◽  
Sentinel Surveillance ◽  
Test Method ◽  
Nucleic Acid Amplification ◽  
Drug Resistant ◽  
Surveillance Programs ◽  
Resistance To Penicillin ◽  
High Level

The increasing rates of gonorrhea infections and the global emergence and spread of multi-drug-resistant Neisseria gonorrhoeae (NG) threaten the successful management of gonorrhea. In the era of nucleic acid amplification tests (NAATs), surveillance projects are urgently needed to monitor prevalence and trends in the antimicrobial susceptibility of NG. In this study, we retrospectively determined the susceptibility profile of NG isolates to previously and currently prescribed antimicrobials. NG isolates collected in Baltimore, Maryland between January and October 2016 were evaluated by the E-test method and/or molecular methods for susceptibility to ceftriaxone, azithromycin, ciprofloxacin, tetracycline, gentamicin, and penicillin. One-hundred and forty-three NG isolates from African-American males (98.6%), primarily heterosexual (88.8%), ranging in age from 15 to 69 years of age were included in the study. Ciprofloxacin resistance was observed in 44.1% of isolates. Plasmid-mediated resistance to penicillin and tetracycline resistance was detected in 22.4% and 10.1% of isolates, respectively. Three isolates (2.1%) displayed high-level resistance to azithromycin (minimum inhibitory concentration (MIC) > 256 μg/mL). Forty-three percent of isolates were resistant or had decreased susceptibility to three antimicrobials (ciprofloxacin, tetracycline, and penicillin). All isolates were susceptible to ceftriaxone and gentamicin. Overall, the epidemiology of antimicrobial resistant NG in Baltimore continues to evolve, and the emergence of azithromycin resistance in this population emphasizes the need for continued sentinel surveillance programs to monitor susceptibility trends and aid in treatment recommendations.

scholarly journals Antimicrobial Susceptibility Profile of Gonococcal Isolates Obtained from Men Presenting with Urethral Discharge: Implication for National Syndromic Treatment guideline

2019 ◽  
Author(s):  
Surafel Fentaw Dinku ◽  
Rajiha Abubeker ◽  
Nega Asamene ◽  
Meseret Assefa ◽  
Eyasu Tigabu
Keyword(s):  
Neisseria Gonorrhoeae ◽  
Antimicrobial Susceptibility ◽  
Antimicrobial Agents ◽  
Treatment Guideline ◽  
Etiologic Agent ◽  
Fluoroquinolone Resistance ◽  
Diffusion Method ◽  
Public Health Importance ◽  
Urethral Discharge ◽  
Treatment Regimens

Abstract Background: Neisseria gonorrhoeae (gonococcus) is one of etiologic agent for sexually transmitted diseases with high global significant public health importance. The treatment regimens for gonorrhea have changed frequently over the past few decades due to the organism’s propensity for developing antibiotic resistance. This study was aimed to investigate quinolones, third-generation cephalosporins and other relevant antimicrobials susceptibility patterns of N. gonorrhoeae so that the national treatment regimen could be revised based on the information generated from this study. Methods: Urethral discharge specimens were cultured on Modified Thayer Martín media and suspected gonococcal colonies were confirmed using Oxidase, Superoxol tests followed by commercial identification kit (API-NHR). Antimicrobial susceptibility testing was performed by Kirby-Bauer disc diffusion method using ciprofloxacin(5ug), ceftriaxone (30ug), cefixime(5ug), cefoxitin (30 ug), and spectinomycin (100 ug), on enriched GC agar. Minimum Inhibitory Concentration (MIC) was also done using concentration gradient strips (E-test) of the same antimicrobial agents. Results: The prevalence of gonococcal isolates from the current study was 69%. Out of the 361 gonococcal isolates, close to 68% were fluoroquinolone non susceptible with 60% resistant and 7% intermediate status. However, there was no detection of ceftriaxone non susceptible gonococcal isolates. The isolates also showed reduced non susceptible to spectinomycin and cefoxitin. Conclusion: The prevalence of gonococcal isolates from the current study was high. There was a high level of fluoroquinolone resistance in gonococcal isolates recovered in this study. Key words: Neisseria gonorrhoeae, Ciprofloxacin, Ceftriaxone MIC, Ethiopia

scholarly journals Antimicrobial susceptibility of Neisseria gonorrhoeae in Barcelona during a five-year period, 2013 to 2017

2020 ◽  
Vol 25 (42) ◽  
Author(s):  
Paula Salmerón ◽  
Belén Viñado ◽  
Rachid El Ouazzani ◽  
Marta Hernández ◽  
María Jesús Barbera ◽  
...  
Keyword(s):  
Neisseria Gonorrhoeae ◽  
Antimicrobial Susceptibility ◽  
Susceptibility Testing ◽  
Testing Time ◽  
Susceptibility Data ◽  
European Committee ◽  
Clinical Breakpoints ◽  
High Level ◽  
Resistance Rates ◽  
Level Resistance

Introduction Increasing rates of antimicrobial resistance in Neisseria gonorrhoeae cause problems for treating gonorrhoea. Aim This observational study aimed to describe isolates from all patients found infected with N. gonorrhoeae, in Barcelona, Spain, between 2013 and 2017, and with available antimicrobial susceptibility data. Methods Minimum inhibitory concentrations (MICs) of penicillin (PEN), cefixime (CFM), ceftriaxone (CRO), azithromycin (AZM), ciprofloxacin (CIP), spectinomycin (SPT), fosfomycin (FOF) and gentamicin (GEN) were determined by E-test. Susceptibility was assessed using clinical breakpoints from the European Committee on Antimicrobial Susceptibility Testing. Time trends for PEN, CFM, AZM and CIP were investigated using logistic regression. Results Of 1,979 patients with infection (2,036 isolates), 1,888 (95.4%) were men. Patient median age was 32 years. The proportions of isolates resistant to extended-spectrum cephalosporins were low, with 0.3% (5/1,982) resistant to CRO and 4.9% (98/1,985) to CFM. AZM resistance prevalence was 2.7% (52/1,981), including 16 isolates detected in 2016 and 2017, with high-level resistance. For CIP, 51.3% (1,018/1,986) of isolates were resistant, and for PEN, 20.1% (399/1,985). All isolates were susceptible to SPT. MIC50 and MIC90 values of GEN were 4 and 6 mg/L and of FOF 12 and 24 mg/L, respectively. Between 2013 and 2017, PEN and CFM resistance rates each decreased from 28.1% (92/327) to 12.2% (70/572) and from 8.3% (27/327) to 4.4% (25/572) (p ≤ 0.0073). In contrast, AZM resistance prevalence appeared to increase from 1.5% in 2014 (5/340) to 3.0% (17/572) in 2017. No trend was identified for CIP. Conclusion Antimicrobial susceptibility surveillance is important to timely detect new phenotypes and trends.

scholarly journals Molecular Basis of High-Level Ciprofloxacin Resistance in Neisseria gonorrhoeae Strains Isolated in Denmark from 1995 to 1998

2001 ◽  
Vol 45 (1) ◽  
pp. 117-123 ◽  
Author(s):  
Xiaohong Su ◽  
Inga Lind
Keyword(s):  
Neisseria Gonorrhoeae ◽  
Pulsed Field Gel Electrophoresis ◽  
Fluoroquinolone Resistance ◽  
Resistant Clone ◽  
In Vitro Susceptibility ◽  
Mutation Pattern ◽  
Resistant Strains ◽  
Ciprofloxacin Resistance ◽  
High Level

ABSTRACT In Denmark surveillance of the in vitro susceptibility to ciprofloxacin of Neisseria gonorrhoeae was established in 1990. The proportion of N. gonorrhoeae strains with decreased susceptibility or resistance to ciprofloxacin (MIC ≥ 0.06 μg/ml) was low (0.3 to 2.3%) up to 1995. Between 1995 and 1998 the rate of less-susceptible and resistant strains rose from 6.9 to 13.2%. Among ciprofloxacin-resistant strains (MIC ≥ 1 μg/ml), 81% were highly resistant (MIC ≥ 4 μg/ml). Thirty-fiveN. gonorrhoeae strains (40 isolates) for which ciprofloxacin MICs were 4 to 32 μg/ml were investigated for the frequency and patterns of mutations within the gyrA andparC genes. The quinolone resistance-determining regions of the gyrA and parC genes were amplified by PCR, and the amplicons were directly sequenced. Alterations at Ser-91 and Asp-95 in GyrA and a single or double alteration in ParC were identified in 32 strains (91%). Ser-91-to-Phe and Asp-95-to-Gly alterations in GyrA were detected in 28 strains (80%). The most common ParC alteration, Asp-86 to Asn, was found in 19 strains (54%). The strains were analyzed for genetic relationship by pulsed-field gel electrophoresis (PFGE). The analysis showed that nine strains with the same mutation pattern in the gyrA and parCgenes, originating from different geographical areas over 3 years, had the same PFGE patterns after SpeI as well asNheI digestion (only one strain with one band difference in the NheI pattern), suggesting that a resistant clone had spread worldwide. The results from this study strongly suggest that double gyrA mutations plus a parCmutation(s) play an important role in the development of high-level fluoroquinolone resistance in N. gonorrhoeae.

scholarly journals Antimicrobial Resistance of Neisseria gonorrhoeae and High Prevalence of Ciprofloxacin-Resistant Isolates in Japan, 1993 to 1998

2000 ◽  
Vol 38 (2) ◽  
pp. 521-525 ◽  
Author(s):  
Masatoshi Tanaka ◽  
Hiroshi Nakayama ◽  
Masashi Haraoka ◽  
Takeshi Saika ◽  
Intetsu Kobayashi ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Neisseria Gonorrhoeae ◽  
Susceptibility Testing ◽  
Tetracycline Resistance ◽  
Penicillin Resistance ◽  
Fluoroquinolone Resistance ◽  
Amino Acid Substitutions ◽  
High Prevalence ◽  
Ciprofloxacin Resistance ◽  
High Level

To assess the antimicrobial resistance of Neisseria gonorrhoeae isolated from 1993 through 1998 in Japan, susceptibility testing was conducted on 502 isolates. Selected isolates were characterized by auxotype and analysis for mutations within the quinolone resistance-determining region (QRDR) in the gyrAand parC genes, which confer fluoroquinolone resistance on the organism. Plasmid-mediated penicillin resistance (penicillinase-producing N. gonorrhoeae) decreased significantly from 1993–1994 (7.9%) to 1997–1998 (2.0%). Chromosomally mediated penicillin resistance decreased from 1993–1994 (12.6%) to 1995–1996 (1.9%) and then increased in 1997–1998 (10.7%). Chromosomally mediated tetracycline resistance decreased from 1993–1994 (3.3%) to 1997–1998 (2.0%), and no plasmid-mediated high-level tetracycline resistance was found. Isolates with ciprofloxacin resistance (MIC ≥ 1 μg/ml) increased significantly from 1993–1994 (6.6%) to 1997–1998 (24.4%). The proline-requiring isolates were less susceptible to ciprofloxacin than the prototrophic or arginine-requiring isolates. Ciprofloxacin-resistant isolates contained three or four amino acid substitutions within the QRDR in the GyrA and ParC proteins.

Sign in / Sign up

Export Citation Format

Share Document