scholarly journals Mycobacteriocidal Action of Exogenous Nitric Oxide

1999 ◽  
Vol 43 (2) ◽  
pp. 403-405 ◽  
Author(s):  
Richard Long ◽  
Bruce Light ◽  
James A. Talbot
Keyword(s):  
Nitric Oxide ◽  
Mycobacterium Tuberculosis ◽  
Pulmonary Tuberculosis ◽  
Analysis Of Variance ◽  
Time Dependent ◽  
Low Concentrations ◽  
Exogenous Nitric Oxide

ABSTRACT We tested the hypothesis that exposure of extracellularMycobacterium tuberculosis to low concentrations (<100 ppm) of nitric oxide (NO) for short periods (24 h or less) will result in microbial killing. We observed that NO had both dose- and time-dependent cidal effects that were very significant by two-way analysis of variance (F ratios of 13.4 [P< 0.001] and 98.1 [P < 0.0001], respectively). Conceivably, extracellular bacilli in patients with pulmonary tuberculosis might be vulnerable to exogenous NO.

Effects of Exogenous Nitric Oxide at Different Concentrations on the Photosynthesis of Pinus koraiensis Sieb. Et Zucc. Seedlings

2011 ◽  
Vol 183-185 ◽  
pp. 422-426
Author(s):  
Xiao Xue Wei ◽  
De Wen Li ◽  
Jing Hua Yu ◽  
Yuan Gang Zu
Keyword(s):  
Nitric Oxide ◽  
Pinus Koraiensis ◽  
Photosynthetic Parameters ◽  
Gas Exchange Parameters ◽  
No Donor ◽  
Chlorophyll Contents ◽  
Compensation Irradiance ◽  
Low Concentrations ◽  
Exogenous Nitric Oxide ◽  
Exchange Parameters

In the study, the gas exchange parameters and chlorophyll contents in the needles of Pinus koraiensis Sieb. et Zucc. exposed to exogenous nitric oxide (NO) were determined. The Pinus koraiensis Sieb. et Zucc. seedlings were treated with sodium nitroprusside (SNP), a NO donor, at 5 different concentrations ranging from 0 mM to 1 mM. The result showed that at different concentrations exogenous NO had different effects on the photosynthetic parameters and photosynthetic pigments. The content of chlorophyll was maximal in the treatment with SNP at 0.01 mM. At low concentrations (0.1 mM) SNP significantly increased the photosynthetic rate (PN) and decreased the Respiration rate (RD) and Compensation irradiance (IC) of the needles (p<0.05). The experiment proved that at low concentrations exogenous NO signals increased the photosynthesis of Pinus koraiensis Sieb. et Zucc. seedlings. The present results suggested that the lower concentrations SNP might decrease the level of reactive oxygen species (ROS) and promote the growth of the Pinus koraiensis Sieb. et Zucc.

Release of intracellular Zn2+ in cultured neurons after brief exposure to low concentrations of exogenous nitric oxide

BioMetals ◽  
2007 ◽  
Vol 20 (6) ◽  
pp. 891-901 ◽  
Author(s):  
Wei Lin ◽  
Bhavana Mohandas ◽  
Charles P. Fontaine ◽  
Robert A. Colvin
Keyword(s):  
Nitric Oxide ◽  
Cultured Neurons ◽  
Low Concentrations ◽  
Exogenous Nitric Oxide

Detection of Mycobacterium tuberculosis in smear negative sputum by PCR

2012 ◽  
Vol 6 (2) ◽  
pp. 2-6 ◽  
Author(s):  
Mohammad Jobayer ◽  
SM Shamsuzzaman ◽  
Kazi Zulfiquer Mamun
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Pulmonary Tuberculosis ◽  
Accurate Method ◽  
Major Health Problem ◽  
Major Health ◽  
Tuberculosis Patients ◽  
Diagnostic Potential ◽  
Total Death ◽  
Smear Negative ◽  
Negative Sputum

Pulmonary tuberculosis is a major health problem in Bangladesh that is responsible for about 7% of total death in a year. This study was conducted to isolate and identify Mycobacterium tuberculosis from sputum and to evaluate the efficacy of PCR as a modern diagnostic tool, for diagnosis of pulmonary tuberculosis, especially in the smear negative cases. One hundred and fifty suspected pulmonary TB patients (male/ female: 97/53) were included in this study. Single morning sputum was collected from each patient and diagnostic potential of PCR was compared with staining and culture. Twenty five (16.7%) sputum were positive by ZN stained smear. Among 125 smear negative samples, 13 (10.4%) yielded growth in culture in LJ media and 21 (16.8%) samples were positive by PCR. The sensitivity and specificity of PCR in smear negative cases was 100% and 92.9% respectively. Mean detection time in PCR was 24 hours. PCR detected M. tuberculosis in 21 smear negative and 9 culture negative samples. For diagnosis of tuberculosis in smear negative cases, PCR directly from sputum was a very sensitive and accurate method. In conclusion, PCR may be done, especially in clinically suspected pulmonary tuberculosis patients who remain negative by conventional methods.DOI: http://dx.doi.org/10.3329/bjmm.v6i2.19368 Bangladesh J Med Microbiol 2012; 06(02): 2-6

Determination some Immunological Aspects in Pulmonary Tuberculosis Patients in Qadisiyah Province

2018 ◽  
Vol 9 (2) ◽  
Author(s):  
Syoof Khowman Alramahy ◽  
Akram Hadi Hamza
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Pulmonary Tuberculosis ◽  
Statistical Difference ◽  
Positive Culture ◽  
Control Group ◽  
Tuberculosis Patients ◽  
Significant Difference ◽  
Lowenstein Jensen ◽  
The Mean ◽  
Igg Level

This study was carried out to study of some immunological aspects among the pulmonary Tuberculosis patients infected with causative agent, Mycobacterium tuberculosis. A Total of 200 sputum samples were collected from patients attending the consultant Clinic for Chest and Respiratory disease center, Diwaniya. Control group (No=15) also included. According to acid fast stain of sputum, the patients were classified as positive (No=91,45.5%) and negative (No=109,54.5, Lowenstein Jensen medium used for the cultivation of samples, on which 70% of sputum samples where positive culture for this microorganism. The grown microorganism were identified as M. tuberculosis, based on positive A.F.B, Niacin producers ,negative for catlase at 68c. The mean IgG level was l184.053±76.684 mg/100 ml in tuberculosis group compared with 1016.533 ± 44.882 mg/100ml in control group, rendering the statistical difference significant. For IgA and IgM levels, they were at mean of 315.880±38.552 mg/100 ml and 119.527±8.464 mg/100 ml in control group compared with 396.358±38.776 mg/100 ml and 134.207±11.696 mg/100 ml in patients group respectively with significant difference

Cytoplasmic Alkalization Mediates Exogenous Nitric Oxide-Induced Stomatal Closure in Vicia faba

2010 ◽  
Vol 36 (3) ◽  
pp. 533-538 ◽  
Author(s):  
Shi-Ling ZHAO ◽  
Li-Rong SUN ◽  
Huan ZHANG ◽  
Li-Ya MA ◽  
Bao-Shi LU ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Vicia Faba ◽  
Stomatal Closure ◽  
Exogenous Nitric Oxide
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