scholarly journals Enhanced phagocytosis of encapsulated Escherichia coli strains after exposure to sub-MICs of antibiotics is correlated to changes of the bacterial cell surface.

1990 ◽  
Vol 34 (2) ◽  
pp. 332-336 ◽  
Author(s):  
G Raponi ◽  
N Keller ◽  
B P Overbeek ◽  
M Rozenberg-Arska ◽  
K P van Kessel ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Cell Surface ◽  
Bacterial Cell ◽  
Bacterial Cell Surface

Functional Display of an Amoebic Chitinase in Escherichia coli Expressing the Catalytic Domain of EhCHT1 on the Bacterial Cell Surface

2020 ◽  
Vol 192 (4) ◽  
pp. 1255-1269
Author(s):  
Ricardo Torres-Bañaga ◽  
Rosa E. Mares-Alejandre ◽  
Celina Terán-Ramírez ◽  
Ana L. Estrada-González ◽  
Patricia L.A. Muñoz-Muñoz ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Cell Surface ◽  
Bacterial Cell ◽  
Catalytic Domain ◽  
Bacterial Cell Surface

scholarly journals Heterogeneous Surface Expression of EspA Translocon Filaments by Escherichia coli O157:H7 Is Controlled at the Posttranscriptional Level

2003 ◽  
Vol 71 (10) ◽  
pp. 5900-5909 ◽  
Author(s):  
Andrew J. Roe ◽  
Helen Yull ◽  
Stuart W. Naylor ◽  
Martin J. Woodward ◽  
David G. E. Smith ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Cell Surface ◽  
Bacterial Cell ◽  
Bacterial Population ◽  
Enteric Bacteria ◽  
Effector Proteins ◽  
Host Cells ◽  
Phase Variable ◽  
E Coli ◽  
Bacterial Cell Surface

ABSTRACT Type III secretion systems of enteric bacteria enable translocation of effector proteins into host cells. Secreted proteins of verotoxigenic Escherichia coli O157 strains include components of a translocation apparatus, EspA, -B, and -D, as well as “effectors” such as the translocated intimin receptor (Tir) and the mitochondrion-associated protein (Map). This research has investigated the regulation of LEE4 translocon proteins, in particular EspA. EspA filaments could not be detected on the bacterial cell surface when E. coli O157:H7 was cultured in M9 minimal medium but were expressed from only a proportion of the bacterial population when cultured in minimal essential medium modified with 25 mM HEPES. The highest proportions of EspA-filamented bacteria were detected in late exponential phase, after which filaments were lost rapidly from the bacterial cell surface. Our previous research had shown that human and bovine E. coli O157:H7 strains exhibit marked differences in EspD secretion levels. Here it is demonstrated that the proportion of the bacterial population expressing EspA filaments was associated with the level of EspD secretion. The ability of individual bacteria to express EspA filaments was not controlled at the level of LEE1-4 operon transcription, as demonstrated by using both β-galactosidase and green fluorescent protein (GFP) promoter fusions. All bacteria, whether expressing EspA filaments or not, showed equivalent levels of GFP expression when LEE1-4 translational fusions were used. Despite this, the LEE4-espADB mRNA was more abundant from populations with a high proportion of nonsecreting bacteria (low secretors) than from populations with a high proportion of secreting and therefore filamented bacteria (high secretors). This research demonstrates that while specific environmental conditions are required to induce LEE1-4 expression, a further checkpoint exists before EspA filaments are produced on the bacterial surface and secretion of effector proteins occurs. This checkpoint in E. coli O157:H7 translocon expression is controlled by a posttranscriptional mechanism acting on LEE4-espADB mRNA. The heterogeneity in EspA filamentation could arise from phase-variable expression of regulators that control this posttranscriptional mechanism.

scholarly journals Virulence Factors of Uropathogenic Escherichia coli

2021 ◽  
Author(s):  
Etefia Etefia
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract ◽  
Cell Surface ◽  
Virulence Factors ◽  
Bacterial Cell ◽  
Uropathogenic Escherichia Coli ◽  
Bacterial Cell Surface

Uropathogenic Escherichia coli (UPEC) strains are those that cause infections in the urinary tract. They acquired virulence factors which enable them to survive in the urinary tract and elicit pathogenicity. The virulence factors are classified into two categories: (i) bacterial cell surface virulence factors and (ii) bacteria secreted virulence factors. Adhesins, toxins and iron up-take systems are major groups of virulence factors. The variety of virulence factors of UPEC is presented in this chapter.

Fe(iii)-complex mediated bacterial cell surface immobilization of eGFP and enzymes

2021 ◽  
Author(s):  
Lilin Feng ◽  
Liang Gao ◽  
Daniel F. Sauer ◽  
Yu Ji ◽  
Haiyang Cui ◽  
...  
Keyword(s):  
Cell Surface ◽  
Bacterial Cell ◽  
Surface Immobilization ◽  
E Coli ◽  
Bacterial Cell Surface ◽  
Reversible Method

A facile and reversible method to immobilize His6-tagged proteins on the E. coli cell surface through the formation of an Fe(iii)-complex.

scholarly journals Facile method to stain the bacterial cell surface for super-resolution fluorescence microscopy

The Analyst ◽  
2014 ◽  
Vol 139 (12) ◽  
pp. 3174-3178 ◽  
Author(s):  
Ian L. Gunsolus ◽  
Dehong Hu ◽  
Cosmin Mihai ◽  
Samuel E. Lohse ◽  
Chang-soo Lee ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Cell Surface ◽  
Bacterial Cell ◽  
Super Resolution ◽  
Bacterial Cell Surface

The Bacterial Cell Surface in Relation to the Environment

1984 ◽  
pp. 194-219
Author(s):  
Stephen M. Hammond ◽  
Peter A. Lambert ◽  
Andrew N. Rycroft
Keyword(s):  
Cell Surface ◽  
Bacterial Cell ◽  
Bacterial Cell Surface

scholarly journals Cover Feature: Bacterial Cell‐Surface Display of Semisynthetic Cyclic Peptides (ChemBioChem 1/2019)

ChemBioChem ◽  
2018 ◽  
Vol 20 (1) ◽  
pp. 4-4
Author(s):  
Shubhendu Palei ◽  
Kira S. Becher ◽  
Christian Nienberg ◽  
Joachim Jose ◽  
Henning D. Mootz
Keyword(s):  
Cell Surface ◽  
Bacterial Cell ◽  
Cyclic Peptides ◽  
Surface Display ◽  
Cell Surface Display ◽  
Bacterial Cell Surface
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