scholarly journals Detection of a Cephalosporin C Acetyl Esterase in the Carbamate Cephalosporin Antibiotic-Producing Culture, Streptomyces clavuligerus

1972 ◽  
Vol 1 (3) ◽  
pp. 237-241 ◽  
Author(s):  
D. R. Brannon ◽  
D. S. Fukuda ◽  
J. A. Mabe ◽  
F. M. Huber ◽  
J. G. Whitney
Keyword(s):  
Streptomyces Clavuligerus ◽  
Cephalosporin C ◽  
Acetyl Esterase ◽  
Cephalosporin Antibiotic

scholarly journals The conversion of cephalosporins to 7 α-methoxycephalosporins by cell-free extracts of Streptomyces clavuligerus

1980 ◽  
Vol 186 (2) ◽  
pp. 613-616 ◽  
Author(s):  
J O'Sullivan ◽  
E P Abraham
Keyword(s):  
Methoxy Group ◽  
Biosynthetic Pathway ◽  
Streptomyces Clavuligerus ◽  
Ring System ◽  
Reducing Agent ◽  
Cephalosporin C ◽  
Methoxy Derivative

In the presence of S-adenosylmethionine, 2-oxoglutarate, Fe2+ and a reducing agent, cell-free extracts of Streptomyces clavuligerus convert cephalosporin C and O-carbamoyldeacetylcephalosporin C into 7 alpha-methoxy derivatives. No synthesis of a 7 alpha-methoxy derivative of deacetylcephalosporin C was detected in the system used, and the 7 alpha-methoxy derivative of deacetoxycephalosporin C was produced only in relatively small amounts. It appears that the 7 alpha-methoxy group is introduced after the cephalosporin ring system has been formed and that its introduction may represent the final step in a biosynthetic pathway.

A colorimetric assay for the determination of acetyl xylan esterase or cephalosporin C acetyl esterase activities using 7-amino cephalosporanic acid, cephalosporin C, or acetylated xylan as substrate

2007 ◽  
Vol 369 (2) ◽  
pp. 210-217 ◽  
Author(s):  
Irene Martínez-Martínez ◽  
Silvia Montoro-García ◽  
José Daniel Lozada-Ramírez ◽  
Álvaro Sánchez-Ferrer ◽  
Francisco García-Carmona
Keyword(s):  
Colorimetric Assay ◽  
Cephalosporin C ◽  
Acetyl Xylan Esterase ◽  
Acetyl Esterase ◽  
Esterase Activities

scholarly journals Purification and characterization of cephalosporin 7α-hydroxylase from Streptomyces clavuligerus

1991 ◽  
Vol 280 (2) ◽  
pp. 471-474 ◽  
Author(s):  
X Xiao ◽  
S Wolfe ◽  
A L Demain
Keyword(s):  
Gel Filtration ◽  
Streptomyces Clavuligerus ◽  
Exchange Chromatography ◽  
Cephalosporin C ◽  
Hydroxylase Activity ◽  
Purification And Characterization ◽  
Major Band ◽  
Sds Page ◽  
Optimum Ph

Cephalosporin 7 alpha-hydroxylase, which catalyses the conversion of cephalosporins into their 7 alpha-hydroxy derivatives, was purified nearly 390-fold from Streptomyces clavuligerus through ion-exchange chromatography, (NH4)2SO4 fractionation, gel filtration and dye chromatography, with the use of h.p.l.c. to monitor enzyme activity. The nearly pure enzyme migrates as a single major band, with an Mr of 32,000 in SDS/PAGE. Its optimum pH is in the range 7.3-7.7. Under our conditions the reaction was fastest at temperatures in the range 20-30 degrees C. The Km for cephalosporin C is 0.72 mM, and the Vmax. is 15.4 mumol of cephalosporin C hydroxylated/min per mg. Cephalosporin 7 alpha-hydroxylase did not show any deacetoxycephalosporin C synthase or deacetoxycephalosporin C hydroxylase activity.

scholarly journals Specialized Metabolites from Ribosome Engineered Strains of Streptomyces clavuligerus

Metabolites ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 239
Author(s):  
Arshad Ali Shaikh ◽  
Louis-Felix Nothias ◽  
Santosh K. Srivastava ◽  
Pieter C. Dorrestein ◽  
Kapil Tahlan
Keyword(s):  
In Silico Analysis ◽  
Cost Effective ◽  
Gene Clusters ◽  
Streptomyces Clavuligerus ◽  
Strain Engineering ◽  
Ribosome Recycling Factor ◽  
Metabolic Potential ◽  
Putative Gene ◽  
Specialized Metabolites ◽  
Streptomyces Spp

Bacterial specialized metabolites are of immense importance because of their medicinal, industrial, and agricultural applications. Streptomyces clavuligerus is a known producer of such compounds; however, much of its metabolic potential remains unknown, as many associated biosynthetic gene clusters are silent or expressed at low levels. The overexpression of ribosome recycling factor (frr) and ribosome engineering (induced rpsL mutations) in other Streptomyces spp. has been reported to increase the production of known specialized metabolites. Therefore, we used an overexpression strategy in combination with untargeted metabolomics, molecular networking, and in silico analysis to annotate 28 metabolites in the current study, which have not been reported previously in S. clavuligerus. Many of the newly described metabolites are commonly found in plants, further alluding to the ability of S. clavuligerus to produce such compounds under specific conditions. In addition, the manipulation of frr and rpsL led to different metabolite production profiles in most cases. Known and putative gene clusters associated with the production of the observed compounds are also discussed. This work suggests that the combination of traditional strain engineering and recently developed metabolomics technologies together can provide rapid and cost-effective strategies to further speed up the discovery of novel natural products.

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