scholarly journals A Multispecies Cluster of VIM-1 Carbapenemase-Producing Enterobacterales Linked by a Novel, Highly Conjugative, and Broad-Host-Range IncA Plasmid Forebodes the Reemergence of VIM-1

2020 ◽  
Vol 64 (4) ◽  
Author(s):  
Gabriele Arcari ◽  
Federica Maria Di Lella ◽  
Giulia Bibbolino ◽  
Fabio Mengoni ◽  
Marzia Beccaccioli ◽  
...  
Keyword(s):  
Host Range ◽  
Bacterial Species ◽  
Klebsiella Oxytoca ◽  
Gene Cassette ◽  
University Hospital ◽  
Broad Host Range ◽  
Class 1 Integron ◽  
Content Type ◽  
Carbapenem Resistant ◽  
Class 1

ABSTRACT In this study, we investigated VIM-1-producing Escherichia coli, Klebsiella oxytoca, Klebsiella pneumoniae, Citrobacter freundii, and Enterobacter cloacae strains, isolated in 2019 during a period of active surveillance of carbapenem-resistant Enterobacterales in a large university hospital in Italy. VIM-1-producing strains colonized the gut of patients, with up to three different VIM-1-positive bacterial species isolated from a single rectal swab, but also caused bloodstream infection in one colonized patient. In the multispecies cluster, blaVIM-1 was identified in a 5-gene cassette class 1 integron, associated with several genetic determinants, including the blaSHV-12, qnrS1, and mph(A) genes, located on a highly conjugative and broad-host-range IncA plasmid. The characteristics and origin of this IncA plasmid were studied.

scholarly journals Clinical Carbapenem-Resistant Acinetobacter baylyi Strain CoharboringblaSIM-1andblaOXA-23from China

2011 ◽  
Vol 55 (11) ◽  
pp. 5347-5349 ◽  
Author(s):  
Zhihui Zhou ◽  
Xiaoxing Du ◽  
Li Wang ◽  
Qing Yang ◽  
Yiqi Fu ◽  
...  
Keyword(s):  
Genetic Structure ◽  
Acinetobacter Baumannii ◽  
Clonal Complex ◽  
Gene Cassette ◽  
Large Plasmid ◽  
Acinetobacter Baylyi ◽  
Class 1 Integron ◽  
Content Type ◽  
Carbapenem Resistant ◽  
Class 1

ABSTRACTblaSIM-1andblaOXA-23were codetected in clinical carbapenem-resistantAcinetobacter baylyistrain NB09A30. Both of carbapenemase genes were located on a large plasmid (ca. 360 kb).blaSIM-1was found as a gene cassette inserted into a class 1 integron identical to that determined inAcinetobactersp. isolates from South Korea. The genetic structure ofblaOXA-23in NB09A30 was different from that in the prevalentAcinetobacter baumanniiof clonal complex 92 (CC92) from the same hospital.

scholarly journals Metallo-β-Lactamase Gene blaIMP-15 in a Class 1 Integron, In95, from Pseudomonas aeruginosa Clinical Isolates from a Hospital in Mexico

2008 ◽  
Vol 52 (8) ◽  
pp. 2943-2946 ◽  
Author(s):  
U. Garza-Ramos ◽  
R. Morfin-Otero ◽  
H. S. Sader ◽  
R. N. Jones ◽  
E. Hernández ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Tertiary Care Hospital ◽  
Tertiary Care ◽  
Gene Cassette ◽  
Clinical Isolates ◽  
Care Hospital ◽  
Class 1 Integron ◽  
Carbapenem Resistant ◽  
Class 1 ◽  
Lactamase Gene

ABSTRACT During 2003, 40 carbapenem-resistant Pseudomonas aeruginosa clinical isolates collected in a Mexican tertiary-care hospital were screened for metallo-β-lactamase production. Thirteen isolates produced IMP-15, and 12 had a single pulsed-field gel electrophoresis pattern. The bla IMP-15 gene cassette was inserted in a plasmid-borne integron with a unique array of gene cassettes and was named In95.

scholarly journals Infrequent Finding of Metallo-β-Lactamase VIM-2 in Carbapenem-Resistant Pseudomonas aeruginosa Strains from Croatia

2012 ◽  
Vol 56 (5) ◽  
pp. 2746-2749 ◽  
Author(s):  
Sanda Sardelic ◽  
Branka Bedenic ◽  
Céline Colinon-Dupuich ◽  
Stjepan Orhanovic ◽  
Zrinka Bosnjak ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Coastal Region ◽  
Large Hospital ◽  
Class 1 Integron ◽  
Content Type ◽  
Variable Part ◽  
Carbapenem Resistant ◽  
Class 1 ◽  
Sequence Types

ABSTRACTOne hundred sixty-nine nonreplicate imipenem-resistantPseudomonas aeruginosastrains isolated in a large hospital on the coastal region of Croatia were studied. The most active antibiotics were colistin and amikacin. Most of the isolates were multiresistant. The most prevalent serotype was O12, followed by O11. Six strains carried theblaVIM-2gene located in a novel class 1 integron composed in its variable part of theblaVIM-2-blaoxa-10-ΔqacF-aacA4genes. Metallo-β-lactamase-producing strains belonged to sequence types ST235 and ST111.

scholarly journals First Report ofblaIMP-14on a Plasmid Harboring Multiple Drug Resistance Genes in Escherichia coli Sequence Type 131

2016 ◽  
Vol 60 (8) ◽  
pp. 5068-5071 ◽  
Author(s):  
Nicole Stoesser ◽  
Anna E. Sheppard ◽  
Gisele Peirano ◽  
Robert P. Sebra ◽  
Tarah Lynch ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Multiple Drug Resistance ◽  
Carbapenem Resistance ◽  
Sequence Type ◽  
Multiple Drug ◽  
Class 1 Integron ◽  
Content Type ◽  
Carbapenem Resistant ◽  
Class 1

ABSTRACTTheblaIMP-14carbapenem resistance gene has largely previously been observed inPseudomonas aeruginosaandAcinetobacterspp. As part of global surveillance and sequencing of carbapenem-resistantEscherichia coli, we identified a sequence type 131 strain harboringblaIMP-14within a class 1 integron, itself nested within an ∼54-kb multidrug resistance region on an epidemic IncA/C2plasmid. The emergence ofblaIMP-14in this context in the ST131 lineage is of potential clinical concern.

scholarly journals Genetic Modification of Sodalis Species by DNA Transduction

mSphere ◽  
2021 ◽  
Vol 6 (1) ◽  
Author(s):  
Chelsea M. Keller ◽  
Christopher G. Kendra ◽  
Roberto E. Bruna ◽  
David Craft ◽  
Mauricio H. Pontes
Keyword(s):  
Host Range ◽  
Genetic Study ◽  
Genetic Manipulation ◽  
Bacterial Species ◽  
Broad Host Range ◽  
Insect Host ◽  
Exogenous Dna ◽  
Content Type ◽  
Sodalis Glossinidius ◽  
Genetically Manipulated

ABSTRACT Bacteriophages (phages) are ubiquitous in nature. These viruses play a number of central roles in microbial ecology and evolution by, for instance, promoting horizontal gene transfer (HGT) among bacterial species. The ability of phages to mediate HGT through transduction has been widely exploited as an experimental tool for the genetic study of bacteria. As such, bacteriophage P1 represents a prototypical generalized transducing phage with a broad host range that has been extensively employed in the genetic manipulation of Escherichia coli and a number of other model bacterial species. Here we demonstrate that P1 is capable of infecting, lysogenizing, and promoting transduction in members of the bacterial genus Sodalis, including the maternally inherited insect endosymbiont Sodalis glossinidius. While establishing new tools for the genetic study of these bacterial species, our results suggest that P1 may be used to deliver DNA to many Gram-negative endosymbionts in their insect host, thereby circumventing a culturing requirement to genetically manipulate these organisms. IMPORTANCE A large number of economically important insects maintain intimate associations with maternally inherited endosymbiotic bacteria. Due to the inherent nature of these associations, insect endosymbionts cannot be usually isolated in pure culture or genetically manipulated. Here we use a broad-host-range bacteriophage to deliver exogenous DNA to an insect endosymbiont and a closely related free-living species. Our results suggest that broad-host-range bacteriophages can be used to genetically alter insect endosymbionts in their insect host and, as a result, bypass a culturing requirement to genetically alter these bacteria.

Occurrence of intI1-associated VIM-5 carbapenemase and co-existence of all four classes of β-lactamase in carbapenem-resistant clinical Pseudomonas aeruginosa DMC-27b

2019 ◽  
Author(s):  
M Ishrat Jahan ◽  
M Mizanur Rahaman ◽  
M Anwar Hossain ◽  
Munawar Sultana
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Gene Cassette ◽  
Limiting Current ◽  
Class 1 Integron ◽  
Active Efflux ◽  
Carbapenem Resistant ◽  
Porin Protein ◽  
Class 1 ◽  
Rapid Dissemination ◽  
Resistance Traits

Abstract Objectives Emergence of carbapenem-resistant Pseudomonas aeruginosa is limiting current treatment options. Carbapenemases and their association with integrons can cause rapid dissemination of resistance traits. We report here the co-existence and chromosomal inheritance of all four classes of β-lactamase and the presence of a unique class 1 integron (intI1) harbouring blaVIM-5 within a single isolate of P. aeruginosa, DMC-27b. Methods DMC-27b, isolated from urine, was characterized for carbapenem resistance both phenotypically and genotypically. The orientation of gene cassette structures of class 1 integrons was determined using referenced and designed overlapping primers and complete genome sequence (CGS) data. The antimicrobial resistance profile, porin protein mutations and the presence of active efflux activity were studied from the CGS. Results P. aeruginosa DMC-27b was resistant to a total of 20 antibiotics, with imipenem and meropenem MIC90s of >512 mg/L. The isolate harboured all four classes of β-lactamase: VEB-1 (class A), VIM-5 (class B), PDC-35 (class C) and OXA-2 and OXA-50 (both class D). Chromosomal harbouring of blaVIM-5 was associated with the intI1 gene cassette as the sole gene, a unique cassette so far reported. A total of 11 mutations, among them some mutations causing extra folds and changes in binding sites, in porin protein OprD might also affect its functionality regarding the transportation of antibiotics. Conclusions This is one of the earliest reports of its kind on the co-existence of all four β-lactamase classes in P. aeruginosa DMC-27b. Acquisition of multiple resistance determinants is paving the way for the development of MDR. This superbug is a model for rapid dissemination of resistance traits both horizontally and vertically.

scholarly journals First Description of KPC-2-Producing Klebsiella oxytoca in Brazil

2013 ◽  
Vol 57 (8) ◽  
pp. 4077-4078 ◽  
Author(s):  
Anna C. S. Almeida ◽  
Felipe L. S. Cavalcanti ◽  
Willames M. B. Martins ◽  
Marinalda A. Vilela ◽  
Ana C. Gales ◽  
...  
Keyword(s):  
Klebsiella Oxytoca ◽  
High Variability ◽  
Resistance Determinant ◽  
Class 1 Integron ◽  
Content Type ◽  
Class A ◽  
First Case ◽  
Class 1

ABSTRACTThe present work reports the detection of the first case of nosocomialKlebsiella oxytocaproducing class A carbapenemase KPC-2 in Brazil. The isolate KPN106 carried a 65-kb IncW-type plasmid that harbors theblaKPCgene and Tn4401b. Moreover, we detected the presence of a class 1 integron containing a new allele,arr-8, followed by a 5′-truncateddhfrIIIcgene. In view of the recent results, we emphasize the high variability of the bacterial and genetic hosts of this resistance determinant.

scholarly journals OXA-198, an Acquired Carbapenem-Hydrolyzing Class D β-Lactamase from Pseudomonas aeruginosa

2011 ◽  
Vol 55 (10) ◽  
pp. 4828-4833 ◽  
Author(s):  
Farid El Garch ◽  
Pierre Bogaerts ◽  
Carine Bebrone ◽  
Moreno Galleni ◽  
Youri Glupczynski
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Carbapenem Resistance ◽  
Amino Acid Identity ◽  
Ventilator Associated Pneumonia ◽  
Class 1 Integron ◽  
Content Type ◽  
New Class ◽  
Carbapenem Resistant ◽  
Class D ◽  
Class 1

ABSTRACTA carbapenem-resistantPseudomonas aeruginosastrain (PA41437) susceptible to expanded-spectrum cephalosporins was recovered from several consecutive lower-respiratory-tract specimens of a patient who developed a ventilator-associated pneumonia while hospitalized in an intensive care unit. Cloning experiments identified OXA-198, a new class D β-lactamase which was weakly related (less than 45% amino acid identity) to other class D β-lactamases. Expression inEscherichia coliTOP10 and inP. aeruginosaPAO1 led to transformants that were resistant to ticarcillin and showed reduced susceptibility to carbapenems and cefepime. TheblaOXA-198gene was harbored by a class 1 integron carried by a ca. 46-kb nontypeable plasmid. This study describes a novel class D β-lactamase involved in carbapenem resistance inP. aeruginosa.

scholarly journals SMB-1, a Novel Subclass B3 Metallo-β-Lactamase, Associated with ISCR1and a Class 1 Integron, from a Carbapenem-Resistant Serratia marcescens Clinical Isolate

2011 ◽  
Vol 55 (11) ◽  
pp. 5143-5149 ◽  
Author(s):  
Jun-ichi Wachino ◽  
Hiroyuki Yoshida ◽  
Kunikazu Yamane ◽  
Satowa Suzuki ◽  
Mari Matsui ◽  
...  
Keyword(s):  
Serratia Marcescens ◽  
Clinical Isolate ◽  
Apple Orchard ◽  
Resistance Pattern ◽  
Binding Motif ◽  
Class 1 Integron ◽  
Uncultured Bacterium ◽  
Content Type ◽  
Carbapenem Resistant ◽  
Class 1

ABSTRACTA carbapenem-resistantSerratia marcescensstrain, 10mdr148, was identified in a Japanese hospital in 2010. The carbapenem resistance of this strain was attributed to the production of a novel metallo-β-lactamase (MBL), named SMB-1 (Serratiametallo-β-lactamase). SMB-1 possessed a zinc binding motif, H(Q)XHXDH (residues 116 to 121), H196, and H263 and was categorized as a member of subclass B3 MBL. SMB-1 has 75% amino acid identity with the most closely related MBL, AMO1, of uncultured bacterium, recently identified through the metagenomic analysis of apple orchard soil. The introduction ofblaSMB-1intoEscherichia coliconferred resistance to a variety of β-lactam antibiotics, penicillins, cephalosporins, and carbapenems, but not aztreonam, a resistance pattern consistent with those of other MBLs. SMB-1 demonstrated highkcatvalues of >500 s−1for carbapenems, resulting in the highest hydrolyzing efficiency (kcat/Km) among the agents tested. The hydrolyzing activity of SMB-1 was well inhibited by chelating agents. TheblaSMB-1gene was located on the chromosome ofS. marcescensstrain 10mdr148 and at the 3′ end of the ISCR1element in complex with a typical class 1 integron carryingaac(6′)-IbandcatB3gene cassettes. Downstream ofblaSMB-1, the second copy of the 3′conserved segment and ISCR1were found. To our knowledge, this is the first subclass B3 MBL gene associated with an ISCR1element identified in anEnterobacteriaceaeclinical isolate. A variety of antibiotic resistance genes embedded with ISCR1have been widely spread amongEnterobacteriaceaeclinical isolates, thus the further dissemination ofblaSMB-1mediated by ISCR1transposition activity may become a future concern.

scholarly journals Coproduction of KPC-2 and IMP-10 in Carbapenem-Resistant Serratia marcescens Isolates from an Outbreak in a Brazilian Teaching Hospital

2015 ◽  
Vol 53 (7) ◽  
pp. 2324-2328 ◽  
Author(s):  
Kesia Esther Silva ◽  
Rodrigo Cayô ◽  
Cecilia Godoy Carvalhaes ◽  
Flávia Patussi Correia Sacchi ◽  
Fernanda Rodrigues-Costa ◽  
...  
Keyword(s):  
Antimicrobial Agent ◽  
Serratia Marcescens ◽  
Teaching Hospital ◽  
Class 1 Integron ◽  
Content Type ◽  
New Class ◽  
Carbapenem Resistant ◽  
Class 1

We describe an outbreak caused by KPC-2- and IMP-10-producingSerratia marcescensisolates in a Brazilian teaching hospital. Tigecycline was the only active antimicrobial agent tested. TheblaIMP-10gene was located in a new class 1 integron, namedIn990, carried by a nonconjugative plasmid, in contrast toblaKPC-2.

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