scholarly journals The forebrain synaptic transcriptome is organized by clocks but its proteome is driven by sleep

Science ◽  
2019 ◽  
Vol 366 (6462) ◽  
pp. eaav2642 ◽  
Author(s):  
Sara B. Noya ◽  
David Colameo ◽  
Franziska Brüning ◽  
Andrea Spinnler ◽  
Dennis Mircsof ◽  
...  
Keyword(s):  
Sleep Deprivation ◽  
Messenger Rna ◽  
Energy Homeostasis ◽  
Time Of Day ◽  
Daily Rhythms ◽  
Transcript Accumulation ◽  
Biochemical Approach ◽  
Functional Relevance ◽  
Axonal Arbors

Neurons have adapted mechanisms to traffic RNA and protein into distant dendritic and axonal arbors. Taking a biochemical approach, we reveal that forebrain synaptic transcript accumulation shows overwhelmingly daily rhythms, with two-thirds of synaptic transcripts showing time-of-day–dependent abundance independent of oscillations in the soma. These transcripts formed two sharp temporal and functional clusters, with transcripts preceding dawn related to metabolism and translation and those anticipating dusk related to synaptic transmission. Characterization of the synaptic proteome around the clock demonstrates the functional relevance of temporal gating for synaptic processes and energy homeostasis. Unexpectedly, sleep deprivation completely abolished proteome but not transcript oscillations. Altogether, the emerging picture is one of a circadian anticipation of messenger RNA needs in the synapse followed by translation as demanded by sleep-wake cycles.

scholarly journals Transcript Profiling in the Barley Mildew Pathogen Blumeria graminis by Serial Analysis of Gene Expression (SAGE)

2002 ◽  
Vol 15 (8) ◽  
pp. 847-856 ◽  
Author(s):  
Stephen W. Thomas ◽  
Mikkel A. Glaring ◽  
Søren W. Rasmussen ◽  
Julia T. Kinane ◽  
Richard P. Oliver
Keyword(s):  
Gene Expression ◽  
Fungal Pathogen ◽  
Messenger Rna ◽  
Germ Tube ◽  
Blumeria Graminis ◽  
Appressorium Formation ◽  
Transcript Accumulation ◽  
Host Cell Wall ◽  
Barley Leaf

The fungal pathogen Blumeria graminis f. sp. hordei develops on the barley leaf via distinct, morphologically well-defined stages. After landing on a host plant, the conidia rapidly germinate to form a primary germ tube. Subsequently, an appressorial germ tube emerges from the conidium and differentiates an appressorium from which penetration of the host cell wall is attempted. We have used serial analysis of gene expression to provide a measurement of messenger RNA contents in ungerminated conidia, during conidial germination, and during appressorium formation. The resulting data provide a resource for the characterization of changes in transcript accumulation during early development of B. graminis.

scholarly journals Mutator-Suppressible Alleles of rough sheath1 and liguleless3 in Maize Reveal Multiple Mechanisms for Suppression

Genetics ◽  
2000 ◽  
Vol 154 (1) ◽  
pp. 437-446 ◽  
Author(s):  
Lisa Girard ◽  
Michael Freeling
Keyword(s):  
Untranslated Region ◽  
Mutant Allele ◽  
Negative Regulation ◽  
Wild Type ◽  
Knox Gene ◽  
Transcript Accumulation ◽  
Mu Suppression ◽  
Different Types ◽  
Rna Blot Analysis

Abstract Insertions of Mutator transposons into maize genes can generate suppressible alleles. Mu suppression is when, in the absence of Mu activity, the phenotype of a mutant allele reverts to that of its progenitor. Here we present the characterization of five dominant Mu-suppressible alleles of the knox (knotted1-like homeobox) genes liguleless3 and rough sheath1, which exhibit neomorphic phenotypes in the leaves. RNA blot analysis suggests that Mu suppression affects only the neomorphic aspect of the allele, not the wild-type aspect. Additionally, Mu suppression appears to be exerting its effects at the level of transcription or transcript accumulation. We show that truncated transcripts are produced by three alleles, implying a mechanism for Mu suppression of 5′ untranslated region insertion alleles distinct from that which has been described previously. Additionally, it is found that Mu suppression can be caused by at least three different types of Mutator elements. Evidence presented here suggests that whether an allele is suppressible or not may depend upon the site of insertion. We cite previous work on the knox gene kn1, and discuss our results in the context of interactions between Mu-encoded products and the inherently negative regulation of neomorphic liguleless3 and rough sheath1 transcription.

Assessing vigilance through a brief pencil and paper letter cancellation task (LCT): effects of one night of sleep deprivation and of the time of day

Ergonomics ◽  
1997 ◽  
Vol 40 (6) ◽  
pp. 613-630 ◽  
Author(s):  
MARIA CASAGRANDE ◽  
CRISTIANO VIOLANI ◽  
GIUSEPPE CURCIO ◽  
MARIO BERTINI
Keyword(s):  
Sleep Deprivation ◽  
Time Of Day ◽  
Cancellation Task ◽  
Pencil And Paper

Characterization of striatal neurons expressing high levels of glutamic acid decar☐ylase messenger RNA

1989 ◽  
Vol 492 (1-2) ◽  
pp. 237-244 ◽  
Author(s):  
Marie-Francoise Chesselet ◽  
Elaine Robbins
Keyword(s):  
Glutamic Acid ◽  
Messenger Rna ◽  
Striatal Neurons
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