scholarly journals Single-cell profiling of the developing mouse brain and spinal cord with split-pool barcoding

Science ◽  
2018 ◽  
Vol 360 (6385) ◽  
pp. 176-182 ◽  
Author(s):  
Alexander B. Rosenberg ◽  
Charles M. Roco ◽  
Richard A. Muscat ◽  
Anna Kuchina ◽  
Paul Sample ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Single Cell ◽  
Mouse Brain ◽  
Single Cell Profiling ◽  
Developing Mouse Brain ◽  
Brain And Spinal Cord

Assessment of Ultrastructural Neuroplasticity Parameters After In Utero Transduction of the Developing Mouse Brain and Spinal Cord

10.3791/59084 ◽  
2019 ◽  
Author(s):  
David Lutz ◽  
Monika von Düring ◽  
Franco Corvace ◽  
Luzie Augustinowski ◽  
Anne-Kathrin Trampe ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Mouse Brain ◽  
In Utero ◽  
Developing Mouse Brain ◽  
Brain And Spinal Cord

scholarly journals Single-cell Sequencing Reveals Brain/Spinal Cord Oligodendrocyte Precursor Heterogeneity and Requirement for mTOR in Cholesterol Biosynthesis and Myelin Maintenance

2020 ◽  
Author(s):  
Luipa Khandker ◽  
Marisa A. Jeffries ◽  
Yun-Juan Chang ◽  
Marie L. Mather ◽  
Jennifer N. Bourne ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Single Cell ◽  
Single Cell Analysis ◽  
Cholesterol Biosynthesis ◽  
Adult Brain ◽  
Cell Analysis ◽  
Sequencing Studies ◽  
Oligodendrocyte Precursor ◽  
Brain And Spinal Cord ◽  
The Brain

AbstractBrain and spinal cord oligodendroglia have distinct functional characteristics, and cell autonomous loss of individual genes can result in different regional phenotypes. However, sequencing studies to date have not revealed distinctions between brain and spinal cord oligodendroglia. Using single-cell analysis of oligodendroglia during myelination, we demonstrate that brain and spinal cord precursors are transcriptionally distinct, defined predominantly by cholesterol biosynthesis. We further identify mechanistic target of rapamycin (mTOR) as a major regulator promoting cholesterol biosynthesis in oligodendroglia. Oligodendroglial-specific loss of mTOR compromises cholesterol biosynthesis in both the brain and spinal cord. Importantly, mTOR loss has a greater impact on cholesterol biosynthesis in spinal cord oligodendroglia that corresponds with more pronounced developmental deficits. However, loss of mTOR in brain oligodendroglia ultimately results in oligodendrocyte death, spontaneous demyelination, and impaired axonal function, demonstrating that mTOR is required for myelin maintenance in the adult brain.

Modulation of [3H]DAGO binding by substance P (SP) and SP fragments in the mouse brain and spinal cord via MU1 interactions

Neuropeptides ◽  
1989 ◽  
Vol 13 (4) ◽  
pp. 225-233 ◽  
Author(s):  
S.A. Krumins ◽  
D.C. Kim ◽  
V.S. Seybold ◽  
A.A. Larson
Keyword(s):  
Spinal Cord ◽  
Substance P ◽  
Mouse Brain ◽  
Brain And Spinal Cord
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