Erratum for the Report “ESCRT III repairs nuclear envelope ruptures during cell migration to limit DNA damage and cell death” by M. Raab, M. Gentili, H. de Belly, H. R. Thiam, P. Vargas, A. J. Jimenez, F. Lautenschlaeger, Raphaël Voituriez, A. M. Lennon-Duménil, N. Manel, M. Piel

Science ◽  
2016 ◽  
Vol 353 (6298) ◽  
pp. aah6167
Keyword(s):  
Dna Damage ◽  
Cell Death ◽  
Cell Migration ◽  
Nuclear Envelope

ESCRT III repairs nuclear envelope ruptures during cell migration to limit DNA damage and cell death

Science ◽  
2016 ◽  
Vol 352 (6283) ◽  
pp. 359-362 ◽  
Author(s):  
M. Raab ◽  
M. Gentili ◽  
H. de Belly ◽  
H.-R. Thiam ◽  
P. Vargas ◽  
...  
Keyword(s):  
Dna Damage ◽  
Cell Death ◽  
Cell Migration ◽  
Nuclear Envelope

scholarly journals Actin dynamics regulation by TTC7A/PI4KIIIα axis limits DNA damage and cell death during leukocyte migration

2021 ◽  
Author(s):  
Tania Gajardo ◽  
Marie Lo ◽  
Mathilde Bernard ◽  
Claire Leveau ◽  
Marie-Therese El-Daher ◽  
...  
Keyword(s):  
Dna Damage ◽  
Cell Death ◽  
Cell Migration ◽  
Actin Cytoskeleton ◽  
Leukocyte Migration ◽  
Actin Dynamics ◽  
Immune Homeostasis ◽  
Cellular Functions ◽  
The Impact

The actin cytoskeleton has a crucial role in the maintenance of the immune homeostasis by controlling various cell processes, including cell migration. Mutations in the TTC7A gene have been described as the cause of a primary immunodeficiency associated to different degrees of gut involvement and alterations in the actin cytoskeleton dynamics. Although several cellular functions have been associated with TTC7A, the role of the protein in the maintenance of the immune homeostasis is still poorly understood. Here we leverage microfabricated devices to investigate the impact of TTC7A deficiency in leukocytes migration at the single cell level. We show that TTC7A-deficient leukocytes exhibit an altered cell migration and reduced capacity to deform through narrow gaps. Mechanistically, TTC7A-deficient phenotype resulted from impaired phosphoinositides signaling, leading to the downregulation of the PI3K/AKT/RHOA regulatory axis and imbalanced actin cytoskeleton dynamic. This resulted in impaired cell motility, accumulation of DNA damage and increased cell death during chemotaxis in dense 3D gels. Our results highlight a novel role of TTC7A as a critical regulator of leukocyte migration. Impairment of this cellular function is likely to contribute to pathophysiology underlying progressive immunodeficiency in patients.

Oxidative Stress, Ocular Disease and Diabetes Retinopathy

2019 ◽  
Vol 24 (40) ◽  
pp. 4726-4741 ◽  
Author(s):  
Orathai Tangvarasittichai ◽  
Surapon Tangvarasittichai
Keyword(s):  
Oxidative Stress ◽  
Dna Damage ◽  
Cell Death ◽  
Protein Modification ◽  
Morphological Changes ◽  
Corneal Dystrophy ◽  
Age Related Macular Degeneration ◽  
Ocular Diseases ◽  
Retinal Light Damage ◽  
Age Related

Background: Oxidative stress is caused by free radicals or oxidant productions, including lipid peroxidation, protein modification, DNA damage and apoptosis or cell death and results in cellular degeneration and neurodegeneration from damage to macromolecules. Results: Accumulation of the DNA damage (8HOdG) products and the end products of LPO (including aldehyde, diene, triene conjugates and Schiff’s bases) were noted in the research studies. Significantly higher levels of these products in comparison with the controls were observed. Oxidative stress induced changes to ocular cells and tissues. Typical changes include ECM accumulation, cell dysfunction, cell death, advanced senescence, disarrangement or rearrangement of the cytoskeleton and released inflammatory cytokines. It is involved in ocular diseases, including keratoconus, Fuchs endothelial corneal dystrophy, and granular corneal dystrophy type 2, cataract, age-related macular degeneration, primary open-angle glaucoma, retinal light damage, and retinopathy of prematurity. These ocular diseases are the cause of irreversible blindness worldwide. Conclusions: Oxidative stress, inflammation and autophagy are implicated in biochemical and morphological changes in these ocular tissues. The development of therapy is a major target for the management care of these ocular diseases.

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