scholarly journals In vivo gene editing in dystrophic mouse muscle and muscle stem cells

Science ◽  
2015 ◽  
Vol 351 (6271) ◽  
pp. 407-411 ◽  
Author(s):  
M. Tabebordbar ◽  
K. Zhu ◽  
J. K. W. Cheng ◽  
W. L. Chew ◽  
J. J. Widrick ◽  
...  
Keyword(s):  
Stem Cells ◽  
Gene Editing ◽  
Mouse Muscle ◽  
Muscle Stem Cells ◽  
Dystrophic Mouse

scholarly journals Differential cell fates of muscle stem cells are accompanied by symmetric segregation of canonical H3 histones in vivo

2018 ◽  
Author(s):  
Brendan Evano ◽  
Gilles Le Carrou ◽  
Geneviève Almouzni ◽  
Shahragim Tajbakhsh
Keyword(s):  
Stem Cells ◽  
Ex Vivo ◽  
Germline Stem Cells ◽  
Cell Fates ◽  
Evolutionary Mechanisms ◽  
Mouse Muscle ◽  
Muscle Stem Cells ◽  
Histone H3 Variant ◽  
Cell Assays

AbstractStem cells are maintained through symmetric or asymmetric cell divisions. While various mechanisms initiate asymmetric cell fates during mitosis, possible epigenetic control of this process has emerged recently. The asymmetrical distribution of a canonical histone H3 variant during mitosis in fly germline has suggested a role for partitioning old and new nucleosomes in asymmetric cell fates. Here, we provide resources for single cell assays and show the asymmetric segregation of transcription factors along with old and new DNA in mouse muscle stem cells ex vivo and in vivo. However, these differential fate outcomes contrast with a symmetric distribution of the canonical H3.1 vertebrate variant. These findings point to different evolutionary mechanisms operating in fly germline stem cells and vertebrate somatic stem cells to mitigate epigenetic regulation of asymmetric cell fates.

scholarly journals 483. In Vivo DMD Gene Editing in Muscles and Muscle Stem Cells of Dystrophic Mice

2016 ◽  
Vol 24 ◽  
pp. S191-S192 ◽  
Author(s):  
Mohammadsharif Tabebordbar ◽  
Kexian Zhu ◽  
Jason Cheng ◽  
Jeffrey Widrick ◽  
Winston Yan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Gene Editing ◽  
Muscle Stem Cells ◽  
Dmd Gene ◽  
Dystrophic Mice

scholarly journals In Vivo Gene Editing of Muscle Stem Cells with Adeno-Associated Viral Vectors in a Mouse Model of Duchenne Muscular Dystrophy

2020 ◽  
Vol 19 ◽  
pp. 320-329
Author(s):  
Jennifer B. Kwon ◽  
Adarsh R. Ettyreddy ◽  
Ashish Vankara ◽  
Joel D. Bohning ◽  
Garth Devlin ◽  
...  
Keyword(s):  
Stem Cells ◽  
Duchenne Muscular Dystrophy ◽  
Muscular Dystrophy ◽  
Mouse Model ◽  
Viral Vectors ◽  
Gene Editing ◽  
Muscle Stem Cells

Analysis of the hematopoietic potential of muscle-derived cells in mice

Blood ◽  
2002 ◽  
Vol 100 (2) ◽  
pp. 721-723 ◽  
Author(s):  
Hartmut Geiger ◽  
Jarrod M. True ◽  
Barry Grimes ◽  
Elizabeth J. Carroll ◽  
Roger A. Fleischman ◽  
...  
Keyword(s):  
Stem Cells ◽  
Muscle Tissue ◽  
Cell Sorting ◽  
Hematopoietic Stem ◽  
Hematopoietic System ◽  
Muscle Stem Cells ◽  
Antibody Staining ◽  
Stem And Progenitor Cells

Abstract Cells in murine muscle have been reported to differentiate into hematopoietic stem and progenitor cells and thus repopulate the hematopoietic system of an irradiated animal. This activity was attributed to muscle stem cells. We used an in vitro and in vivo approach to identify the hematopoietic repopulating activity found in muscle tissue of mice by antibody staining and cell sorting. We confirmed existence of a hematopoietic repopulating cell in muscle tissue, but the data strongly suggest that repopulation is due not to muscle stem cells but to hematopoietic cells present in muscle tissue. Unexpectedly, the blood-forming cells were enriched in muscle relative to their frequency in peripheral blood.

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