Stimulation by immune complexes of mucus release from goblet cells of the rat small intestine

Science ◽  
1977 ◽  
Vol 197 (4301) ◽  
pp. 370-372 ◽  
Author(s):  
W. Walker ◽  
M Wu ◽  
K. Bloch
Keyword(s):  
Small Intestine ◽  
Immune Complexes ◽  
Goblet Cells ◽  
Rat Small Intestine

scholarly journals Quantitative Changes in the Goblet Cells of the Rat Small Intestine after Irradiation

1985 ◽  
Vol 24 (3) ◽  
pp. 291-299 ◽  
Author(s):  
A. Becciolini ◽  
D. Fabbrica ◽  
D. Cremonini ◽  
M. Balzi
Keyword(s):  
Small Intestine ◽  
Goblet Cells ◽  
Rat Small Intestine ◽  
Quantitative Changes

scholarly journals Are the Matrix Metalloproteinases 2 and 9 beeing expressed in the mucosa of the rat small intestine during its intrauterine and postnatal life development ?

2019 ◽  
Author(s):  
Camila Audrey dos Reis ◽  
José Rosa Gomes
Keyword(s):  
Innate Immunity ◽  
Small Intestine ◽  
Mast Cells ◽  
Lamina Propria ◽  
Goblet Cells ◽  
Postnatal Life ◽  
Rat Small Intestine ◽  
The Matrix ◽  
Intestine Mucosa ◽  
Masson Trichrome Staining

ABSTRACTMMP-2 and MMP-9 are proteins with well stablished roles on the remodeling of tissue during morphogenesis. This study aimed to evaluate the activity and expression of the MMP-2 and MMP-9 in the rat small intestine mucosa layer on 15th and 18th days of intratuterine life (i.u.) and at 3rd, 10th, 17th, 25th, and 32th days after birth (a.b.). Samples were submitted to zimography, immunohistochemistry methods and Masson trichrome staining. Results showed that MMP-2 and MMP-9 were not expressed in the small intestine epithelium during intrauterine life. MMP-9 was immunolocalized in the villi goblet cells and in the lamina propria in cells identified as being the mast cells (a.b.). We concluded that in the i.u. and a.b. life the MMP-2 and MMP-9 were not expressed in the small intestine epithelium. However, after birth, because MMP-9 was expressed in the mast cels present in the lamina propria it may be involved in the remodeling process of the innate immunity that occurs during the small intestine development.

scholarly journals Transient expression of [D-Ala2] deltorphin I-like immunoreactivity in prenatal rat small intestine.

1994 ◽  
Vol 42 (10) ◽  
pp. 1377-1381 ◽  
Author(s):  
J Matsui ◽  
M Fujimiya ◽  
S Matsui ◽  
Y Amakata ◽  
T Renda ◽  
...  
Keyword(s):  
Small Intestine ◽  
Electron Microscopic Examination ◽  
Goblet Cells ◽  
Postnatal Period ◽  
Intestinal Lumen ◽  
Positive Staining ◽  
Rat Small Intestine ◽  
Electron Microscopic ◽  
Apical Cytoplasm ◽  
Undifferentiated Cells

We studied the distribution of immunoreactive elements for [D-Ala2] deltorphin I (DADTI), a delta-opioid receptor ligand, in fetal and postnatal rat small intestine. DADTI-like immunoreactive cells were detected transiently on embryonic Days 20 and 21. Electron microscopic examination revealed that positive staining occurred in mucous epithelial cells, either mature goblet cells or undifferentiated cells containing only a few mucous granules. Positive immunoreaction products in mature goblet cells were confined in their apical cytoplasm to the luminal parts of mucous granule aggregates. The result suggests that a DADTI-like molecule(s) is synthesized in rat intestinal goblet cells and is secreted in a diacrine fashion into the intestinal lumen at a late fetal period. The molecule(s) thus secreted may be important for the intestine of rats just before birth, because DADTI-like immunopositive goblet cells are no longer seen at any postnatal period.

Goblet cells and intestinal Alkaline phosphatase expression (IAP) during the development of the rat small intestine

2017 ◽  
Vol 119 (1) ◽  
pp. 71-77 ◽  
Author(s):  
José Rosa Gomes ◽  
Laís Costa Ayub ◽  
Camila Audrey dos Reis ◽  
Miriam Joice Machado ◽  
Jéssica da Silva ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Small Intestine ◽  
Goblet Cells ◽  
Rat Small Intestine ◽  
Intestinal Alkaline Phosphatase ◽  
Alkaline Phosphatase Expression

Dimethylthiourea pretreatment inhibits endotoxin-induced compound exocytosis in goblet cells and plasma leakage of rat small intestine

2009 ◽  
Vol 59 (2) ◽  
pp. 127-139 ◽  
Author(s):  
S. P. Liu ◽  
C. Y. Chang ◽  
W. H. Huang ◽  
Y. S. Fu ◽  
D. Chao ◽  
...  
Keyword(s):  
Small Intestine ◽  
Goblet Cells ◽  
Rat Small Intestine ◽  
Compound Exocytosis ◽  
Plasma Leakage

In situ demonstration of migration of dendritic cells released from rat small intestine to mesenteric lymph nodes

2001 ◽  
Vol 120 (5) ◽  
pp. A183-A183
Author(s):  
H KOBAYASHI ◽  
H NAGATA ◽  
S MIURA ◽  
T AZUMA ◽  
H SUZUKI ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Small Intestine ◽  
Lymph Nodes ◽  
Rat Small Intestine ◽  
Mesenteric Lymph Nodes ◽  
Mesenteric Lymph

Anti-inflammatory effect of STW 5, STW 6 and isolated extracts on the rat small intestine

2008 ◽  
Vol 29 (S 1) ◽  
Author(s):  
K Nieber ◽  
S Michael ◽  
K Grötzinger ◽  
JW Rauwald ◽  
O Kelber
Keyword(s):  
Small Intestine ◽  
Rat Small Intestine ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Oral vanadate reduces Na(+)-dependent glucose transport in rat small intestine

Diabetes ◽  
1993 ◽  
Vol 42 (8) ◽  
pp. 1126-1132 ◽  
Author(s):  
K. L. Madsen ◽  
V. M. Porter ◽  
R. N. Fedorak
Keyword(s):  
Small Intestine ◽  
Glucose Transport ◽  
Rat Small Intestine

Histological examination of rat small intestine after surgical removal of obturation small bowel obstruction and peptide stimulation of lymph flow

2018 ◽  
pp. 157-162
Author(s):  
А.А. Коваленко ◽  
Г.П. Титова ◽  
В.К. Хугаева
Keyword(s):  
Small Intestine ◽  
Surgical Treatment ◽  
Survival Rate ◽  
Small Bowel ◽  
Goblet Cells ◽  
Intestinal Wall ◽  
Structure And Function ◽  
Lymph Flow ◽  
Intestinal Lumen ◽  
And Function

Оперативное лечение различных заболеваний кишечника сопровождается осложнениями в виде нарушений микроциркуляции в области анастомоза кишки. Ранее нами показана способность лимфостимуляторов пептидной природы восстанавливать нарушенную микроциркуляцию, что послужило основой для настоящего исследования. Цель работы - оценка влияния стимуляции лимфотока в стенке кишки на процессы восстановления микроциркуляции, структуры и функции тонкой кишки в области оперативного вмешательства. Методика. В экспериментах на наркотизированных крысах (хлоралгидрат в дозе 0,6 г/кг в 0,9% растворе NaCl) моделировали различные поражения тонкой кишки (наложение лигатуры, перевязка 1-3 брыжеечных артерий, перекрут петли кишки вокруг оси брыжейки, сочетание нескольких видов повреждений). Резекция поврежденного участка через 1 сут. с последующим созданием тонкокишечного анастомоза завершалась орошением операционного поля раствором пептида-стимулятора лимфотока (40 мкг/кг массы животного в 1 мл 0,9% раствора NaCl). На 7-е сут. после операции проводили гистологическое исследование фрагмента кишки в области анастомоза. Результаты. На 7-е сут. после резекции у выживших животных (летальность вследствие кишечной непроходимости составляла 30%) имеют место морфологические признаки острых сосудистых нарушений стенки кишки, изменений кровеносных и лимфатических микрососудов, интерстициальный отек всех слоев стенки кишки, дилатация просвета кишки, повреждение всасывающего эпителия ворсин с истончением щеточной каемки клеток, морфологические признаки гиперфункции бокаловидных клеток. Использование лимфостимулятора пептидной природы после операции увеличивало выживаемость животных на 24%. У части животных отмечалось уменьшение расширения просвета кишки, у других практически полная его нормализация. Восстанавливалась форма кишечных ворсин и распределение бокаловидных клеток. Отсутствовали признаки внутриклеточного и межмышечного отека. Отмечено умеренное полнокровие венул. Заключение. Использование лимфостимулятора при хирургическом лечении кишечной непроходимости увеличивает выживаемость животных на 24% по сравнению с контролем, способствует более раннему восстановлению структуры и функции тонкой кишки. Полученные результаты свидетельствуют о перспективности использования стимуляции лимфотока при операциях на кишечнике. Surgical treatment of bowel diseases is associated with complications that cause microcirculatory disturbances in the anastomosis area and may lead to a fatal outcome. This study was based on our previous finding that peptide-type lymphatic stimulators are able to restore impaired microcirculation. The aim of this work was stimulating the lymph flow in the intestinal wall to facilitate recovery of microcirculation, structure and function of the small intestine in the area of surgical intervention. Methods. In experiments on anesthetized rats (0.6 g/kg chloral hydrate in 0.9% NaCl), various small bowel lesions were modeled (bowel ligation, ligation of 1-3 mesenteric arteries, gut torsion, combination of several lesion types). In 24 h, the damaged area was resected, and a small intestine anastomosis was creased. The surgery was completed with irrigation of the operative field with a solution of lymph flow stimulating peptide (40 мg/kg body weight in 1 ml of 0.9% NaCl). A gut fragment from the anastomosis area was examined histologically on day 7 after the surgery. Results. On the 7th day after removing the intestinal obstruction, the surviving animals (lethality 30%) had morphological signs of acute vascular disorders in the intestinal wall; changes in blood and lymphatic microvessels; interstitial edema of all intestinal wall layers; dilatation of the intestinal lumen; damage to the absorptive epithelium of villi with thinning of the brush border, and hyperfunction of mucous (goblet) cells. The use of the peptide after surgery increased the survival rate of animals by 24% and provided a smaller dilatation of the intestinal lumen in some animals. In other animals, the lumen recovered. The shape of intestinal villi and distribution of goblet cells were restored. Signs of intracellular and intermuscular edema were absent. Moderate venular congestion was noticed. Conclusion. Using the lymphatic stimulator in surgical treatment of intestinal obstruction increases the survival rate of animals by 24% compared to the control, facilitates earlier restoration of the small intestine structure and function. The obtained results indicated the effectiveness of lymphatic stimulation in intestinal surgery.

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