Direct signaling from astrocytes to neurons in cultures of mammalian brain cells

Science ◽  
1994 ◽  
Vol 263 (5154) ◽  
pp. 1768-1771 ◽  
Author(s):  
M Nedergaard
Keyword(s):  
Mammalian Brain ◽  
Brain Cells

scholarly journals The Chemically Synthesized Ageladine A-Derivative LysoGlow84 Stains Lysosomes in Viable Mammalian Brain Cells and Specific Structures in the Marine Flatworm Macrostomum lignano

Marine Drugs ◽  
2015 ◽  
Vol 13 (2) ◽  
pp. 920-935 ◽  
Author(s):  
Thorsten Mordhorst ◽  
Sushil Awal ◽  
Sebastian Jordan ◽  
Charlotte Petters ◽  
Linda Sartoris ◽  
...  
Keyword(s):  
Mammalian Brain ◽  
Brain Cells ◽  
Macrostomum Lignano

scholarly journals Mitotic defects lead to neuronal aneuploidy and apoptosis in frontotemporal lobar degeneration caused by MAPT mutations

2018 ◽  
Vol 29 (5) ◽  
pp. 575-586 ◽  
Author(s):  
Julbert Caneus ◽  
Antoneta Granic ◽  
Rosa Rademakers ◽  
Dennis W. Dickson ◽  
Christina M. Coughlan ◽  
...  
Keyword(s):  
Mitotic Spindle ◽  
Frontotemporal Lobar Degeneration ◽  
Gene Dosage ◽  
Human Lymphocytes ◽  
Chromosome Instability ◽  
Mammalian Brain ◽  
Brain Cells ◽  
Brain Neurons ◽  
Normal Human ◽  
Mutant Forms

Mutant Tau (MAPT) can lead to frontotemporal lobar degeneration (FTLD). Previous studies associated MAPT mutations and altered function with aneuploidy and chromosome instability in human lymphocytes and in Drosophila development. Here we examine whether FTLD-causing mutations in human MAPT induce aneuploidy and apoptosis in the mammalian brain. First, aneuploidy was found in brain cells from MAPT mutant transgenic mice expressing FTLD mutant human MAPT. Then brain neurons from mice homozygous or heterozygous for the Tau (Mapt) null allele were found to exhibit increasing levels of aneuploidy with decreasing Tau gene dosage. To determine whether aneuploidy leads to neurodegeneration in FTLD, we measured aneuploidy and apoptosis in brain cells from patients with MAPT mutations and identified both increased aneuploidy and apoptosis in the same brain neurons and glia. To determine whether there is a direct relationship between MAPT-induced aneuploidy and apoptosis, we expressed FTLD-causing mutant forms of MAPT in karyotypically normal human cells and found that they cause aneuploidy and mitotic spindle defects that then result in apoptosis. Collectively, our findings reveal a neurodegenerative pathway in FTLD-MAPT in which neurons and glia exhibit mitotic spindle abnormalities, chromosome mis-segregation, and aneuploidy, which then lead to apoptosis.

The differential distribution of beta tubulin mRNAs in individual mammalian brain cells

1985 ◽  
Vol 27 (3) ◽  
pp. 205-214 ◽  
Author(s):  
W. Sue T. Griffin ◽  
Michael A. Alejos ◽  
Erica J. Cox ◽  
Marcelle R. Morrison
Keyword(s):  
Mammalian Brain ◽  
Brain Cells ◽  
Differential Distribution ◽  
Beta Tubulin

scholarly journals MEASURING MICROFLUIDIC SYSTEM FOR THE MAMMALIAN BRAIN CELLS CULTIVATION

2019 ◽  
Vol 29 (4) ◽  
pp. 51-56
Author(s):  
A. S. Yakimov ◽  
◽  
E. D. Osipova ◽  
A. V. Morgun ◽  
E. B. Boytsova ◽  
...  
Keyword(s):  
Microfluidic System ◽  
Mammalian Brain ◽  
Brain Cells

scholarly journals Identification of potential astrocytes in the teleost brain

2021 ◽  
Author(s):  
Germaine Jia Hui Tan ◽  
Kathleen Wei Ben Cheow ◽  
May Si Min Ho ◽  
Suresh J Jesuthasan
Keyword(s):  
In Situ Hybridization ◽  
Glial Fibrillary Acidic Protein ◽  
Glial Cells ◽  
Acidic Protein ◽  
Mammalian Brain ◽  
Brain Cells ◽  
Transcriptome Data ◽  
Zebrafish Brain ◽  
Teleost Brain

Astrocytes are abundant star-shaped glial cells in the mammalian brain, with essential roles in metabolism, development, homeostasis, response to injury, behavior, and learning. Surprisingly, most regions of the teleost brain are thought to lack astrocytes, based primarily on the use of GFAP (glial fibrillary acidic protein) as a marker. Here, drawing on recent evidence that astrocytes are molecularly heterogeneous, we propose that astrocytes exist in the teleost brain, albeit of the olig2 subtype. Highly branched cells are present throughout the zebrafish brain, as shown here in Tg(sox10:EGFP) fish and previously in Tg(olig2:GFP) fish. Transcriptome data indicates the presence of brain cells that are olig2 and sox10 positive, which also express the astrocyte markers sox9b, sparcl1 and slc1a2b but lack gfap and the oligodendrocyte marker mbp. In situ hybridization confirms that stellate sox10:EGFP cells express olig2 and sox9b, while immunofluorescence indicates that they lack HuC/D and GFAP. We suggest that these cells be classified as astrocytes as this may more accurately reflect their functions.

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