Asymmetrically permeable membrane channels in cell junction

Science ◽  
1980 ◽  
Vol 207 (4432) ◽  
pp. 771-773 ◽  
Author(s):  
J. Flagg-Newton ◽  
W. Loewenstein
Keyword(s):  
Cell Junction ◽  
Membrane Channels ◽  
Permeable Membrane

Amphotericin B Covalent Dimers Forming Sterol-Dependent Ion-Permeable Membrane Channels

2002 ◽  
Vol 124 (16) ◽  
pp. 4180-4181 ◽  
Author(s):  
Nobuaki Matsumori ◽  
Nahoko Yamaji ◽  
Shigeru Matsuoka ◽  
Tohru Oishi ◽  
Michio Murata
Keyword(s):  
Amphotericin B ◽  
Membrane Channels ◽  
Permeable Membrane

CFD CHARACTERIZATION OF FLOW PATTERN AROUND ENDOTHELIAL CELL IN DENGUE INFECTION WITH PLASMA LEAKAGE

2015 ◽  
Vol 76 (7) ◽  
Author(s):  
Nur Kaliwantoro ◽  
Marsetyawan HNE Soesatyo ◽  
Indarto Indarto ◽  
Mohammad Juffrie ◽  
Rini Dharmastiti ◽  
...  
Keyword(s):  
Shear Stress ◽  
Endothelial Cell ◽  
Dengue Virus ◽  
Fluid Dynamic ◽  
Dengue Infection ◽  
Leading Edge ◽  
Cell Junction ◽  
Computational Domain ◽  
Permeable Membrane ◽  
Plasma Leakage

Plasma leakage is the pathological hall mark in dengue infection and may cause fatal condition to the patients. In this paper, the CFD (computational fluid dynamic) model is adopted to characterize the flow on the endothelial cells surface with plasma leakage based on in vitro experiments of HUVEC (human umbilical vein endothelial cell) culture on the permeable membrane. The computational domain used is a simplified model of single cell. At the leading edge of the domain and among the membranes, the gaps are modeled as a representation of cell-cell junction breakdown caused by dengue virus infection.  The result shows that at the leading edge , the fluid starts to move more quickly and increases to the maximum value at the middle of the cell and then drops to zero at the trailing edge. From the physical point of view, this result describes that there is a variation of the values of the wall shear stress due to the velocity gradient. These results can be considered as a first step to develop the ways of the prevention of the dengue infection through manipulation the shear stress to reduce the potency of dengue virus to attach the cell surface.  

Mode of action of colicin Ib Formation of ion-permeable membrane channels

1981 ◽  
Vol 645 (1) ◽  
pp. 137-142 ◽  
Author(s):  
Craig A. Weaver ◽  
Bruce L. Kagan ◽  
Alan Finkelstein ◽  
Jordan Konisky
Keyword(s):  
Mode Of Action ◽  
Membrane Channels ◽  
Permeable Membrane

Quantum jumps of conductance during formation of membrane channels at cell–cell junction

Nature ◽  
1978 ◽  
Vol 274 (5667) ◽  
pp. 133-136 ◽  
Author(s):  
W. R. Loewenstein ◽  
Y. Kanno ◽  
S. J. Socolar
Keyword(s):  
Cell Junction ◽  
Membrane Channels ◽  
Quantum Jumps ◽  
Cell Cell

Calcium ion produces graded changes in permeability of membrane channels in cell junction

Nature ◽  
1977 ◽  
Vol 267 (5612) ◽  
pp. 625-627 ◽  
Author(s):  
BIRGIT ROSE ◽  
IAN SIMPSON ◽  
WERNER R. LOEWENSTEIN
Keyword(s):  
Calcium Ion ◽  
Cell Junction ◽  
Membrane Channels

Freeze-etch observations on the tight junctions of sertoli cells grown in organ culture with FSH

1978 ◽  
Vol 36 (2) ◽  
pp. 340-341
Author(s):  
Rita Meyer ◽  
Zoltan Posalaky ◽  
Dennis Mcginley
Keyword(s):  
Organ Culture ◽  
Tight Junctions ◽  
Sertoli Cell ◽  
Germ Cells ◽  
Sertoli Cells ◽  
Barrier Function ◽  
Cell Junction ◽  
Intramembranous Particles ◽  
Junctional Complexes ◽  
Oriented Parallel

The Sertoli cell tight junctional complexes have been shown to be the most important structural counterpart of the physiological blood-testis barrier. In freeze etch replicas they consist of extensive rows of intramembranous particles which are not only oriented parallel to one another, but to the myoid layer as well. Thus the occluding complex has both an internal and an overall orientation. However, this overall orientation to the myoid layer does not seem to be necessary to its barrier function. The 20 day old rat has extensive parallel tight junctions which are not oriented with respect to the myoid layer, and yet they are inpenetrable by lanthanum. The mechanism(s) for the control of Sertoli cell junction development and orientation has not been established, although such factors as the presence or absence of germ cells, and/or hormones, especially FSH have been implicated.

Electrophysiological Characterization of Transport Across Outer Membrane Channels from Gram-Negative Bacteria in Their Native Environment

2019 ◽  
Author(s):  
Jiajun Wang ◽  
Rémi Terrasse ◽  
Jayesh Arun Bafna ◽  
Lorraine Benier ◽  
Mathias Winterhalter
Keyword(s):  
Outer Membrane ◽  
Lipid Membrane ◽  
Membrane Vesicles ◽  
Outer Membrane Vesicles ◽  
Multi Drug Resistance ◽  
Gram Negative Bacteria ◽  
Membrane Channels ◽  
Gram Negative ◽  
Electrophysiological Characterization

Multi-drug resistance in Gram-negative bacteria is often associated with low permeability of the outer membrane. To investigate the role of membrane channels in the uptake of antibiotics, we extract, purify and reconstitute them into artificial planar membranes. To avoid this time-consuming procedure, here we show a robust approach using fusion of native outer membrane vesicles (OMV) into planar lipid bilayer which moreover allows also to some extend the characterization of membrane protein channels in their native environment. Two major membrane channels from <i>Escherichia coli</i>, OmpF and OmpC, were overexpressed from the host and the corresponding OMVs were collected. Each OMV fusion revealed surprisingly single or only few channel activities. The asymmetry of the OMV´s translates after fusion into the lipid membrane with the LPS dominantly present at the side of OMV addition. Compared to conventional reconstitution methods, the channels fused from OMVs containing LPS have similar conductance but a much broader distribution. The addition of Enrofloxacin on the LPS side yields somewhat higher association (<i>k<sub>on</sub></i>) and lower dissociation (<i>k<sub>off</sub></i>) rates compared to LPS-free reconstitution. We conclude that using outer membrane vesicles is a fast and easy approach for functional and structural studies of membrane channels in the native membrane.

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