Human cell hybrids: analysis of transformation and tumorigenicity

Science ◽  
1982 ◽  
Vol 215 (4530) ◽  
pp. 252-259 ◽  
Author(s):  
E. Stanbridge ◽  
C. Der ◽  
C. Doersen ◽  
R. Nishimi ◽  
D. Peehl ◽  
...  
Keyword(s):  
Human Cell ◽  
Cell Hybrids

scholarly journals Human Chromosomes 9, 12, and 15 Contain the Nucleation Sites of Stress-Induced Nuclear Bodies

2002 ◽  
Vol 13 (6) ◽  
pp. 2069-2079 ◽  
Author(s):  
Marco Denegri ◽  
Daniela Moralli ◽  
Mariano Rocchi ◽  
Marco Biggiogera ◽  
Elena Raimondi ◽  
...  
Keyword(s):  
Heat Shock ◽  
Human Cell ◽  
Hela Cells ◽  
Heat Shock Factor ◽  
Nuclear Bodies ◽  
Chromosome 9 ◽  
Heat Shock Factor 1 ◽  
Human Chromosomes ◽  
Cell Hybrids ◽  
Nucleation Sites

We previously reported the identification of a novel nuclear compartment detectable in heat-shocked HeLa cells that we termed stress-induced Src-activated during mitosis nuclear body (SNB). This structure is the recruitment center for heat shock factor 1 and for a number of RNA processing factors, among a subset of Serine-Arginine splicing factors. In this article, we show that stress-induced SNBs are detectable in human but not in hamster cells. By means of hamster>human cell hybrids, we have identified three human chromosomes (9, 12, and 15) that are individually able to direct the formation of stress bodies in hamster cells. Similarly to stress-induced SNB, these bodies are sites of accumulation of hnRNP A1-interacting protein and heat shock factor 1, are usually associated to nucleoli, and consist of clusters of perichromatin granules. We show that the p13-q13 region of human chromosome 9 is sufficient to direct the formation of stress bodies in hamster>human cell hybrids. Fluorescence in situ hybridization experiments demonstrate that the pericentromeric heterochromatic q12 band of chromosome 9 and the centromeric regions of chromosomes 12 and 15 colocalize with stress-induced SNBs in human cells. Our data indicate that human chromosomes 9, 12, and 15 contain the nucleation sites of stress bodies in heat-shocked HeLa cells.

Identification of a deletion in the mismatch repair gene, MSH2, using mouse-human cell hybrids monosomal for chromosome 2

2003 ◽  
Vol 63 (3) ◽  
pp. 215-218 ◽  
Author(s):  
RE Pyatt ◽  
H Nakagawa ◽  
H Hampel ◽  
M Sedra ◽  
MB Fuchik ◽  
...  
Keyword(s):  
Mismatch Repair ◽  
Human Cell ◽  
Mismatch Repair Gene ◽  
Chromosome 2 ◽  
Repair Gene ◽  
Cell Hybrids

Reduction of Caveolin-1 Expression in Tumorigenic Human Cell Hybrids

1998 ◽  
Vol 124 (2) ◽  
pp. 383-388 ◽  
Author(s):  
T. Suzuki ◽  
Y. Suzuki ◽  
K. Hanada ◽  
A. Hashimoto ◽  
J. L. Redpath ◽  
...  
Keyword(s):  
Human Cell ◽  
Caveolin 1 ◽  
Cell Hybrids

Loss of Suppressor Loci on Chromosomes 11 and 14 May Be Required for Radiation-Induced Neoplastic Transformation of HeLa × Skin Fibroblast Human Cell Hybrids

1998 ◽  
Vol 149 (3) ◽  
pp. 246 ◽  
Author(s):  
Marc S. Mendonca ◽  
Kelly Howard ◽  
Clare L. Fasching ◽  
Daphne L. Farrington ◽  
Lael A. Desmond ◽  
...  
Keyword(s):  
Human Cell ◽  
Skin Fibroblast ◽  
Neoplastic Transformation ◽  
Cell Hybrids ◽  
Radiation Induced
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