Monoclonal antibody to acetylcholine receptor: cell line established from thymus of patient with Myasthenia gravis

Science ◽  
1982 ◽  
Vol 215 (4535) ◽  
pp. 995-997 ◽  
Author(s):  
I Kamo ◽  
S Furukawa ◽  
A Tada ◽  
Y Mano ◽  
Y Iwasaki ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Myasthenia Gravis ◽  
Cell Line ◽  
Acetylcholine Receptor ◽  
Receptor Cell

scholarly journals Heterogeneity of acetylcholine receptor autoantibody-mediated complement activity in patients with myasthenia gravis.

2021 ◽  
Author(s):  
Abeer H Obaid ◽  
Chryssa Zografou ◽  
Douangsone D Vadysirisack ◽  
Bailey Munro-Sheldon ◽  
Miriam L Fichtner ◽  
...  
Keyword(s):  
Myasthenia Gravis ◽  
Cell Line ◽  
Acetylcholine Receptor ◽  
Disease Burden ◽  
Hek293t Cell ◽  
Live Cell ◽  
Complement Activity ◽  
Regulator Genes ◽  
Complement Regulator ◽  
Hek293t Cell Line

Background: Autoantibodies targeting the acetylcholine receptor (AChR) in the serum of myasthenia gravis (MG) patients are broadly polyclonal and heterogeneous in their pathogenic capacity. Specifically, AChR autoantibody-mediated pathology occurs through three mechanisms that include complement-directed tissue damage, blocking of the acetylcholine binding site on the AChR, and modulation (internalization) of the AChR. Clinical assays used for diagnosis and prognosis measure only AChR autoantibody binding and they provide weak association with disease burden, thereby limiting understanding of mechanistic heterogeneity, and monitoring therapeutic response. Objective: To develop an in-vitro cell-based assay that measures AChR autoantibody-mediated complement membrane attack complex (MAC) formation. Methods: A HEK293T cell line, which is commonly used for live cell-based AChR autoantibody binding assays, was modified such that the expression of the complement regulator genes (CD46, CD55 and CD59) were disrupted using CRISPR/Cas9 genome editing. This modified cell line was used to measure serum AChR autoantibody-mediated complement MAC formation via flow cytometry. Results: AChR autoantibody-mediated MAC formation required the use of a modified HEK293T cell line in which the surface expression of three complement regulator genes was absent. Serum samples (n=155) from 97 clinically confirmed AChR patients were tested along with 32 healthy donor (HD) samples; the MG cohort included a wide range of disease burden and AChR autoantibody titer. AChR autoantibodies were detected in 139 of the 155 (89.7%) AChR patient samples via a live cell-based assay. Of the 139 AChR positive samples, autoantibody-mediated MAC formation was detected in 83 (59.7%), while no autoantibodies or MAC formation was detected in samples from the HD group. Autoantibody-mediated MAC formation positively associated with autoantibody binding in most MG patient samples. However, a subset displayed a disassociation between binding and MAC formation. Conclusions: We demonstrate the development of a novel assay for evaluating AChR autoantibody-mediated complement activity. It is anticipated that this assay will afford a deeper understanding of the heterogeneous disease pathology and allow for the identification of MG patients who may benefit from complement inhibitor therapy.

Plasma from myasthenia gravis patients reduces acetylcholine receptor agonist-induced Na+ flux into TE671 cell line

1988 ◽  
Vol 19 (1-2) ◽  
pp. 141-148 ◽  
Author(s):  
Bethan Lang ◽  
Gail Richardson ◽  
Julia Rees ◽  
Angela Vincent ◽  
John Newsom-Davis
Keyword(s):  
Myasthenia Gravis ◽  
Cell Line ◽  
Acetylcholine Receptor ◽  
Receptor Agonist ◽  
Te671 Cell

Therapeutic Immunoadsorption of Acetylcholine Receptor Antibodies in Myasthenia Gravis

1988 ◽  
Vol 540 (1 Advances in N) ◽  
pp. 554-556 ◽  
Author(s):  
TAKESHI SATO ◽  
YASUNORI ISHIGAKI ◽  
TADATOSHI KOMIYA ◽  
HIROSHI TSUDA
Keyword(s):  
Myasthenia Gravis ◽  
Acetylcholine Receptor ◽  
Receptor Antibodies

Subunit specificity of the acetylcholine receptor antibodies in double seropositive myasthenia gravis

2021 ◽  
Author(s):  
Vasiliki Zouvelou ◽  
Maria Michail ◽  
Maria Belimezi ◽  
Paraskevi Zisimopoulou
Keyword(s):  
Myasthenia Gravis ◽  
Acetylcholine Receptor ◽  
Receptor Antibodies
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