DNA strand breakage in human leukocytes exposed to a tumor promoter, phorbol myristate acetate

Science ◽  
1982 ◽  
Vol 215 (4537) ◽  
pp. 1247-1249 ◽  
Author(s):  
H. Birnboim
Keyword(s):  
Phorbol Myristate Acetate ◽  
Tumor Promoter ◽  
Myristate Acetate ◽  
Human Leukocytes ◽  
Strand Breakage ◽  
Dna Strand ◽  
Dna Strand Breakage

Factors which affect DNA strand breakage in human leukocytes exposed to a tumor promoter, phorbol myristate acetate

1982 ◽  
Vol 60 (11) ◽  
pp. 1359-1366 ◽  
Author(s):  
H. C. Birnboim
Keyword(s):  
Hydrogen Peroxide ◽  
Dna Damage ◽  
Phorbol Myristate Acetate ◽  
Respiratory Burst ◽  
Superoxide Anion ◽  
Tumor Promoter ◽  
Myristate Acetate ◽  
Strand Breakage ◽  
Dna Strand ◽  
Dna Strand Breakage

We have recently reported that phorbol myristate acetate (PMA) induces extensive DNA strand break damage in human peripheral blood leukocytes. The mechanism of action involves superoxide anion and hydrogen peroxide which are generated by phagocytes during the "respiratory burst." In this report, we describe the effect of various inhibitors and scavengers on PMA-induced DNA damage. Azide and cyanide greatly increased the level of damage; sulfhydryl compounds (glutathione, cysteine, and cysteamine) and ascorbate markedly decreased the level of damage. Hydroxyl radical scavengers such as dimethyl sulfoxide (DMSO) and glycerol also decreased the level of damage but apparently did so by inhibiting the respiratory burst. Diethyldithiocarbamate (DDC) increased the level of DNA damage at low concentrations (<1 mM), but decreased DNA damage at ≥1 mM. The results are consistent with a mechanism involving superoxide anion and hydrogen peroxide, but the precise reaction (free radical or enzymatic) responsible for DNA strand breakage has not been determined. The PMA-stimulated phagocyte is an interesting model system for looking at "active oxygen" mediated DNA damage and factors which influence it.

scholarly journals Synthesis and DNA Strand Breakage Activity of Some 1,4-Diazepines

2003 ◽  
Vol 51 (1) ◽  
pp. 27-31 ◽  
Author(s):  
Nobuko Mibu ◽  
Miho Yukawa ◽  
Nobuhiro Kashige ◽  
Yukiko Iwase ◽  
Yoshinobu Goto ◽  
...  
Keyword(s):  
Strand Breakage ◽  
Dna Strand ◽  
Dna Strand Breakage

Genetic effects of mercury contamination on aquatic snail populations: Allozyme genotypes and DNA strand breakage

2002 ◽  
Vol 21 (3) ◽  
pp. 584-589 ◽  
Author(s):  
Michael J. Benton ◽  
Michelle L. Malott ◽  
Jan Trybula ◽  
Deborah M. Dean ◽  
Sheldon I. Guttman
Keyword(s):  
Genetic Effects ◽  
Mercury Contamination ◽  
Strand Breakage ◽  
Dna Strand ◽  
Dna Strand Breakage
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