Goldfish retina: a correlate between cone activity and morphology of the horizontal cell in clone pedicules

Science ◽  
1979 ◽  
Vol 204 (4400) ◽  
pp. 1436-1438 ◽  
Author(s):  
J. Raynauld ◽  
Laviolette ◽  
H. Wagner
Keyword(s):  
Horizontal Cell ◽  
Cone Activity ◽  
Goldfish Retina

scholarly journals Horizontal-cell gap junction in the goldfish retina: area and density of particles as revealed by complementary freeze replicas.

1991 ◽  
Vol 54 (2) ◽  
pp. 181-188 ◽  
Author(s):  
Hiroshi WASHIOKA ◽  
Hiroshi WATANABE ◽  
Koroku NEGISHI ◽  
Akira TONOSAKI
Keyword(s):  
Gap Junction ◽  
Horizontal Cell ◽  
Goldfish Retina

3H-adenosine uptake selectively labels rod horizontal cells in goldfish retina

1997 ◽  
Vol 14 (2) ◽  
pp. 207-212 ◽  
Author(s):  
Keith M. Studholme ◽  
Stephen Yazulla
Keyword(s):  
Staining Pattern ◽  
Outer Nuclear Layer ◽  
Horizontal Cell ◽  
Horizontal Cells ◽  
The Other ◽  
Axon Terminals ◽  
Adenosine Uptake ◽  
Double Label ◽  
Injection Methods ◽  
Goldfish Retina

AbstractThere are four types of horizontal cell in the goldfish retina, three cone- and one rod-type. The neurotransmitter of only one type, the H1 (cone) horizontal cell, has been identified as GABA. 3H-adenosine uptake was examined as a possible marker for the other classes of horizontal cell. Isolated goldfish retinae were incubated in 3H-adenosine (10–40 μCi) in HEPES-buffered saline for 30 min, then fixed, embedded in plastic, and processed for light-microscopic autoradiography (ARG). For double-label immuno/ARG studies, l-μm-thick sections were processed for GABA postembed immunocytochemistry, then for ARG. 3H-adenosine uptake was localized to cone photoreceptors, presumed precursor cells in the proximal outer nuclear layer, and to a single, continuous row of horizontal cell bodies in the inner nuclear layer. No uptake was localized to the region of horizontal cell axon terminals. 3H-adenosine uptake did not colocalize with GABA-IR in H1 horizontal cells, but it did colocalize with adenosine deaminase immunoreactivity. It is concluded that 3H-adenosine uptake selectively labels rod horizontal cells in the goldfish retina based on position and staining pattern, which are similar to rod horizontal cells stained by Golgi or HRP injection methods. The use of 3H-adenosine uptake may provide a useful tool to study other properties of rod horizontal cells (i.e. development) as well as provide clues as to the transmitter used by these interneurons.

scholarly journals Circadian clock regulation of cone to horizontal cell synaptic transfer in the goldfish retina

PLoS ONE ◽  
2019 ◽  
Vol 14 (8) ◽  
pp. e0218818
Author(s):  
Christophe Ribelayga ◽  
Stuart C. Mangel
Keyword(s):  
Circadian Clock ◽  
Horizontal Cell ◽  
Goldfish Retina

Spectral sensitivity of the feedback signal from horizontal cells to cones in goldfish retina

1998 ◽  
Vol 15 (5) ◽  
pp. 799-808 ◽  
Author(s):  
D.A. KRAAIJ ◽  
M. KAMERMANS ◽  
H. SPEKREIJSE
Keyword(s):  
Spectral Sensitivity ◽  
Horizontal Cell ◽  
Horizontal Cells ◽  
Feedback Signal ◽  
Cell Systems ◽  
Long Wavelength ◽  
Cell Recording ◽  
Cone System ◽  
Goldfish Retina ◽  
Uv Range

The spectral sensitivity of cones in isolated goldfish retina was determined with whole-cell recording techniques. Three spectral classes of cones were found with maximal sensitivities around 620 nm, 540 nm, and 460 nm. UV-cones were not found because our stimulator did not allow effective stimulation in the UV range. The spectral sensitivity of the cones closely matched the cone photopigment absorption spectra at the long wavelength side of the spectrum, but deviated significantly at shorter wavelengths. Surround stimulation induced an inward current in cones due to feedback from horizontal cells. The spectral sensitivity of this feedback signal was determined in all three cone classes and found to be broader than the spectral sensitivity of the cones recorded from, and to be spectrally nonopponent. These data are consistent with a connectivity scheme between cones and horizontal cells in which the three horizontal cell systems feed back to all cone systems and in which all horizontal cell systems receive input from more than one cone system.

scholarly journals The Size of the horizontal cell receptive fields adapts to the stimulus in the light adapted goldfish retina

1996 ◽  
Vol 36 (24) ◽  
pp. 4105-4119 ◽  
Author(s):  
M. Kamermans ◽  
J. Hark ◽  
J.B.A. Habraken ◽  
H. Spekreijse
Keyword(s):  
Horizontal Cell ◽  
Receptive Fields ◽  
Goldfish Retina

The light-induced reduction of horizontal cell receptive field size in the goldfish retina involves nitric oxide

2011 ◽  
Vol 28 (2) ◽  
pp. 137-144 ◽  
Author(s):  
BRYAN A. DANIELS ◽  
WILLIAM H. BALDRIDGE
Keyword(s):  
Nitric Oxide ◽  
Receptive Field ◽  
Horizontal Cell ◽  
Receptive Fields ◽  
Horizontal Cells ◽  
Field Size ◽  
Nitric Oxide Donor ◽  
Nitric Oxide Synthase Inhibitor ◽  
Receptive Field Size ◽  
Goldfish Retina

AbstractHorizontal cells of the vertebrate retina have large receptive fields as a result of extensive gap junction coupling. Increased ambient illumination reduces horizontal cell receptive field size. Using the isolated goldfish retina, we have assessed the contribution of nitric oxide to the light-dependent reduction of horizontal cell receptive field size. Horizontal cell receptive field size was assessed by comparing the responses to centered spot and annulus stimuli and from the responses to translated slit stimuli. A period of steady illumination decreased the receptive field size of horizontal cells, as did treatment with the nitric oxide donor (Z)-1-[N-(2-aminoethyl)-N-(2-ammonioethyl)amino]diazen-1-ium-1,2-diolate (100μM). Blocking the endogenous production of nitric oxide with the nitric oxide synthase inhibitor, NG-nitro-l-arginine methyl ester (1 mM), decreased the light-induced reduction of horizontal cell receptive field size. These findings suggest that nitric oxide is involved in light-induced reduction of horizontal cell receptive field size.

Organization and development of horizontal cells in the goldfish retina, I: The use of monoclonal antibody AT101

1991 ◽  
Vol 6 (4) ◽  
pp. 357-370 ◽  
Author(s):  
You-Wel Peng ◽  
Dominic Man-Kit lam
Keyword(s):  
Monoclonal Antibody ◽  
Retinal Development ◽  
Horizontal Cell ◽  
Inner Nuclear Layer ◽  
Double Labeling ◽  
Cell Surface Glycoprotein ◽  
Adult Retina ◽  
Cell Axon ◽  
Axon Terminals ◽  
Goldfish Retina

AbstractWe have produced and characterized a monoclonal antibody, AT101, which selectively labels both viable and formaldehyde-fixed horizontal cell axon terminals, but not their somas or axons, of the goldfish (Carassius auratus) retina. The antigen recognized by AT101 appears to be a cell surface glycoprotein with a molecular weight of about 35,000 Daltons, and is present exclusively or predominantly in nervous tissues of all vertebrate species examined. We have used AT101 as a probe to analyze immunocytochemically the organization of horizontal cell axon terminals (HCATs) in the adult goldfish retina, and the emergence and maturation of these terminals during retinal development. Because of continued growth at the retinal margin in adult goldfish, there is a peripheral-to-central gradient in the age of cells, with the most mature in the center and the youngest in the periphery. In the center and near periphery of the adult retina, HCATs have a fusiform morphology and form a dense network in the middle and proximal part of the inner nuclear layer. In the far peripheral retina, the axon terminals appear round or ellipsoid. The retina closest to the retinal margin is devoid of AT101 staining, indicating that either HCATs are absent or the antigen recognized by AT101 is not present on HCATs at this stage. A similar sequence of changes in staining pattern is seen during development. Although AT101 staining can first be demonstrated in the larval retina at 1 month after hatching, it appears mostly as punctate structures. At a later stage, there are round or ellipsoid structures that resemble in morphology and location (in the inner nuclear layer) those found in the far peripheral adult retina. Double-labeling experiments with AT101 and antiserum against tubulin also indicate that AT101 labels the HCATs when they first appear during development. These data suggest that the emergence and maturation of HCAT is a late event in retinal development.

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