Characterization of bacterial growth by means of flow microfluorometry

Science ◽  
1977 ◽  
Vol 198 (4322) ◽  
pp. 1175-1176 ◽  
Author(s):  
J. Bailey ◽  
J Fazel-Makjlessi ◽  
D. McQuitty ◽  
Y. Lee ◽  
J. Allred ◽  
...  
Keyword(s):  
Bacterial Growth ◽  
Flow Microfluorometry

Characterization of bacterial growth and the influence of antibiotics by means of UV resonance Raman spectroscopy

Biopolymers ◽  
2006 ◽  
Vol 82 (4) ◽  
pp. 306-311 ◽  
Author(s):  
U. Neugebauer ◽  
U. Schmid ◽  
K. Baumann ◽  
U. Holzgrabe ◽  
W. Ziebuhr ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Bacterial Growth ◽  
Resonance Raman Spectroscopy ◽  
Resonance Raman ◽  
Uv Resonance Raman ◽  
Uv Resonance Raman Spectroscopy

scholarly journals Isolation and expeditious morphological, biochemical and kinetic characterization of propolis-tolerant ruminal bacteria

2010 ◽  
Vol 39 (9) ◽  
pp. 2048-2054 ◽  
Author(s):  
Odimári Pricila Pires do Prado ◽  
Lucia Maria Zeoula ◽  
Lucimar Pontara Peres de Moura ◽  
Selma Lucy Franco ◽  
Sara Barbosa de Paiva ◽  
...  
Keyword(s):  
Bacterial Growth ◽  
Biochemical Characterization ◽  
Kinetic Characterization ◽  
Bacterial Strains ◽  
Gram Positive ◽  
Ruminal Bacteria ◽  
Characterization Techniques ◽  
Gram Staining

It was aimed in this work to evaluate bacterial strains tolerant to products based on propolis (LLOS) through the isolation, morphological and biochemical characterization techniques in diets with roughage:concentrate ratio 100:0 and 50:50. For roughage diets, the products LLOSC1 and LLOSB3 were evaluated, and for 50:50% diets, the products LLOSC1, LLOSD1, LLOSA2, and LLOSC3, which differed in alcoholic concentrations (1, 2 and 3) and propolis (A, B, C and D) concentrations. The ruminal liquid was anaerobically incubated at 39°C for 6 days in medium containing LLOS. After isolation, the strains were submitted to Gram staining and the bacterial growth was monitored by photospectrometer. It was evaluated the strain growth in the presence of the following subtracts: arabinose, cellulose, glucose, cellobiose, xylose, fructose, and lactose. In roughage diets, strains tolerant to LLOSC1 and LLOSB3 were similar to carbohydrates degradation, except lactose in which LLOSC1 was superior to strains tolerant to LLOSB3. For diets with 50:50 roughage:concentrate ratio, the products LLOSC3 and LLOSA2 stood out because they selected the highest number of strains able to degrade most of the tested carbohydrates. The results suggest that tolerance to propolis is higher in Gram-positive strains with several growth metabolic levels.

scholarly journals Synthesis and Characterization of Selenium Nanoparticles-Lysozyme Nanohybrid System with Synergistic Antibacterial Properties

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Mahsa Vahdati ◽  
Tahereh Tohidi Moghadam
Keyword(s):  
Antibacterial Activity ◽  
Bacterial Growth ◽  
Biomedical Applications ◽  
Antibacterial Properties ◽  
Complex Form ◽  
Vital Role ◽  
Selenium Nanoparticles ◽  
Low Concentrations ◽  
Very High

AbstractIn the light of promising potency of selenium nanoparticles in biomedical applications, this is the first study to report the synergistic antibacterial activity of these nanoparticles and lysozyme. The nanohybrid system was prepared with various concentrations of each component. Resistance of Escherichia coli and Staphylococcus aureus was compared in the presence of individual Nano and Bio counterparts as well as the nanohybrid system. Upon interaction of SeNPs with Lysozyme, the nanohybrid system efficiently enhanced the antibacterial activity compared to the protein. Therefore, SeNPs play an important role in inhibition of bacterial growth at very low concentrations of protein; whereas very high amount of the protein is required to inhibit bacterial growth individually. On the other hand, lysozyme has also played a vital role in antibacterial property of SeNPs, inducing 100% inhibition at very low concentration of each component. Hence, presence of both nano and bio counterparts induced vital interplay in the Nanohybrid system. The aged samples also presented good stability of SeNPs both as the intact and complex form. Results of this effort highlight design of nanohybrid systems with synergistic antibacterial properties to overcome the emerging antibiotic resistance as well as to define fruitful applications in biomedicine and food safety.

scholarly journals Bacteria Exposed to Silver Nanoparticles Synthesized by Laser Ablation in Water: Modelling E. coli Growth and Inactivation

Materials ◽  
2020 ◽  
Vol 13 (3) ◽  
pp. 653 ◽  
Author(s):  
Lucija Krce ◽  
Matilda Šprung ◽  
Ana Maravić ◽  
Polona Umek ◽  
Krešimir Salamon ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Bacterial Growth ◽  
Growth Curves ◽  
Dynamic State ◽  
Lag Phase ◽  
The Novel ◽  
Minimal Inhibitory Concentrations ◽  
E Coli ◽  
Main Effect

This study is aimed to better understand the bactericidal mode of action of silver nanoparticles. Here we present the production and characterization of laser-synthesized silver nanoparticles along with growth curves of bacteria treated at sub-minimal and minimal inhibitory concentrations, obtained by optical density measurements. The main effect of the treatment is the increase of the bacterial apparent lag time, which is very well described by the novel growth model as well as the entire growth curves for different concentrations. The main assumption of the model is that the treated bacteria uptake the nanoparticles and inactivate, which results in the decrease of both the nanoparticles and the bacteria concentrations. The lag assumes infinitive value for the minimal inhibitory concentration treatment. This apparent lag phase is not postponed bacterial growth. It is a dynamic state in which the bacterial growth and death rates are close in value. Our results strongly suggest that the predominant mode of antibacterial action of silver nanoparticles is the penetration inside the membrane.

Demonstration and Partial Characterization of a Bacterial Growth Enhancer in sera

2010 ◽  
Vol 62 (1) ◽  
pp. 90-95
Author(s):  
Kanna Okayama ◽  
Takeshi Honda ◽  
Shigeaki Matsuda ◽  
Tadashi Saito ◽  
Masaya Kawase
Keyword(s):  
Bacterial Growth ◽  
Partial Characterization

scholarly journals Production of antibodies in egg whites of chickens

2021 ◽  
Author(s):  
Angel Justiz Vaillant ◽  
Belkis Ferrer-Cosme
Keyword(s):  
Immune Response ◽  
Affinity Chromatography ◽  
Bacterial Growth ◽  
Statistical Significance ◽  
Protein A ◽  
Egg White ◽  
Primary Immune Response ◽  
Enzyme Linked Immunosorbent Assay

AbstractBackgroundIgM, which participates in the primary immune response, is the primary antibody in egg whites. There is scant information about the production of antibodies in egg whites. This study describes the preparation of antibodies against bacterial antigens.MethodsEnzyme-linked immunosorbent assay (ELISA) was used to detect the presence of anti-egg white antibodies. The antibodies were purified using affinity chromatography. Statistical analyses were performed using SPSS version 22. Statistical significance was set at P<0.05.ResultsLarge amounts of anti-protein A antibodies were produced in chicken egg whites. The generation of anti-SpA antibodies was demonstrated by affinity chromatography from 9 d post-immunization egg white samples. Inhibition of agglutination was observed in samples containing anti-SpA antibodies, and agglutination at the bottom of the wells was observed in the negative samples.ConclusionAnti-protein A antibodies (IgM) were produced in the egg whites of the immunized hens. Bacterial growth in blood agar plates was observed only in specimens plated with egg whites from pre-immunized birds. Protein A-affinity chromatography was helpful for the characterization of anti-protein A antibodies. Inhibition of these antibodies was observed in vitro.

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