Diversity of circumsporozoite antigen genes from two strains of the malarial parasite Plasmodium knowlesi

Science ◽  
1985 ◽  
Vol 229 (4715) ◽  
pp. 779-782 ◽  
Author(s):  
S Sharma ◽  
P Svec ◽  
G. Mitchell ◽  
G. Godson
Keyword(s):  
Plasmodium Knowlesi ◽  
Malarial Parasite ◽  
Parasite Plasmodium

Nuclear and mitochondrial DNA of the primate malarial parasite Plasmodium knowlesi

1985 ◽  
Vol 14 (2) ◽  
pp. 199-209 ◽  
Author(s):  
Donald H. Williamson ◽  
Robert J.M. Wilson ◽  
Paul A. Bates ◽  
Shirley McCready ◽  
Francine Perler ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Plasmodium Knowlesi ◽  
Malarial Parasite ◽  
Parasite Plasmodium

scholarly journals An intracellular simian malarial parasite (Plasmodium knowlesi) induces stage-dependent alterations in membrane phospholipid organization of its host erythrocyte

1987 ◽  
Vol 246 (1) ◽  
pp. 103-108 ◽  
Author(s):  
P Joshi ◽  
G P Dutta ◽  
C M Gupta
Keyword(s):  
Developmental Stages ◽  
Plasmodium Knowlesi ◽  
Membrane Phospholipid ◽  
Malarial Parasite ◽  
Merocyanine 540 ◽  
Parasite Plasmodium ◽  
Normal Monkey ◽  
Infected Cells ◽  
Membrane Isolation ◽  
Different Sources

The membrane phospholipid organization in monkey erythrocytes harbouring different developmental stages of the simian malarial parasite Plasmodium knowlesi was studied using phospholipase A2 from two different sources and Merocyanine 540 as the external-membrane probes. Experiments were done to confirm that the phospholipases did not penetrate into the infected cells or hydrolyse phospholipids during membrane isolation. The parasite-free erythrocyte membrane was isolated by differential centrifugation or by using the cationic beads Affi-Gel 731. The purity of the membranes was established by optical and electron microscopy, and by assaying the parasite-specific enzyme glutamate dehydrogenase. About 10% of the phosphatidylethanolamine and none of phosphatidylserine were hydrolysed by the phospholipases in intact normal monkey erythrocytes. However, accessibility of these aminophospholipids to the enzymes was significantly enhanced in the infected cells under identical conditions. The degree of this enhancement depended on the developmental stage of the intracellular parasite, but not on the parasitaemia levels in the infected monkeys, and increased with the parasite growth inside the cells. Analogously, Merocyanine 540 was found to label the trophozoite- or schizont-infected erythrocytes, but not the ring-infected or normal cells. These results demonstrate that the intracellular malarial parasite produces stage-dependent alterations in the membrane phospholipid organization of its host erythrocyte.

scholarly journals THE AGGLUTINATION OF PLASMODIUM KNOWLESI BY IMMUNE SERUM

1938 ◽  
Vol 67 (6) ◽  
pp. 857-870 ◽  
Author(s):  
Monroe D. Eaton
Keyword(s):  
Plasmodium Knowlesi ◽  
Immune Serum ◽  
Malarial Parasite ◽  
Passive Immunity ◽  
Malarial Infection ◽  
Parasite Plasmodium ◽  
Plasmodium Inui

A specific agglutination of Plasmodium knowlesi detectable both by macroscopic and by microscopic methods is described. Agglutinins for Plasmodium knowlesi appear in the sera of monkeys between 15 and 45 days after the onset of the infection and become progressively stronger as the malarial infection gradually subsides. Agglutinins persist in the sera of chronically infected animals for a year or longer. The sera of animals which have been repeatedly superinfected agglutinate parasites at dilutions as high as 1:1,000. Sera from normal monkeys, from monkeys acutely ill with malaria, and from monkeys chronically infected with a different species of malarial parasite (Plasmodium inui) do not agglutinate Plasmodium knowlesi. Immune serum agglutinates mature intracellular or extracellular parasites but does not agglutinate unparasitized cells or cells containing immature parasites. The relation of these observations to the mechanism of active and passive immunity in monkey malaria is discussed.

scholarly journals Characterization of Simian Malarial Parasite (Plasmodium knowlesi)-induced Putrescine Transport in Rhesus Monkey Erythrocytes

1997 ◽  
Vol 272 (21) ◽  
pp. 13506-13511 ◽  
Author(s):  
Sanjay Singh ◽  
Sunil K. Puri ◽  
Shio K. Singh ◽  
Ragini Srivastava ◽  
Ram C. Gupta ◽  
...  
Keyword(s):  
Rhesus Monkey ◽  
Plasmodium Knowlesi ◽  
Malarial Parasite ◽  
Parasite Plasmodium

Nuclear and mitochondrial DNA of the primate malarial parasite Plasmodium knowlesi

1986 ◽  
Vol 18 (1) ◽  
pp. 123
Author(s):  
Donald H. Williamson ◽  
Robert J.M. Wilson ◽  
Paul A. Bates ◽  
Shirley McCready ◽  
Francine Perler ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Plasmodium Knowlesi ◽  
Malarial Parasite ◽  
Parasite Plasmodium
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