Unusual immunoglobulin gene rearrangement leads to replacement of recombinational signal sequences

Science ◽  
1988 ◽  
Vol 242 (4876) ◽  
pp. 261-263 ◽  
Author(s):  
E Morzycka-Wroblewska ◽  
F. Lee ◽  
S. Desiderio
Keyword(s):  
Gene Rearrangement ◽  
Immunoglobulin Gene ◽  
Signal Sequences ◽  
Immunoglobulin Gene Rearrangement

Immunoglobulin gene rearrangement in B cell deficient mice generated by targeted deletion of the JH locus

1993 ◽  
Vol 5 (6) ◽  
pp. 647-656 ◽  
Author(s):  
Jianzhu Chen ◽  
Mary Trounstine ◽  
Frederick W. Alt ◽  
Faith Young ◽  
Carole Kurahara ◽  
...  
Keyword(s):  
B Cell ◽  
Gene Rearrangement ◽  
Immunoglobulin Gene ◽  
Targeted Deletion ◽  
Immunoglobulin Gene Rearrangement ◽  
Deficient Mice

Clonality of Cutaneous B-Cell Infiltrates Determined by Microdissection and Immunoglobulin Gene Rearrangement

1999 ◽  
Vol 8 (4) ◽  
pp. 176-182 ◽  
Author(s):  
Sabina Signoretti ◽  
Michael Murphy ◽  
Pietro Puddu ◽  
John F. DeCoteau ◽  
Tullio Faraggiana ◽  
...  
Keyword(s):  
B Cell ◽  
Gene Rearrangement ◽  
Immunoglobulin Gene ◽  
Immunoglobulin Gene Rearrangement

scholarly journals Clinical and laboratory characteristics of acute leukemia with the 4;11 translocation

Blood ◽  
1986 ◽  
Vol 67 (3) ◽  
pp. 689-697 ◽  
Author(s):  
J Mirro ◽  
G Kitchingman ◽  
D Williams ◽  
GJ Lauzon ◽  
CC Lin ◽  
...  
Keyword(s):  
Acute Lymphoblastic Leukemia ◽  
Acute Leukemia ◽  
B Cell ◽  
Heavy Chain ◽  
Gene Rearrangement ◽  
Lymphoblastic Leukemia ◽  
Flow Cytometric Analysis ◽  
Terminal Deoxynucleotidyl Transferase ◽  
Immunoglobulin Gene ◽  
Immunoglobulin Gene Rearrangement

Abstract This report describes the clinical and laboratory features of seven cases of acute leukemia associated with the 4;11 chromosomal translocation. All seven children had acute lymphoblastic leukemia by standard morphologic and cytochemical criteria. Leukemic blasts from six of seven patients were terminal deoxynucleotidyl transferase- positive. Immunologic phenotyping suggested the leukemias were of B cell origin; blasts from five patients expressed HLA-DR and p24 (CD-9 antibody), blasts from three patients expressed B4 (CD-19), and blasts from two patients expressed the common acute lymphoblastic leukemia antigen (CD-10). One patient's leukemic blasts contained cytoplasmic immunoglobulin. Analysis of DNA from four of five patients demonstrated additional evidence of B cell differentiation with heavy-chain immunoglobulin gene rearrangement. When DNA from the four patients with heavy-chain immunoglobulin gene rearrangement was analyzed, one patient's DNA demonstrated light-chain immunoglobulin gene rearrangement. However, flow cytometric analysis of blasts from three patients showed the simultaneous expression of the lymphoid-associated antigen B4 (CD-19) and the myeloid-associated antigen My-1 (X-Hapten). Electron microscopic examination of blasts from one patient that expressed both lymphoid- and myeloid-associated antigens demonstrated ultrastructural characteristics of both lineages. These findings suggest that acute leukemia with the t(4;11) abnormality has mixed lineage characteristics as a result of leukemogenesis in a multipotential progenitor cell or aberrant gene expression later in differentiation. Furthermore, serial analysis of karyotype, immunophenotype, and heavy-chain immunoglobulin genes revealed changes in these biologic markers over time, suggesting continued chromosome rearrangement and gene modulation after the leukemogenic event in cells with the t(4;11).

Acute mixed lineage leukemia: clinicopathologic correlations and prognostic significance

Blood ◽  
1985 ◽  
Vol 66 (5) ◽  
pp. 1115-1123 ◽  
Author(s):  
J Mirro ◽  
TF Zipf ◽  
CH Pui ◽  
G Kitchingman ◽  
D Williams ◽  
...  
Keyword(s):  
T Cell ◽  
Cell Surface ◽  
Induction Therapy ◽  
Gene Rearrangement ◽  
Surface Antigens ◽  
Terminal Deoxynucleotidyl Transferase ◽  
Immunoglobulin Gene ◽  
Cell Surface Antigens ◽  
Immunoglobulin Gene Rearrangement ◽  
Dual Staining

Abstract The frequency and clinical significance of acute leukemia displaying both lymphoid and myeloid characteristics was determined in 123 consecutive children using a panel of lineage-associated markers. The leukemic blasts from 18 of 95 children (19%) with the diagnosis of acute lymphoblastic leukemia (ALL) by standard diagnostic criteria expressed myeloid-associated cell surface antigens. Despite immunological evidence of lymphoid differentiation (17 CALLA + and one T cell-associated antigen +) and findings of immunoglobulin gene rearrangement, blasts from these patients reacted with one to five monoclonal antibodies identifying myeloid-associated cell surface antigens (My-1, MCS.2, Mo1, SJ-D1, or 5F1). Dual staining with microsphere-conjugated antibodies and analysis by flow cytometry confirmed that some blasts were simultaneously expressing lymphoid- and myeloid-associated antigens. Conversely, blasts from seven of 28 patients (25%) with acute nonlymphocytic leukemia (ANLL), diagnosed by otherwise standard morphological and cytochemical criteria, expressed lymphoid-associated surface antigens. Dual staining of individual blasts demonstrated simultaneous expression of myeloperoxidase (MPO) (including Auer rods) in association with either T-11, CALLA, or terminal deoxynucleotidyl transferase. Blasts from one patient with ANLL demonstrated T cell receptor gene rearrangement, while blasts from another patient demonstrated characteristics associated with T (T-11), B (CALLA and heavy-chain immunoglobulin gene rearrangement), and myeloid (MPO) lineage. There were no consistent cytogenetic abnormalities, and no patient demonstrated independent leukemic clones. Each patient with typical ALL, except for myeloid-associated antigens, achieved complete remission with conventional induction therapy for ALL. By contrast, three of the seven children with ANLL whose blasts expressed the T-11 surface antigen failed ANLL induction therapy. These three patients subsequently achieved remission with ALL therapy.

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