The CD14 monocyte differentiation antigen maps to a region encoding growth factors and receptors

Science ◽  
1988 ◽  
Vol 239 (4839) ◽  
pp. 497-500 ◽  
Author(s):  
S. Goyert ◽  
E Ferrero ◽  
W. Rettig ◽  
A. Yenamandra ◽  
F Obata ◽  
...  
Keyword(s):  
Growth Factors ◽  
Differentiation Antigen ◽  
Monocyte Differentiation

The CD14 monocyte differentiation antigen maps to a region encoding growth factors and receptors

Science ◽  
1988 ◽  
Vol 239 (4839) ◽  
pp. 497-500 ◽  
Author(s):  
SM Goyert ◽  
E Ferrero ◽  
WJ Rettig ◽  
AK Yenamandra ◽  
F Obata ◽  
...  
Keyword(s):  
Growth Factors ◽  
Differentiation Antigen ◽  
Monocyte Differentiation

STRUCTURAL DIVERSITY AMONG CELLULAR ADHESION RECEPTORS: FIBRINOGEN BINDING IS A NOVEL BIOLOGICAL PROPERTY OF THE MONOCYTE DIFFERENTIATION ANTIGEN OKM1

1987 ◽  
Author(s):  
Dario C Altieri ◽  
Rossella Bader ◽  
Pier M Mannucci
Keyword(s):  
Molecular Size ◽  
Structural Diversity ◽  
Cellular Adhesion ◽  
Differentiation Antigen ◽  
Cell Surface Antigens ◽  
Monocyte Differentiation ◽  
Fibrinogen Receptor ◽  
Biological Adhesion ◽  
Fibrinogen Binding ◽  
Adhesive Proteins

A family of related glycoproteins (GP) mediate the interaction between the circulating adhesive proteins and a variety of cells (cytyoadhesins). In this study we have compared two cell-surface antigens which share the property to bind fibrinogen: the platelet GP IIb/IIIa, prototype of the cytoadhesins, and the receptor for fibrinogen costitutively synthesized by monocytes. Two anti-GP IIb/IIIa monoclonal antibodies (Mabs) (LJP9, LJP5), recognizing functionally distinct epitopes of the GP IIb/IIIa did not react with monocytes nor inhibited 125I-fibrinogen binding to monocytes. Similarly, an Arg-Gly-Asp containing peptide which completely abolished platelet-fibrinogen interaction, had no effect on monocytes. Structurally, the monocyte fibrinogen receptor was dimeric and composed of two subunits with molecular weight (Mr) of 155,000 and 95,000. This structural organization was different from that of the GP IIb/IIIa (Mr= 116,000), but in close analogy with the family of leukocyte differentiation antigens OKM1, LFA-1. Therefore, this possible relationship was investigated. A Mab to OKM1 antigen (10 μg/ml) completely suppressed fibrinogen binding to monocytes while it was ineffective on plateles. Iodinated monocyte lysate subjected to immunoprecipitation with OKM1 Mab (60 μg/ml) showed a dimeric antigen with the same molecular size of the monocyte fibrinogen receptor. Moreover, preclearing of the monocyte lysate with OKM1 Mab removed the immunoprecipitate corresponding to the monocyte fibrinogen receptor. These data indicate that the immunologic differentiation antigen OKM1, in addition to function as a complement receptor, displays also the novel biological adhesion property to mediate the binding of fibrinogen to monocytes.

scholarly journals Serum Molecular Biomarkers in Neuromyelitis Optical and Multiple Sclerosis

2021 ◽  
Author(s):  
Cong-Cong Fu ◽  
Cong Gao ◽  
Hui-Hua Zhang ◽  
Ying-Qing Mao ◽  
Jing-Qiao Lu ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Differentiation Antigen ◽  
Monocyte Differentiation ◽  
Expression Levels ◽  
Chemotactic Protein ◽  
Novel Biomarkers ◽  
Epidermal Growth ◽  
Spectrum Disorders ◽  
Antibody Arrays ◽  
Registry Record

Abstract ObjectiveThe aims of this study were to determine whether the expression levels of serological cytokines could distinguish 1) neuromyelitis optical spectrum disorders (NMOSD) from healthy controls (HCs); and 2) NMOSD patients with and without the aquaporin-4 (AQP-4) antibody biomarker from each other; and 3) NMOSD patients without antibody to AQP-4 from multiple sclerosis (MS). MethodsThe expression levels of 200 proteins in serum from 41 NMOSD (32 with antibodies to AQP-4, 9 without antibodies to AQP-4), 12 MS patients, and 34 HCs were measured using glass-based antibody arrays. In parallel, the correlation between protein expression in NMOSD/MS patients and clinical traits was analyzed with Weighted Gene Co-expression Network Analysis (WGCNA).ResultsThirty-nine serological proteins were differentially expressed in NMOSD patients compared to HCs. 29 differentially-expression proteins (DEPs) were specific to NMOSD whereas 10 of these were observed in NMOSD and MS samples. In addition, there were 15 DEPs between AQP-4-IgG seronegative and AQP-4-IgG seropositive NMOSD patients, and 9 DEPs between NMOSD and MS patients who did not have AQP-4-IgG. ConclusionsOur findings highlight that serological Interleukin-17B (IL-17B) may be key biomarker of NMOSD and MS. While epidermal growth factor (EGF) may be correlated with the breakdown of the blood-brain barrier in NMOSD patients, granulocyte chemotactic protein-2 (GCP-2) and monocyte differentiation antigen CD14 (CD14) may play different roles in the pathogenesis of AQP-4-IgG seronegative and seropositive NMOSD and MS. Novel biomarkers identified in our study could potentially be used in the diagnosis and treatment of NMOSD.Trial registrationPublic title: Multi-Center Clinical Study of GFAP AstrocytopathyRegistration number: ChiCTR2000041291Date of registration: 2020-12-23 (Retrospective registration)URL of trail registry record: http://www.chictr.org.cn/showproj.aspx?proj=65306

Immunocytochemical identification of the human α2-macrogiobulin receptor in monocytes and fibroblasts: Monoclonal antibodies define the receptor as a monocyte differentiation antigen

1990 ◽  
Vol 190 (2) ◽  
pp. 195-203 ◽  
Author(s):  
Søren Kragh Moestrup ◽  
Keld Kaltoft ◽  
Claus Munck Petersen ◽  
Søren Pedersen ◽  
Jørgen Gliemann ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Differentiation Antigen ◽  
Monocyte Differentiation
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