Regulated splicing of the Drosophila P transposable element third intron in vitro: somatic repression

Science ◽  
1990 ◽  
Vol 248 (4960) ◽  
pp. 1200-1208 ◽  
Author(s):  
C. Siebel ◽  
D. Rio
Keyword(s):  
Transposable Element ◽  
P Transposable Element

A genetic approach to the dissection of P transposable element functions in Drosophila

1984 ◽  
Vol 307 (1132) ◽  
pp. 231-238
Keyword(s):  
Transposable Element ◽  
Selectable Marker ◽  
Marker Gene ◽  
P Element ◽  
Genetic Approach ◽  
Selectable Marker Gene ◽  
P Elements ◽  
P Transposable Element

We have made a P element derivative that carries the selectable marker gene rosy, but which acts like a non-defective, intact P element. When introduced into an M strain fly, this element continues to transpose autonomously within the genome, and elicits certain other characteristics of hybrid dysgenesis. By mutagenizing the P element in vitro , and assaying its activity in vivo , both singly and in combination with other P elements, we have tentatively identified the regions of the element encoding the transposase function. In addition, we have examined the pattern of poly(A)+ transcripts encoded by P elements of natural P strains, and by our P element marked with rosy in transformed flies, and find two species that appear to be derived from non-defective elements, one of which may be the message for transposase.

scholarly journals Homology between P-transposable element of Drosophila melanogaster and bacterial transposase gene of Tn3.

1985 ◽  
Vol 60 (5) ◽  
pp. 499-503 ◽  
Author(s):  
Yoko SATTA ◽  
Takashi GOJOBORI ◽  
Takeo MARUYAMA ◽  
Kaoru SAIGO ◽  
Sadao I. CHIGUSA
Keyword(s):  
Drosophila Melanogaster ◽  
Transposable Element ◽  
Transposase Gene ◽  
P Transposable Element

scholarly journals Tn3 resolvase-like sequence in P transposable element of Drosophila melanogaster.

1985 ◽  
Vol 60 (3) ◽  
pp. 261-266 ◽  
Author(s):  
Yoko SATTA ◽  
Takashi GOJOBORI ◽  
Takeo MARUYAMA ◽  
Sadao I. CHIGUSA
Keyword(s):  
Drosophila Melanogaster ◽  
Transposable Element ◽  
P Transposable Element

scholarly journals Partial revertants of the transposable element-associated suppressible allele white-apricot in Drosophila melanogaster: structures and responsiveness to genetic modifiers.

Genetics ◽  
1988 ◽  
Vol 118 (2) ◽  
pp. 221-234
Author(s):  
S M Mount ◽  
M M Green ◽  
G M Rubin
Keyword(s):  
Transposable Element ◽  
P Element ◽  
Genetic Modifiers ◽  
Eye Color ◽  
Wild Type Phenotype ◽  
I Factor ◽  
White Locus ◽  
Modes Of Interaction ◽  
Copia Element

Abstract The eye color phenotype of white-apricot (wa), a mutant allele of the white locus caused by the insertion of the transposable element copia into a small intron, is suppressed by the extragenic suppressor suppressor-of-white-apricot (su(wa] and enhanced by the extragenic enhancers suppressor-of-forked su(f] and Enhancer-of-white-apricot (E(wa]. Derivatives of wa have been analyzed molecularly and genetically in order to correlate the structure of these derivatives with their response to modifiers. Derivatives in which the copia element is replaced precisely by a solo long terminal repeat (sLTR) were generated in vitro and returned to the germline by P-element mediated transformation; flies carrying this allele within a P transposon show a nearly wild-type phenotype and no response to either su(f) or su(wa). In addition, eleven partial phenotypic revertants of wa were analyzed. Of these, one appears to be a duplication of a large region which includes wa, three are new alleles of su(wa), two are sLTR derivatives whose properties confirm results obtained using transformation, and five are secondary insertions into the copia element within wa. One of these, waR84h, differs from wa by the insertion of the most 3' 83 nucleotides of the I factor. The five insertion derivatives show a variety of phenotypes and modes of interaction with su[f) and su(wa). The eye pigmentation of waR84h is affected by su(f) and E(wa), but not su(wa). These results demonstrate that copia (as opposed to the interruption of white sequences) is essential for the wa phenotype and its response to genetic modifiers, and that there are multiple mechanisms for the alteration of the wa phenotype by modifiers.

scholarly journals Models of repression of transposition in P-M hybrid dysgenesis by P cytotype and by zygotically encoded repressor proteins.

Genetics ◽  
1991 ◽  
Vol 128 (2) ◽  
pp. 471-486 ◽  
Author(s):  
J F Brookfield
Keyword(s):  
Computer Simulation ◽  
Transposable Elements ◽  
Transposable Element ◽  
Evolutionary Dynamics ◽  
Hybrid Dysgenesis ◽  
P Elements ◽  
Infinite Population ◽  
Repressor Proteins ◽  
P Transposable Element ◽  
Harmful Effects

Abstract By analytical theory and computer simulation the expected evolutionary dynamics of P transposable element spread in an infinite population are investigated. The analysis is based on the assumption that, unlike transposable elements which move via RNA intermediates, the harmful effects of P elements arise primarily in the act of transposition, and that this causes their evolutionary dynamics to be unusual. It is suggested that a situation of transposition-selection balance will be superceded by the buildup of a cytoplasmically inherited repression or by the elimination of active transposase-encoding elements from the chromosomes, a process which may be accompanied by the evolution of elements which encode proteins which repress transposition.

scholarly journals Somatic reversion of P transposable element insertion mutations in the singed locus of Drosophila melanogaster requiring specific P insertions and a trans- acting factor

1989 ◽  
Vol 54 (2) ◽  
pp. 101-112 ◽  
Author(s):  
John F. Y. Brookfield ◽  
Alan P. Lewis
Keyword(s):  
Drosophila Melanogaster ◽  
Transposable Element ◽  
Maternal Effect ◽  
P Element ◽  
Chromosome 2 ◽  
Restriction Maps ◽  
Element Insertion ◽  
Somatic Reversion ◽  
P Transposable Element ◽  
Insertion Mutations

SummaryDestabilization in somatic cells of P-element insertions in the X-linked singed gene of Drosophila melanogaster has been studied. We have shown that some but not all unstable P-element insertions in singed can form mosaics. The cause of this variation is not clear from studies of the restriction maps of the mutations tested. The transposable element movements occur early in development and require, in addition to an appropriate P-element insertion in singed, a trans-acting maternal effect component. Movements appear to occur preferentially in attached-X stocks. However, the maternal effect component maps to the central region of chromosome 2.

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