GPI-anchored cell-surface molecules complexed to protein tyrosine kinases

Science ◽  
1991 ◽  
Vol 254 (5034) ◽  
pp. 1016-1019 ◽  
Author(s):  
I Stefanova ◽  
V Horejsi ◽  
I. Ansotegui ◽  
W Knapp ◽  
H Stockinger
Keyword(s):  
Cell Surface ◽  
Tyrosine Kinases ◽  
Protein Tyrosine Kinases ◽  
Cell Surface Molecules ◽  
Protein Tyrosine ◽  
Surface Molecules

scholarly journals FAK and PYK2 are specifically associated with the activated phagocytic receptor complexes from human U937 cells.

2021 ◽  
Author(s):  
Marwan G. AbidAlthagafi
Keyword(s):  
Cell Surface ◽  
Tyrosine Kinases ◽  
Laser Scanning ◽  
Surface Proteins ◽  
Protein Tyrosine Kinases ◽  
Macrophage Cell ◽  
Protein Tyrosine ◽  
Surface Receptors ◽  
Receptor Complexes

The innate immune system is the first shield against foreign attack inside the human body, and it is usually carried out with phagocytosis. An essential macrophage cell surface protein is the Fc receptor which contributes to the engulfment of unknown antigens. One of the important members of Fc receptors is the gamma receptor that binds to the immunoglobulin G (IgG) ligand. Another key receptor in this study is the CD36 receptor, which plays a crucial role in the progression of atherosclerosis, the hardening of arteries, with its ligand oxidized low-density lipoprotein (OxLDL). In this report, protein tyrosine kinase enzymes have been detected in the involvement of receptor complexes with human U937 macrophages, specifically PTK2 and PTK2b genes. Protein tyrosine kinases were known to promote cell migration as a main player in intracellular signal transduction cascades in relation to extracellular stimuli. Cell surface proteins are essential for the immunization of various diseases; yet, the molecular machinery of surface receptors remains unclear. This research primarily examined the dynamic nature of protein tyrosine kinases in an ongoing investigation of macrophage cell surface receptors, particularly the role of Fc γ and CD36 receptors with their ligands IgG and oxLDL coated beads in phagocytosis. Our report demonstrates a novel role of PTK2 and PTK2b functions in relation to U937 CD36-mediated phagocytosis. The Phagocytic efficiency of U937 macrophages was analyzed using laser scanning confocal microscope after silencing the cells with siRNA followed by quantitative counting of phagocytosis. The PF drug FAK inhibitor was also introduced to compare the phagocytic efficiency of siRNA cells.

Association of Src-like protein tyrosine kinases with the CD2 cell surface molecule in rat T lymphocytes and natural killer cells

1992 ◽  
Vol 12 (12) ◽  
pp. 5548-5554
Author(s):  
G M Bell ◽  
J B Bolen ◽  
J B Imboden
Keyword(s):  
Natural Killer Cells ◽  
T Lymphocytes ◽  
Cell Surface ◽  
Natural Killer ◽  
Tyrosine Kinases ◽  
Protein Tyrosine Kinases ◽  
Surface Molecule ◽  
Cell Surface Molecule ◽  
Killer Cells ◽  
Protein Tyrosine

The cell surface molecule CD2 has a signaling role in the activation of T lymphocytes and natural killer cells. Because perturbation of CD2 leads to the appearance of tyrosine-phosphorylated proteins, we investigated the possibility that CD2 associates with cytoplasmic protein tyrosine kinases. As determined by in vitro kinase assays and phosphoamino acid analysis, protein tyrosine kinase activity coprecipitated with CD2 from rat T lymphocytes, T lymphoblasts, thymocytes, interleukin-2-activated natural killer cells, and RNK-16 cells (a rat natural killer cell line). In each case, both p56lck and p59fyn were identified in the CD2 immunoprecipitate. In the thymus, the association between CD2 and these kinases occurred predominately in a small subset of thymocytes that had the cell surface phenotype of mature T cells, indicating that the association is a regulated event and occurs late in T-cell ontogeny. The finding that CD2 is associated with p56lck and p59fyn in detergent lysates suggests that interactions with these Src-like protein kinases play a critical role in CD2-mediated signal transduction.

scholarly journals FAK and PYK2 are specifically associated with the activated phagocytic receptor complexes from human U937 cells.

2021 ◽  
Author(s):  
Marwan G. AbidAlthagafi
Keyword(s):  
Cell Surface ◽  
Tyrosine Kinases ◽  
Laser Scanning ◽  
Surface Proteins ◽  
Protein Tyrosine Kinases ◽  
Macrophage Cell ◽  
Protein Tyrosine ◽  
Surface Receptors ◽  
Receptor Complexes

The innate immune system is the first shield against foreign attack inside the human body, and it is usually carried out with phagocytosis. An essential macrophage cell surface protein is the Fc receptor which contributes to the engulfment of unknown antigens. One of the important members of Fc receptors is the gamma receptor that binds to the immunoglobulin G (IgG) ligand. Another key receptor in this study is the CD36 receptor, which plays a crucial role in the progression of atherosclerosis, the hardening of arteries, with its ligand oxidized low-density lipoprotein (OxLDL). In this report, protein tyrosine kinase enzymes have been detected in the involvement of receptor complexes with human U937 macrophages, specifically PTK2 and PTK2b genes. Protein tyrosine kinases were known to promote cell migration as a main player in intracellular signal transduction cascades in relation to extracellular stimuli. Cell surface proteins are essential for the immunization of various diseases; yet, the molecular machinery of surface receptors remains unclear. This research primarily examined the dynamic nature of protein tyrosine kinases in an ongoing investigation of macrophage cell surface receptors, particularly the role of Fc γ and CD36 receptors with their ligands IgG and oxLDL coated beads in phagocytosis. Our report demonstrates a novel role of PTK2 and PTK2b functions in relation to U937 CD36-mediated phagocytosis. The Phagocytic efficiency of U937 macrophages was analyzed using laser scanning confocal microscope after silencing the cells with siRNA followed by quantitative counting of phagocytosis. The PF drug FAK inhibitor was also introduced to compare the phagocytic efficiency of siRNA cells.

scholarly journals Association of Src-like protein tyrosine kinases with the CD2 cell surface molecule in rat T lymphocytes and natural killer cells.

1992 ◽  
Vol 12 (12) ◽  
pp. 5548-5554 ◽  
Author(s):  
G M Bell ◽  
J B Bolen ◽  
J B Imboden
Keyword(s):  
Natural Killer Cells ◽  
T Lymphocytes ◽  
Cell Surface ◽  
Natural Killer ◽  
Tyrosine Kinases ◽  
Protein Tyrosine Kinases ◽  
Surface Molecule ◽  
Cell Surface Molecule ◽  
Killer Cells ◽  
Protein Tyrosine

The cell surface molecule CD2 has a signaling role in the activation of T lymphocytes and natural killer cells. Because perturbation of CD2 leads to the appearance of tyrosine-phosphorylated proteins, we investigated the possibility that CD2 associates with cytoplasmic protein tyrosine kinases. As determined by in vitro kinase assays and phosphoamino acid analysis, protein tyrosine kinase activity coprecipitated with CD2 from rat T lymphocytes, T lymphoblasts, thymocytes, interleukin-2-activated natural killer cells, and RNK-16 cells (a rat natural killer cell line). In each case, both p56lck and p59fyn were identified in the CD2 immunoprecipitate. In the thymus, the association between CD2 and these kinases occurred predominately in a small subset of thymocytes that had the cell surface phenotype of mature T cells, indicating that the association is a regulated event and occurs late in T-cell ontogeny. The finding that CD2 is associated with p56lck and p59fyn in detergent lysates suggests that interactions with these Src-like protein kinases play a critical role in CD2-mediated signal transduction.

Naturally Occurring Homoisoflavonoids as Potent Protein Tyrosine Kinases Inhibitors

Planta Medica ◽  
2008 ◽  
Vol 74 (03) ◽  
Author(s):  
Y Ye ◽  
LG Lin ◽  
H Xie ◽  
HL Li ◽  
HL Jiang ◽  
...  
Keyword(s):  
Tyrosine Kinases ◽  
Protein Tyrosine Kinases ◽  
Protein Tyrosine ◽  
Naturally Occurring

Differential Effects of Somatostatin and Its Analog on Protein Tyrosine Kinases Activity in the Rat Pituitary and the Murine Colonic Tumors

1998 ◽  
Vol 246 (2) ◽  
pp. 375-377 ◽  
Author(s):  
Marek Pawlikowski ◽  
Lilla Lachowicz ◽  
Jolanta Kunert-Radek ◽  
Katarzyna Winczyk ◽  
Grażyna Janiszewska ◽  
...  
Keyword(s):  
Tyrosine Kinases ◽  
Protein Tyrosine Kinases ◽  
Differential Effects ◽  
Protein Tyrosine ◽  
Rat Pituitary

scholarly journals Cell surface molecules that bind fibronectin's matrix assembly domain

1991 ◽  
Vol 266 (15) ◽  
pp. 9697-9702 ◽  
Author(s):  
A.H. Limper ◽  
B.J. Quade ◽  
R.M. LaChance ◽  
T.M. Birkenmeier ◽  
T.S. Rangwala ◽  
...  
Keyword(s):  
Cell Surface ◽  
Cell Surface Molecules ◽  
Matrix Assembly ◽  
Surface Molecules
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