Immune Response in vitro: Independence of "Activated" Lymphoid Cells

Science ◽  
1969 ◽  
Vol 166 (3908) ◽  
pp. 1002-1004 ◽  
Author(s):  
C. W. Pierce ◽  
B. Benacerraf
Keyword(s):  
Immune Response ◽  
Lymphoid Cells ◽  
Immune Response In Vitro

scholarly journals CELL-MEDIATED IMMUNE RESPONSE IN VITRO

1972 ◽  
Vol 136 (2) ◽  
pp. 331-343 ◽  
Author(s):  
Hermann Wagner ◽  
Marc Feldmann ◽  
W. Boyle ◽  
J. W. Schrader
Keyword(s):  
Immune Response ◽  
Peritoneal Macrophages ◽  
Lymphoid Cells ◽  
Cytotoxic Lymphocytes ◽  
Separation Procedure ◽  
Macrophage Depletion ◽  
Cell Mediated Immune Response ◽  
Immune Response In Vitro ◽  
Allograft Response

All efficient cell separation procedure and specific anti-macrophage serum were used to investigate the requirement of macrophages in the in vitro allograft response of mouse lymphoid cells. The efficiency of the macrophage-depletion procedure used and the undiminished capacity of the purified lymphocytes to respond were verified by also testing the antibody responses to sheep red cells (SRC) and dinitrophenylated polymeric flagellin (DNP POL) as well as the proliferative response to allogeneic cells. It was found that the generation of cytotoxic lymphocytes were diminished after macrophage depletion by surface adherence. The combination of anti-macrophage serum and column purification resulted in the total abolition of cytotoxic activity. The cell-mediated immune response was restored completely by addition of peritoneal macrophages, with as few as 1 macrophage to 600 lymphocytes permitting a significant restoration. Macrophages were not involved in the cytotoxic effector phase, but were essential in immune induction. A subcellular H-2 alloantigen preparation was only immunogenic in the presence of macrophages, indicating that a mere reduction in the size of the antigen from cell-bound alloantigens to membrane fragments was not the sole function of macrophages. The results suggest that macrophages collaborate with T cells in the initiation of an allograft response in vitro.

scholarly journals STAT3 Knockdown in B16 Melanoma by siRNA Lipopolyplexes Induces Bystander Immune Response In Vitro and In Vivo

2011 ◽  
Vol 4 (3) ◽  
pp. 178-188 ◽  
Author(s):  
Aws Alshamsan ◽  
Samar Hamdy ◽  
Azita Haddadi ◽  
John Samuel ◽  
Ayman O.S. El-Kadi ◽  
...  
Keyword(s):  
Immune Response ◽  
B16 Melanoma ◽  
Immune Response In Vitro

W1612 Apical Exposure of Intestinal Epithelial Cells to Lactobacilli But Not Bifidobacteria Enhances the Effector Immune Response In Vitro

2009 ◽  
Vol 136 (5) ◽  
pp. A-702
Author(s):  
Els van Hoffen ◽  
Nicoline M. Korthagen ◽  
Sander de Kivit ◽  
Bastiaan Schouten ◽  
Bart Bardoel ◽  
...  
Keyword(s):  
Immune Response ◽  
Epithelial Cells ◽  
Intestinal Epithelial Cells ◽  
Intestinal Epithelial ◽  
Effector Immune Response ◽  
Immune Response In Vitro

scholarly journals Polystyrene nanoplastics accumulate in ZFL cell lysosomes and in zebrafish larvae after acute exposure, inducing a synergistic immune response in vitro without affecting larval survival in vivo

2020 ◽  
Vol 7 (8) ◽  
pp. 2410-2422
Author(s):  
Irene Brandts ◽  
Marlid Garcia-Ordoñez ◽  
Lluis Tort ◽  
Mariana Teles ◽  
Nerea Roher
Keyword(s):  
Immune Response ◽  
Larval Survival ◽  
Liver Cells ◽  
Acute Exposure ◽  
Zebrafish Larvae ◽  
Lethal Infection ◽  
Zebrafish Liver ◽  
Immune Response In Vitro

Polystyrene nanoplastics are internalized in zebrafish liver cells, accumulating in lysosomes, and in zebrafish larvae but do not affect the larval suvival to a lethal infection.

scholarly journals Can levamisole upregulate the equine cell‐mediated macrophage (M1) dendritic cell (DC1) T‐helper 1 (CD4 Th1) T‐cytotoxic (CD8) immune response in vitro?

2019 ◽  
Vol 33 (2) ◽  
pp. 889-896 ◽  
Author(s):  
Sharon Witonsky ◽  
Virginia Buechner‐Maxwell ◽  
Amy Santonastasto ◽  
Robert Pleasant ◽  
Stephen Werre ◽  
...  
Keyword(s):  
Immune Response ◽  
Dendritic Cell ◽  
T Helper ◽  
T Helper 1 ◽  
Immune Response In Vitro

Abstract C056: MIV-818 stimulates an antitumor immune response in vitro and enhances the effects of pembrolizumab

2019 ◽  
Author(s):  
Fredrik G Oberg ◽  
Karin Tunblad ◽  
Ana Slipicevic ◽  
Richard Bethell ◽  
Mark R Albertella
Keyword(s):  
Immune Response ◽  
Antitumor Immune Response ◽  
Immune Response In Vitro
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