Bacteriophage lambda PaPa: not the mother of all lambda phages

Science ◽  
1992 ◽  
Vol 258 (5085) ◽  
pp. 1145-1148 ◽  
Author(s):  
R. Hendrix ◽  
R. Duda
Keyword(s):  
Bacteriophage Lambda ◽  
Lambda Phages

scholarly journals The effects of central asymmetry on the propagation of palindromic DNA in bacteriophage lambda are consistent with cruciform extrusion in vivo.

Genetics ◽  
1993 ◽  
Vol 133 (2) ◽  
pp. 143-148 ◽  
Author(s):  
A F Chalker ◽  
E A Okely ◽  
A Davison ◽  
D R Leach
Keyword(s):  
Dna Sequences ◽  
Bacteriophage Lambda ◽  
Base Pairs ◽  
Lambda Phages

Abstract The propagation of lambda phages carrying long perfect palindromes has been compared with that of phages carrying imperfect palindromes with small regions of central asymmetry. The perfect palindromes confer a more deleterious phenotype than those with central asymmetry and the severity of the phenotype declines with the length of asymmetry in the range from O to 27 base pairs. These results argue that a center-dependent reaction is involved in the phenotypic effects of palindromic DNA sequences, consistent with the idea that cruciform extrusion occurs in vivo.

scholarly journals Bacteriophage lambda N gene leader RNA. RNA processing and translational initiation signals.

1987 ◽  
Vol 262 (36) ◽  
pp. 17651-17658
Author(s):  
D A Steege ◽  
K C Cone ◽  
C Queen ◽  
M Rosenberg
Keyword(s):  
Rna Processing ◽  
Bacteriophage Lambda ◽  
N Gene ◽  
Translational Initiation

scholarly journals Interference by PR-bound RNA polymerase with PRM function in vitro. Modulation by the bacteriophage lambda cI protein.

1993 ◽  
Vol 268 (12) ◽  
pp. 8943-8948
Author(s):  
P.A. Hershberger ◽  
B.C. Mita ◽  
A. Tripatara ◽  
P.L. deHaseth
Keyword(s):  
Rna Polymerase ◽  
Bacteriophage Lambda

scholarly journals THE INTERACTION OF cos WITH CHI IS SEPARABLE FROM DNA PACKAGING IN recA-recBC-MEDIATED RECOMBINATION OF BACTERIOPHAGE LAMBDA

Genetics ◽  
1983 ◽  
Vol 104 (4) ◽  
pp. 549-570
Author(s):  
Ichizo Kobayashi ◽  
Mary M Stahl ◽  
David Leach ◽  
Franklin W Stahl
Keyword(s):  
Escherichia Coli ◽  
Bacteriophage Lambda ◽  
Dna Packaging ◽  
Bacteriophage Λ ◽  
Entry Site ◽  
Two Component ◽  
General Recombination

ABSTRACT Chi (5′-GCTGGTGG) is a recombinator in RecA- RecBC-mediated recombination in Escherichia coli. In bacteriophage λ vegetative recombination, Chi is fully active only when it is correctly oriented with respect to cos, the site that defines the ends of the packaged chromosome. Here we demonstrate that packaging from cos is not necessary for this cos-Chi interaction. Our evidence suggests that correctly oriented cos is an activator of Chi. cos, as an activator, is (1) dominant over cos  -, (2) active opposite an extensive heterology, (3) able to interact with Chi only when on the same (cis) chromosome, and (4) able to interact with Chi at distances as far as ≥ 20 kb. Thus, cos and Chi form a two-component recombinator system for general recombination. cos may serve as an asymmetric entry site for a recombination enzyme that recognizes Chi in an asymmetric way.

The N protein of bacteriophage lambda, defined by its DNA sequence, is highly basic

Gene ◽  
1979 ◽  
Vol 8 (1) ◽  
pp. 107-119 ◽  
Author(s):  
N.C. Franklin ◽  
G.N. Bennett
Keyword(s):  
Dna Sequence ◽  
Bacteriophage Lambda ◽  
N Protein

scholarly journals Phage genetic sites involved in lambda growth inhibition by the Escherichia coli rap mutant.

Genetics ◽  
1989 ◽  
Vol 121 (3) ◽  
pp. 401-409
Author(s):  
P Guzmán ◽  
G Guarneros
Keyword(s):  
Escherichia Coli ◽  
Growth Inhibition ◽  
Phage Genome ◽  
Bacteriophage Lambda ◽  
Phage Lambda ◽  
Wild Type ◽  
Attp Site

Abstract The rap mutation of Escherichia coli prevents the growth of bacteriophage lambda. We have isolated phage mutants that compensate for the host deficiency. The mutations, named bar, were genetically located to three different loci of the lambda genome: barI in the attP site, barII in the cIII ea10 region, and barIII within or very near the imm434 region. The level of lambda leftward transcription correlates with rap exclusion. Phage lambda mutants partially defective in the pL promoter or in pL-transcript antitermination showed a Bar- phenotype. Conversely, mutants constitutive for transcription from the pI or pL promoters were excluded more stringently by rap bacteria. We conclude that rap exclusion depends on the magnitude of transcription through the wild type bar loci in the phage genome.

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