Impeding Xist Expression from the Active X Chromosome Improves Mouse Somatic Cell Nuclear Transfer

Science ◽  
2010 ◽  
Vol 330 (6003) ◽  
pp. 496-499 ◽  
Author(s):  
K. Inoue ◽  
T. Kohda ◽  
M. Sugimoto ◽  
T. Sado ◽  
N. Ogonuki ◽  
...  
Keyword(s):  
Somatic Cell ◽  
X Chromosome ◽  
Somatic Cell Nuclear Transfer ◽  
Nuclear Transfer ◽  
Xist Expression ◽  
Mouse Somatic Cell

Methyl-CpG–Binding Protein 2 Improves the Development of Mouse Somatic Cell Nuclear Transfer Embryos

2016 ◽  
Vol 18 (2) ◽  
pp. 78-86 ◽  
Author(s):  
Zhen-Dong Wang ◽  
Lian Duan ◽  
Zi-Hui Zhang ◽  
Si-Hang Song ◽  
Guang-Yu Bai ◽  
...  
Keyword(s):  
Somatic Cell ◽  
Somatic Cell Nuclear Transfer ◽  
Nuclear Transfer ◽  
Binding Protein ◽  
Mouse Somatic Cell

Highly methylated Xist in SCNT embryos was retained in deceased cloned female goats

2019 ◽  
Vol 31 (5) ◽  
pp. 855 ◽  
Author(s):  
Mingtian Deng ◽  
Zifei Liu ◽  
Caifang Ren ◽  
Shiyu An ◽  
Yongjie Wan ◽  
...  
Keyword(s):  
Somatic Cell ◽  
Brain Tissue ◽  
Embryo Development ◽  
Intracytoplasmic Sperm Injection ◽  
Somatic Cell Nuclear Transfer ◽  
Nuclear Transfer ◽  
Methylation Status ◽  
Differentially Methylated Region ◽  
Xist Expression ◽  
Specific Transcript

X (inactive)-specific transcript (Xist) is crucial in murine cloned embryo development, but its role in cloned goats remains unknown. Therefore, in this study we examined the expression and methylation status of Xist in somatic cell nuclear transfer (SCNT) embryos, as well as in ear, lung, and brain tissue of deceased cloned goats. First, the Xist sequence was amplified and a differentially methylated region was identified in oocytes and spermatozoa. Xist methylation levels were greater in SCNT- than intracytoplasmic sperm injection-generated female 8-cell embryos. In addition, compared with naturally bred controls, Xist methylation levels were significantly increased in the ear, lung, and brain tissue of 3-day-old female deceased cloned goats, but were unchanged in the ear tissue of female live cloned goats and in the lung and brain of male deceased cloned goats. Xist expression was significantly increased in the ear tissue of female live cloned goats, but decreased in the lung and brain of female deceased cloned goats. In conclusion, hypermethylation of Xist may have resulted from incomplete reprogramming and may be retained in 3-day-old female deceased cloned goats, subsequently leading to dysregulation of Xist.

Effects of Embryo Aggregation and PXD101 on the In Vitro Development of Mouse Somatic Cell Nuclear Transfer Embryos

2017 ◽  
Vol 19 (6) ◽  
pp. 337-343 ◽  
Author(s):  
Xiaoyan Qiu ◽  
Nan Li ◽  
Xiong Xiao ◽  
Liang Zhang ◽  
Haihong You ◽  
...  
Keyword(s):  
Somatic Cell ◽  
Somatic Cell Nuclear Transfer ◽  
Nuclear Transfer ◽  
In Vitro Development ◽  
Vitro Development ◽  
Mouse Somatic Cell

Effect of melatonin treatment on developmental potential of somatic cell nuclear-transferred mouse oocytes in vitro

Zygote ◽  
2013 ◽  
Vol 22 (2) ◽  
pp. 213-217 ◽  
Author(s):  
Mohammad Salehi ◽  
Yoko Kato ◽  
Yukio Tsunoda
Keyword(s):  
Somatic Cell ◽  
Somatic Cell Nuclear Transfer ◽  
Nuclear Transfer ◽  
Culture Medium ◽  
Blastocyst Stage ◽  
Control Group ◽  
Developmental Potential ◽  
Significant Difference ◽  
Mouse Somatic Cell

SummaryThe beneficial effect of supplementing culture medium with melatonin has been reported during in vitro embryo development of species such as mouse, bovine and porcine. However, the effect of melatonin on mouse somatic cell nuclear transfer remains unknown. In this study, we assessed the effects of various concentrations of melatonin (10−6 to 10−12 M) on the in vitro development of mouse somatic cell nuclear transfer embryos for 96 h. Embryos cultured without melatonin were used as control. There was no significant difference in cleavage rates between the groups supplemented with melatonin, dimethyl sulphoxide (DMSO) and the control. The rate of development to blastocyst stage was significantly higher in the group supplemented with 10−12 M melatonin compared with the control group (P < 0.05). Thus, our data demonstrated that adding melatonin to pre-implantation mouse nuclear-transferred embryos can accelerate blastocyst formation.

scholarly journals XIST Derepression in Active X Chromosome Hinders Pig Somatic Cell Nuclear Transfer

2018 ◽  
Vol 10 (2) ◽  
pp. 494-508 ◽  
Author(s):  
Degong Ruan ◽  
Jiangyun Peng ◽  
Xiaoshan Wang ◽  
Zhen Ouyang ◽  
Qingjian Zou ◽  
...  
Keyword(s):  
Somatic Cell ◽  
X Chromosome ◽  
Somatic Cell Nuclear Transfer ◽  
Nuclear Transfer
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