scholarly journals Substrate Elasticity Regulates Skeletal Muscle Stem Cell Self-Renewal in Culture

Science ◽  
2010 ◽  
Vol 329 (5995) ◽  
pp. 1078-1081 ◽  
Author(s):  
P. M. Gilbert ◽  
K. L. Havenstrite ◽  
K. E. G. Magnusson ◽  
A. Sacco ◽  
N. A. Leonardi ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Stem Cell ◽  
Self Renewal ◽  
Muscle Stem Cell ◽  
Substrate Elasticity

scholarly journals Skeletal muscle stem cell self-renewal and differentiation kinetics revealed by EdU lineage tracing during regeneration

2019 ◽  
Author(s):  
Bradley Pawlikowski ◽  
Nicole Dalla Betta ◽  
Tiffany Antwine ◽  
Bradley B. Olwin
Keyword(s):  
Skeletal Muscle ◽  
Stem Cells ◽  
Stem Cell ◽  
Young Adult ◽  
Muscle Injury ◽  
Lineage Tracing ◽  
Stem Cell Population ◽  
Post Injury ◽  
Self Renewal ◽  
Muscle Stem Cell

SummarySkeletal muscle maintenance and repair is dependent on the resident adult muscle stem cell (MuSC). During injury, and in diseased muscle, stem cells are engaged to replace or repair damaged muscle, which requires the stem cells to exit quiescence and expand, followed by differentiation to regenerate myofibers and self-renewal to replenish the stem cell population. Following an injury, little is known regarding the timing of MuSC (skeletal muscle stem cell) self-renewal, myoblast expansion or myoblast differentiation. To determine the timing and kinetics of these cell fate decisions, we employed DNA-based lineage tracing to label newly replicated cells and followed cell fates during skeletal muscle regeneration. MuSCs activate and expand as myoblasts rapidly following injury, where the majority differentiate into myonuclei, establishing the centrally located myonuclear pool. Re-establishing the majority MuSC pool by self-renewal occurs after 5 days post-muscle injury, accompanied by low levels of myonuclear accretion that generate only peripheral myonuclei. In aged mice, possessing ∼1/2 the number of MuSCs present in young adult mice, the timing of post injury MuSC self-renewal is delayed, and although MuSCs expansion as myoblasts in aged muscle is impaired, the number of MuSC unexpectedly recovers to young adult levels during regeneration. Following an induced muscle injury, we found that myonuclei are generated within the first four days post injury derived from myoblasts expanding from activated MuSCs. Only later during regeneration, from 5 d to 14 d post injury, is the MuSC pool replenished by self-renewal, accompanied by generation of peripheral myonuclei.

The Regenerating Skeletal Muscle Niche Guides Muscle Stem Cell Self-Renewal

2021 ◽  
Author(s):  
Alicia Ann Cutler ◽  
Bradley Pawlikowski ◽  
Joshua R. Wheeler ◽  
Nicole Carol Dalla Betta ◽  
Tiffany Antwine ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Stem Cell ◽  
Self Renewal ◽  
Muscle Stem Cell

scholarly journals Basal lamina remodeling at the skeletal muscle stem cell niche mediates stem cell self-renewal

2018 ◽  
Vol 9 (1) ◽  
Author(s):  
Shantisree Sandeepani Rayagiri ◽  
Daniele Ranaldi ◽  
Alexander Raven ◽  
Nur Izzah Farhana Mohamad Azhar ◽  
Olivier Lefebvre ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Stem Cell ◽  
Basal Lamina ◽  
Stem Cell Niche ◽  
Self Renewal ◽  
Muscle Stem Cell ◽  
Cell Niche

The Regenerating Skeletal Muscle Niche Guides Muscle Stem Cell Self-Renewal

2021 ◽  
Author(s):  
Alicia Ann Cutler ◽  
Bradley Pawlikowski ◽  
Joshua R. Wheeler ◽  
Nicole Carol Dalla Betta ◽  
Tiffany Antwine ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Stem Cell ◽  
Self Renewal ◽  
Muscle Stem Cell

Stem Cell Function, Self-Renewal, Heterogeneity, and Regenerative Potential in Skeletal Muscle Stem Cells

2012 ◽  
Vol 2 (1) ◽  
pp. 11-21
Author(s):  
Silvia Cristini ◽  
Giulio Alessandri ◽  
Francesco Acerbi ◽  
Daniela Tavian ◽  
Eugenio A. Parati ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Stem Cells ◽  
Stem Cell ◽  
Cell Function ◽  
Self Renewal ◽  
Muscle Stem Cells ◽  
Skeletal Muscle Stem Cells
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