scholarly journals Motile Cilia of Human Airway Epithelia Are Chemosensory

Science ◽  
2009 ◽  
Vol 325 (5944) ◽  
pp. 1131-1134 ◽  
Author(s):  
A. S. Shah ◽  
Y. Ben-Shahar ◽  
T. O. Moninger ◽  
J. N. Kline ◽  
M. J. Welsh
Keyword(s):  
Airway Epithelia ◽  
Human Airway ◽  
Motile Cilia ◽  
Human Airway Epithelia

scholarly journals Characterization and Treatment of SARS-CoV-2 in Nasal and Bronchial Human Airway Epithelia

2020 ◽  
Vol 1 (4) ◽  
pp. 100059 ◽  
Author(s):  
Andrés Pizzorno ◽  
Blandine Padey ◽  
Thomas Julien ◽  
Sophie Trouillet-Assant ◽  
Aurélien Traversier ◽  
...  
Keyword(s):  
Airway Epithelia ◽  
Human Airway ◽  
Human Airway Epithelia

scholarly journals Chronic β2AR stimulation limits CFTR activation in human airway epithelia

JCI Insight ◽  
2018 ◽  
Vol 3 (4) ◽  
Author(s):  
John J. Brewington ◽  
Jessica Backstrom ◽  
Amanda Feldman ◽  
Elizabeth L. Kramer ◽  
Jessica D. Moncivaiz ◽  
...  
Keyword(s):  
Airway Epithelia ◽  
Human Airway ◽  
Human Airway Epithelia

scholarly journals Propagation of respiratory viruses in human airway epithelia reveals persistent virus-specific signatures

2018 ◽  
Vol 141 (6) ◽  
pp. 2074-2084 ◽  
Author(s):  
Manel Essaidi-Laziosi ◽  
Francisco Brito ◽  
Sacha Benaoudia ◽  
Léna Royston ◽  
Valeria Cagno ◽  
...  
Keyword(s):  
Respiratory Viruses ◽  
Airway Epithelia ◽  
Human Airway ◽  
Persistent Virus ◽  
Human Airway Epithelia

Hairpin transfer-independent Parvovirus DNA Replication Produces Infectious Virus

2021 ◽  
Author(s):  
Weiran Shen ◽  
Zekun Wang ◽  
Kang Ning ◽  
Fang Cheng ◽  
John F. Engelhardt ◽  
...  
Keyword(s):  
Dna Replication ◽  
Hek293 Cells ◽  
Progeny Virus ◽  
Human Bocavirus ◽  
Viral Dna ◽  
Airway Epithelia ◽  
Viral Dna Replication ◽  
Human Airway ◽  
The Right ◽  
Human Airway Epithelia

Parvoviruses package a linear single-stranded DNA genome with hairpin structures at both ends. It has been thought that terminal hairpin sequences are indispensable for viral DNA replication. Here, we provide evidence that the hairpin-deleted duplex genomes of human bocavirus 1 (HBoV1) replicate in human embryonic kidney (HEK) 293 cells. We propose an alternative model for HBoV1 DNA replication in which the leading strand can initiate strand-displacement without “hairpin-transfer.” The transfection of the HBoV1 duplex genomes that retain a minimal replication origin at the right-end ( OriR ), but with extensive deletions in the right-end hairpin (REH), generated viruses in HEK293 cells at a level 10-20 times lower than the wild-type (WT) duplex genome. Importantly, these viruses that have a genome with various deletions after the OriR , but not the one retaining only the OriR , replicated in polarized human airway epithelia. We discovered that the 18-nt sequence (nt 5,403-5,420) beyond the OriR was sufficient to confer virus replication in polarized human airway epithelia, although its progeny virus production was ∼5 times lower than that of the WT virus. Thus, our study demonstrates that hairpin transfer-independent productive parvovirus DNA replication can occur. Importance Hairpin transfer-independent parvovirus replication was modeled with human bocavirus 1 (HBoV1) duplex genomes whose 5’ hairpin structure was ablated by various deletions. In HEK293 cells, these duplex viral genomes with ablated 5’/hairpin sequence replicated efficiently and generated viruses that productively infected polarized human airway epithelium. Thus, for the first time, we reveal a previously unknown phenomenon that the productive parvovirus DNA replication does not depend on the hairpin sequence at REH to initiate “rolling hairpin” DNA replication. Notably, the intermediates of viral DNA replication, as revealed two-dimensional electrophoresis, from transfections of hairpin sequence-deleted duplex genome and full-length genome in HEK293 cells, as well as from virus infection of polarized human airway epithelia are similar. Thus, the establishment of the hairpin transfer-independent parvoviral DNA replication deepens our understanding in viral DNA replication and may have implications in development of parvovirus-based viral vectors with alternative properties.

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