Structural Basis of DNA Replication Origin Recognition by an ORC Protein

Science ◽  
2007 ◽  
Vol 317 (5842) ◽  
pp. 1213-1216 ◽  
Author(s):  
M. Gaudier ◽  
B. S. Schuwirth ◽  
S. L. Westcott ◽  
D. B. Wigley
Keyword(s):  
Dna Replication ◽  
Replication Origin ◽  
Structural Basis ◽  
Dna Replication Origin ◽  
Origin Recognition

scholarly journals Structural basis of DNA replication origin recognition by human Orc6 protein binding with DNA

2020 ◽  
Vol 48 (19) ◽  
pp. 11146-11161
Author(s):  
Naining Xu ◽  
Yingying You ◽  
Changdong Liu ◽  
Maxim Balasov ◽  
Lee Tung Lun ◽  
...  
Keyword(s):  
Dna Replication ◽  
Dna Binding ◽  
Solution Structure ◽  
Origin Recognition Complex ◽  
Structural Basis ◽  
Dna Replication Origin ◽  
Dna Alterations ◽  
Replication Initiator ◽  
Binding Subunit ◽  
Origin Recognition

Abstract The six-subunit origin recognition complex (ORC), a DNA replication initiator, defines the localization of the origins of replication in eukaryotes. The Orc6 subunit is the smallest and the least conserved among ORC subunits. It is required for DNA replication and essential for viability in all species. Orc6 in metazoans carries a structural homology with transcription factor TFIIB and can bind DNA on its own. Here, we report a solution structure of the full-length human Orc6 (HsOrc6) alone and in a complex with DNA. We further showed that human Orc6 is composed of three independent domains: N-terminal, middle and C-terminal (HsOrc6-N, HsOrc6-M and HsOrc6-C). We also identified a distinct DNA-binding domain of human Orc6, named as HsOrc6-DBD. The detailed analysis of the structure revealed novel amino acid clusters important for the interaction with DNA. Alterations of these amino acids abolish DNA-binding ability of Orc6 and result in reduced levels of DNA replication. We propose that Orc6 is a DNA-binding subunit of human/metazoan ORC and may play roles in targeting, positioning and assembling the functional ORC at the origins.

Structure of the origin recognition complex bound to DNA replication origin

Nature ◽  
2018 ◽  
Vol 559 (7713) ◽  
pp. 217-222 ◽  
Author(s):  
Ningning Li ◽  
Wai Hei Lam ◽  
Yuanliang Zhai ◽  
Jiaxuan Cheng ◽  
Erchao Cheng ◽  
...  
Keyword(s):  
Dna Replication ◽  
Replication Origin ◽  
Origin Recognition Complex ◽  
Dna Replication Origin ◽  
Origin Recognition

Identification and characterization of the DNA replication origin recognition complex gene family in the silkworm Bombyx mori

2011 ◽  
Vol 31 (5) ◽  
pp. 353-361 ◽  
Author(s):  
Hui-Peng Yang ◽  
Su-Juan Luo ◽  
Yi-Nü Li ◽  
Yao-Zhou Zhang ◽  
Zhi-Fang Zhang
Keyword(s):  
Dna Replication ◽  
Bombyx Mori ◽  
Replication Origin ◽  
Origin Recognition Complex ◽  
Bioinformatic Analysis ◽  
Replication Complex ◽  
Dna Replication Origin ◽  
Rapid Amplification ◽  
Replication Factors ◽  
Origin Recognition

The ORC (origin recognition complex) binds to the DNA replication origin and recruits other replication factors to form the pre-replication complex. The cDNA and genomic sequences of all six subunits of ORC in Bombyx mori (BmORC1–6) were determined by RACE (rapid amplification of cDNA ends) and bioinformatic analysis. The conserved domains were identified in BmOrc1p–6p and the C-terminal of BmOrc6p features a short sequence that may be specific for Lepidoptera. As in other organisms, each of the six BmORC subunits had evolved individually from ancestral genes in early eukaryotes. During embryo development, the six genes were co-regulated, but different ratios of the abundance of mRNAs were observed in 13 tissues of the fifth instar day-6 larvae. Infection by BmNPV (B. mori nucleopolyhedrovirus) initially decreased and then increased the abundance of BmORC. We suggest that some of the BmOrc proteins may have additional functions and that BmOrc proteins participate in the replication of BmNPV.

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